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Protein

Hypoxanthine-guanine phosphoribosyltransferase

Gene

hpt

Organism
Streptococcus thermophilus (strain CNRZ 1066)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein.By similarity

Pathway: IMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes IMP from hypoxanthine.
Proteins known to be involved in this subpathway in this organism are:
  1. Hypoxanthine-guanine phosphoribosyltransferase (hpt)
This subpathway is part of the pathway IMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes IMP from hypoxanthine, the pathway IMP biosynthesis via salvage pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei103 – 1031Proton acceptorBy similarity
Binding sitei131 – 1311IMPBy similarity
Metal bindingi159 – 1591MagnesiumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi99 – 10810IMPBy similarity
Nucleotide bindingi158 – 1592IMPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSTHE299768:GHWB-11-MONOMER.
UniPathwayiUPA00591; UER00648.

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HGPRT
Short name:
HGPRTase
Gene namesi
Name:hpt
Ordered Locus Names:str0011
OrganismiStreptococcus thermophilus (strain CNRZ 1066)
Taxonomic identifieri299768 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 180180Hypoxanthine-guanine phosphoribosyltransferasePRO_0000139629Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ5M216.
SMRiQ5M216. Positions 4-178.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0634.
HOGENOMiHOG000236520.
KOiK00760.
OMAiTMDWMAV.
OrthoDBiEOG693GNP.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5M216-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLEKDIKKIL FSQEDIVTKT KELGQQLTKD YAGKNPLLVC VLKGAVPFMA
60 70 80 90 100
ELIKHIDTHI EMDFMVVSSY GGGTVSSGEV KILKDVDTNI EGRDVIFIED
110 120 130 140 150
IIDTGRTLLY LRDMFKYRKA SSVKIATLFD KPEGRVVDIE ADYVCYDVPN
160 170 180
EFIVGFGLDY DEKYRNLPYV GVLKEEIYNK
Length:180
Mass (Da):20,506
Last modified:February 1, 2005 - v1
Checksum:i196F1F3AF34815AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000024 Genomic DNA. Translation: AAV61630.1.
RefSeqiWP_002948598.1. NC_006449.1.
YP_140445.1. NC_006449.1.

Genome annotation databases

EnsemblBacteriaiAAV61630; AAV61630; str0011.
GeneIDi3163932.
KEGGistc:str0011.
PATRICi19792957. VBIStrThe135799_0012.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000024 Genomic DNA. Translation: AAV61630.1.
RefSeqiWP_002948598.1. NC_006449.1.
YP_140445.1. NC_006449.1.

3D structure databases

ProteinModelPortaliQ5M216.
SMRiQ5M216. Positions 4-178.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAV61630; AAV61630; str0011.
GeneIDi3163932.
KEGGistc:str0011.
PATRICi19792957. VBIStrThe135799_0012.

Phylogenomic databases

eggNOGiCOG0634.
HOGENOMiHOG000236520.
KOiK00760.
OMAiTMDWMAV.
OrthoDBiEOG693GNP.

Enzyme and pathway databases

UniPathwayiUPA00591; UER00648.
BioCyciSTHE299768:GHWB-11-MONOMER.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CNRZ 1066.

Entry informationi

Entry nameiHPRT_STRT1
AccessioniPrimary (citable) accession number: Q5M216
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: February 1, 2005
Last modified: June 24, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.