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Protein

Glutathione biosynthesis bifunctional protein GshAB

Gene

gshAB

Organism
Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine.UniRule annotation

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.UniRule annotation
ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi: glutathione biosynthesis

This protein is involved in step 1 and 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutathione biosynthesis bifunctional protein GshAB (gshAB)
  2. Glutathione biosynthesis bifunctional protein GshAB (gshAB)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi695Magnesium or manganese 1UniRule annotation1
Metal bindingi716Magnesium or manganese 1UniRule annotation1
Metal bindingi716Magnesium or manganese 2UniRule annotation1
Metal bindingi718Magnesium or manganese 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi515 – 573ATPUniRule annotationAdd BLAST59

GO - Molecular functioni

Keywordsi

Molecular functionLigase, Multifunctional enzyme
Biological processGlutathione biosynthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSTHE264199:G1G41-1337-MONOMER
UniPathwayiUPA00142; UER00209
UPA00142; UER00210

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione biosynthesis bifunctional protein GshABUniRule annotation
Alternative name(s):
Gamma-GCS-GSUniRule annotation
Short name:
GCS-GSUniRule annotation
Including the following 2 domains:
Glutamate--cysteine ligaseUniRule annotation (EC:6.3.2.2UniRule annotation)
Alternative name(s):
Gamma-ECSUniRule annotation
Short name:
GCSUniRule annotation
Gamma-glutamylcysteine synthetaseUniRule annotation
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Alternative name(s):
GSH synthetaseUniRule annotation
Short name:
GSUniRule annotation
Short name:
GSH-SUniRule annotation
Short name:
GSHaseUniRule annotation
Glutathione synthaseUniRule annotation
Gene namesi
Name:gshABUniRule annotation
Synonyms:gshFUniRule annotation
Ordered Locus Names:stu1413
OrganismiStreptococcus thermophilus (strain ATCC BAA-250 / LMG 18311)
Taxonomic identifieri264199 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000001170 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001925621 – 754Glutathione biosynthesis bifunctional protein GshABAdd BLAST754

Proteomic databases

PRIDEiQ5M3J8

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi264199.stu1413

Structurei

3D structure databases

ProteinModelPortaliQ5M3J8
SMRiQ5M3J8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini488 – 746ATP-graspUniRule annotationAdd BLAST259

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 332Glutamate--cysteine ligaseAdd BLAST332

Sequence similaritiesi

In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D9M Bacteria
COG1181 LUCA
COG2918 LUCA
HOGENOMiHOG000156471
KOiK01919
OMAiEANFNPM

Family and domain databases

HAMAPiMF_00782 Glut_biosynth, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR003806 ATP-grasp_DUF201-type
IPR014746 Gln_synth/guanido_kin_cat_dom
IPR007370 Glu_cys_ligase
IPR006335 Glut_biosynth
IPR006334 Glut_cys_ligase
PANTHERiPTHR38761 PTHR38761, 2 hits
PfamiView protein in Pfam
PF02655 ATP-grasp_3, 1 hit
PF04262 Glu_cys_ligase, 2 hits
SUPFAMiSSF55931 SSF55931, 1 hit
TIGRFAMsiTIGR01435 glu_cys_lig_rel, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit

Sequencei

Sequence statusi: Complete.

Q5M3J8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLNQLLQKL EATSPILQAN FGIERESLRV DRQGQLVHTP HPSCLGARSF
60 70 80 90 100
HPYIQTDFCE FQMELITPVA KSTTEARRFL GAITDVAGRS IATDEVLWPL
110 120 130 140 150
SMPPRLKAEE IQVAQLENDF ERHYRNYLAE KYGTKLQAIS GIHYNMELGK
160 170 180 190 200
DLVEALFQES DQTDMIAFKN ALYLKLAQNY LRYRWVITYL FGASPIAEQG
210 220 230 240 250
FFDQEVPEPM RSFRNSDHGY VNKEEIQVSF VSLEDYVSAI ETYIEQGDLI
260 270 280 290 300
AEKEFYSAVR FRGQKVNRSF LDKGITYLEF RNFDLNPFER IGISQTTMDT
310 320 330 340 350
VHLLILAFLW LDSPENVDQA LAQGHALNEK IALSHPLEPL PSEAKTQDIV
360 370 380 390 400
TALDQLVQHF GLGDYHQDLV KQVKAAFADP NQTLSAQLLP YIKDKSLAEF
410 420 430 440 450
ALNKALAYHD YDWTAHYALK GYEEMELSTQ MLLFDAIQKG IHFEILDEQD
460 470 480 490 500
QFLKLWHQDH VEYVKNGNMT SKDNYVVPLA MANKTVTKKI LADASFPVPS
510 520 530 540 550
GDEFTSLEEG LAYYPLIKDK QIVVKPKSTN FGLGISIFQE PASLDNYQKA
560 570 580 590 600
LEIAFAEDTS VLVEEFIPGT EYRFFILDGR CEAVLLRVAA NVIGDGKHTI
610 620 630 640 650
RELVAQKNAN PLRGRDHRSP LEIIELGDIE QLMLAQQGYT PDDILPEGKK
660 670 680 690 700
VNLRRNSNIS TGGDSIDVTE TMDSSYQELA AAMATSMGAW ACGVDLIIPD
710 720 730 740 750
ETQIATKENP HCTCIELNFN PSMYMHTYCA EGPGQAITTK ILDKLFPEIV

AGQT
Length:754
Mass (Da):85,167
Last modified:February 1, 2005 - v1
Checksum:i5D0856B57EDFA2EF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000023 Genomic DNA Translation: AAV61026.1
RefSeqiWP_011226281.1, NC_006448.1

Genome annotation databases

EnsemblBacteriaiAAV61026; AAV61026; stu1413
KEGGistl:stu1413
PATRICifig|264199.4.peg.1388

Similar proteinsi

Entry informationi

Entry nameiGSHAB_STRT2
AccessioniPrimary (citable) accession number: Q5M3J8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: February 1, 2005
Last modified: May 23, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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