Q5M5K5 (KHSE_STRT2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homoserine kinase
Short name=HK
Short name=HSK
EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	stu0470

Organism

Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311) [Complete proteome] [HAMAP]

Taxonomic identifier

264199 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate By similarity. HAMAP MF_00384
Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential HAMAP MF_00384.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW homoserine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	Homoserine kinase HAMAP MF_00384		PRO_1000049179
Regions
	Nucleotide binding	78 – 88	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5M5K5 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 5B41B0586764222B	FASTA	286	30,219
	10 20 30 40 50 60 MKITVPATSA NIGPGFDSVG VAVSKYLTIE VLEPADVWFI EHDLGDIPSD ENNLLISTAL 70 80 90 100 110 120 QVKSDLQPHK LVMASDIPLA RGLGSSSSVI VAGIELANQL ADLKLSDDDK LDIATKIEGH 130 140 150 160 170 180 PDNVAPAIFG NLVVASYVDE HVNSIVTEFP ECAFVAFIPS YELKTSESRG VLPSDLSYKD 190 200 210 220 230 240 AVAASSIANV AIAALFAGDL VKAGRAIQGD MFHERYRQKL VKEFVTIKEL SGQYGAYATY 250 260 270 280 LSGAGPTVMT LTPNDQAEAL KTAIDGIGLD GETLILSVDK GGVVVD

References

[1]

"Complete sequence and comparative genome analysis of the dairy bacterium Streptococcus thermophilus." Bolotin A., Quinquis B., Renault P., Sorokin A., Ehrlich S.D., Kulakauskas S., Lapidus A., Goltsman E., Mazur M., Pusch G.D., Fonstein M., Overbeek R., Kyprides N., Purnelle B., Prozzi D., Ngui K., Masuy D., Hancy F. , Burteau S., Boutry M., Delcour J., Goffeau A., Hols P. Nat. Biotechnol. 22:1554-1558(2004) [PubMed: 15543133] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-250 / LMG 18311.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000023 Genomic DNA. Translation: AAV60180.1.
RefSeq	YP_138995.1. NC_006448.1.
3D structure databases
ProteinModelPortal	Q5M5K5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5M5K5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000058383; EBSTRP00000054426; EBSTRG00000058373.
GeneID	3165224.
GenomeReviews	Gene locus stu0470 in contig CP000023_GR.
KEGG	stl:stu0470.
PATRIC	19802526. VBIStrThe97850_0473.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0083.
GeneTree	EBGT00050000027810.
HOGENOM	HBG646290.
OMA	NNALMST.
PhylomeDB	Q5M5K5.
ProtClustDB	PRK01212.
Enzyme and pathway databases
BioCyc	STHE264199:STU0470-MONOMER.
Family and domain databases
HAMAP	MF_00384. Homoser_kinase. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR006203. GHMP_knse_ATP-bd_CS. IPR000870. Homoserine_kinase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00872.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00191. ThrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_STRT2

Accession

Primary (citable) accession number: Q5M5K5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 1, 2005
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program