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Protein

Thymidylate synthase

Gene

thyA

Organism
Streptococcus thermophilus (strain CNRZ 1066)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei161NucleophileUniRule annotation1
Binding sitei1885,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei196dUMPUniRule annotation1
Binding sitei2855,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi140 – 141dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi185 – 188dUMPUniRule annotation4
Nucleotide bindingi226 – 228dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:str0578
OrganismiStreptococcus thermophilus (strain CNRZ 1066)
Taxonomic identifieri299768 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001410361 – 286Thymidylate synthaseAdd BLAST286

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ5M0S7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000257898.
KOiK00560.
OMAiNEWEVGQ.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5M0S7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKADTIFKE NITKIMEEGV WSEQARPKYK DGTTANSKYI TGSFAEYDLS
60 70 80 90 100
KGEFPITTLR PIAIKSAIKE VFWIYQDQTN SLDVLEDKYN VHYWNDWEVE
110 120 130 140 150
GVPANNGDKR SIGQRYGAVV KKHDIINRLL AQLEANPWNR RNVISLWDYE
160 170 180 190 200
AFEETAGLQP CAFQTMFDVR RVGEDVYLDA TLTQRSNDML VAHHINAMQY
210 220 230 240 250
VALQMMIAKH FGWKIGKFFY FINNLHIYDN QFEQAEELLK RQPSDCQPRL
260 270 280
VLNVPDETNF FDIKPEDFEL VDYDPVKPQL KFDLAI
Length:286
Mass (Da):33,384
Last modified:February 1, 2005 - v1
Checksum:i0A1C02A71FF80682
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000024 Genomic DNA. Translation: AAV62174.1.
RefSeqiWP_011226990.1. NC_006449.1.

Genome annotation databases

EnsemblBacteriaiAAV62174; AAV62174; str0578.
KEGGistc:str0578.
PATRICi19794241. VBIStrThe135799_0585.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000024 Genomic DNA. Translation: AAV62174.1.
RefSeqiWP_011226990.1. NC_006449.1.

3D structure databases

ProteinModelPortaliQ5M0S7.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAV62174; AAV62174; str0578.
KEGGistc:str0578.
PATRICi19794241. VBIStrThe135799_0585.

Phylogenomic databases

HOGENOMiHOG000257898.
KOiK00560.
OMAiNEWEVGQ.

Enzyme and pathway databases

UniPathwayiUPA00575.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTYSY_STRT1
AccessioniPrimary (citable) accession number: Q5M0S7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: February 1, 2005
Last modified: November 30, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.