SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q5M0C5

- SYI_STRT1

UniProt

Q5M0C5 - SYI_STRT1

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Isoleucine--tRNA ligase

Gene
ileS, str0741
Organism
Streptococcus thermophilus (strain CNRZ 1066)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei554 – 5541Aminoacyl-adenylate By similarity
Binding sitei598 – 5981ATP By similarity
Metal bindingi888 – 8881Zinc By similarity
Metal bindingi891 – 8911Zinc By similarity
Metal bindingi908 – 9081Zinc By similarity
Metal bindingi911 – 9111Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSTHE299768:GHWB-784-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:str0741
OrganismiStreptococcus thermophilus (strain CNRZ 1066)
Taxonomic identifieri299768 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000001008: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 929929Isoleucine--tRNA ligaseUniRule annotationPRO_0000098487Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi299768.str0741.

Structurei

3D structure databases

ProteinModelPortaliQ5M0C5.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotationAdd
BLAST
Motifi595 – 5995"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5M0C5-1 [UniParc]FASTAAdd to Basket

« Hide

MKLKETLNLG KTSFPMRAGL PNKEPIWQKE WEEAKVYQRR QELNQGKPHF    50
TLHDGPPYAN GNIHVGHAMN KISKDIIVRS KSMSGFYAPF IPGWDTHGLP 100
IEQVLAKQGV KRKELDRAEY LKMCRDYALS QVDKQREDFK RLGVSADWEN 150
PYVTLTPDYE AAQIRVFGEM AKKGYIYQGA KPVYWSWSSE SALAEAEIEY 200
HDLVSTSLYY ANKVKDGKGV LDTDTYIVVW TTTPFTVTAS RGLTVGADIE 250
YVLVKPAGET RKFIVASELL NSLSEKFAWE EVEVLNTYRG DELNQIVTEH 300
PWDSEVDELV ILGEHVTTDS GTGIVHTAPG FGEDDYNVGV ANGLEVVVTV 350
NERGIMMENA GPDFAGKFYD KVAPIVMEKL GDLLLAKEEI SHSYPFDWRT 400
KKPIIWRAVP QWFASVSKFR QEILDEIEKV KFHSEWGKVR LYNMIRDRGD 450
WVISRQRAWG VPLPIFYAED RTPIMTEETI EHVAKLFEEH GSVIWWERDA 500
KDLLPEGFTH PGSPNGEFTK ENDIMDVWFD SGSSWNGVVV NRPELTYPAD 550
LYLEGSDQYR GWFNSSLITS VANHGVAPYK QLLSQGFALD GKGEKMSKSL 600
GNTIAPSDVE KQFGAEILRL WVTSVDTSND VRISMDILSQ VSESYRKIRN 650
TLRFLIANTS DFNPTTDAVA FEDLRSVDQY MTIRFNQLVK NIRDAYENFE 700
FLTIYKSLVN FINVELSAFY LDFAKDVVYI ESAKSLERRQ MQTVFYDILV 750
KITKLLTPIL PHTAEEIWSY LEFENEDYVQ LSELPEAEDF ANQDALLEKW 800
NAFMDFRGKA QKALEEARNE KVIGKSLEAH LTIYPDAEVK ELLESLNTNL 850
AQLLIVSALT IAEGDVPESA VRFQGVSFTV ERAEGEVCDR CRRIDPTTKE 900
RSYNATICNH CASIIEENFA EVVAEGFEV 929
Length:929
Mass (Da):105,854
Last modified:February 1, 2005 - v1
Checksum:iD769FF69E43E4EA8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000024 Genomic DNA. Translation: AAV62334.1.
RefSeqiYP_141149.1. NC_006449.1.

Genome annotation databases

EnsemblBacteriaiAAV62334; AAV62334; str0741.
GeneIDi3166235.
KEGGistc:str0741.
PATRICi19794590. VBIStrThe135799_0755.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000024 Genomic DNA. Translation: AAV62334.1 .
RefSeqi YP_141149.1. NC_006449.1.

3D structure databases

ProteinModelPortali Q5M0C5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 299768.str0741.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAV62334 ; AAV62334 ; str0741 .
GeneIDi 3166235.
KEGGi stc:str0741.
PATRICi 19794590. VBIStrThe135799_0755.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci STHE299768:GHWB-784-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CNRZ 1066.

Entry informationi

Entry nameiSYI_STRT1
AccessioniPrimary (citable) accession number: Q5M0C5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: February 1, 2005
Last modified: May 14, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi