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Protein

NAD kinase

Gene

nadK

Organism
Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei57 – 571Proton acceptorUniRule annotation
Binding sitei159 – 1591NADUniRule annotation
Binding sitei161 – 1611NADUniRule annotation
Binding sitei196 – 1961NAD; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi57 – 582NADUniRule annotation
Nucleotide bindingi133 – 1342NADUniRule annotation
Nucleotide bindingi172 – 1776NADUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD+ kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciSTHE264199:GI6K-1410-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:stu1457
OrganismiStreptococcus thermophilus (strain ATCC BAA-250 / LMG 18311)
Taxonomic identifieri264199 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000001170 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 279279NAD kinasePRO_0000229696Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi264199.stu1457.

Structurei

3D structure databases

ProteinModelPortaliQ5M3G7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000275803.
KOiK00858.
OMAiLEIHKKS.
OrthoDBiEOG6PZXDR.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5M3G7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMTQMKTSSD EMKVAIIANG KPQSRRVASK LFNAFRDDPD FYLTKKNPDV
60 70 80 90 100
LISIGGDGML LSAFHMYEKE LARVRFVGIH TGHLGFYTDY LDSEVDQLIE
110 120 130 140 150
TLRKDSGAKI SYPLLNVKLT LADGRSFTSI ALNEAAIKRN EKTMAADVCL
160 170 180 190 200
NDVLFESFRG DGLSVSTPTG STAYNKSLGG AVLHPTIEAL QLTEIASLNN
210 220 230 240 250
RVYRTLGSPL IVPKHEKITV YPTRMGSYTL SVDNKTYINR NVKKVEFSID
260 270
QRKISFVASA SHTSFWERVR ESFIGDMEE
Length:279
Mass (Da):31,167
Last modified:February 1, 2005 - v1
Checksum:i6D23D4684D125FE6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000023 Genomic DNA. Translation: AAV61068.1.
RefSeqiYP_139883.1. NC_006448.1.

Genome annotation databases

EnsemblBacteriaiAAV61068; AAV61068; stu1457.
KEGGistl:stu1457.
PATRICi19804460. VBIStrThe97850_1429.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000023 Genomic DNA. Translation: AAV61068.1.
RefSeqiYP_139883.1. NC_006448.1.

3D structure databases

ProteinModelPortaliQ5M3G7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi264199.stu1457.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAV61068; AAV61068; stu1457.
KEGGistl:stu1457.
PATRICi19804460. VBIStrThe97850_1429.

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000275803.
KOiK00858.
OMAiLEIHKKS.
OrthoDBiEOG6PZXDR.

Enzyme and pathway databases

BioCyciSTHE264199:GI6K-1410-MONOMER.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-250 / LMG 18311.

Entry informationi

Entry nameiNADK_STRT2
AccessioniPrimary (citable) accession number: Q5M3G7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: February 1, 2005
Last modified: April 1, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.