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Q5LYY5

- GSHAB_STRT1

UniProt

Q5LYY5 - GSHAB_STRT1

Protein

Glutathione biosynthesis bifunctional protein GshAB

Gene

gshAB

Organism
Streptococcus thermophilus (strain CNRZ 1066)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (01 Feb 2005)
      Previous versions | rss
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    Functioni

    Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine.UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.UniRule annotation
    ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi695 – 6951Magnesium or manganese 1UniRule annotation
    Metal bindingi716 – 7161Magnesium or manganese 1UniRule annotation
    Metal bindingi716 – 7161Magnesium or manganese 2UniRule annotation
    Metal bindingi718 – 7181Magnesium or manganese 2UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi515 – 57359ATPUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-cysteine ligase activity Source: UniProtKB-HAMAP
    3. glutathione synthase activity Source: UniProtKB-HAMAP
    4. magnesium ion binding Source: UniProtKB-HAMAP
    5. manganese ion binding Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Glutathione biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSTHE299768:GHWB-1401-MONOMER.
    UniPathwayiUPA00142; UER00209.
    UPA00142; UER00210.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione biosynthesis bifunctional protein GshABUniRule annotation
    Alternative name(s):
    Gamma-GCS-GSUniRule annotation
    Short name:
    GCS-GSUniRule annotation
    Including the following 2 domains:
    Glutamate--cysteine ligaseUniRule annotation (EC:6.3.2.2UniRule annotation)
    Alternative name(s):
    Gamma-ECSUniRule annotation
    Short name:
    GCSUniRule annotation
    Gamma-glutamylcysteine synthetaseUniRule annotation
    Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
    Alternative name(s):
    GSH synthetaseUniRule annotation
    Short name:
    GSUniRule annotation
    Short name:
    GSH-SUniRule annotation
    Short name:
    GSHaseUniRule annotation
    Glutathione synthaseUniRule annotation
    Gene namesi
    Name:gshABUniRule annotation
    Synonyms:gshFUniRule annotation
    Ordered Locus Names:str1413
    OrganismiStreptococcus thermophilus (strain CNRZ 1066)
    Taxonomic identifieri299768 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
    ProteomesiUP000001008: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 754754Glutathione biosynthesis bifunctional protein GshABPRO_0000192561Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi299768.str1413.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5LYY5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini488 – 746259ATP-graspUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 332332Glutamate--cysteine ligaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily.UniRule annotation
    Contains 1 ATP-grasp domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1181.
    HOGENOMiHOG000156471.
    KOiK01919.
    OMAiHVEYVKN.
    OrthoDBiEOG6BKJ7H.

    Family and domain databases

    Gene3Di3.30.470.20. 1 hit.
    HAMAPiMF_00782. Glut_biosynth.
    InterProiIPR011761. ATP-grasp.
    IPR013816. ATP_grasp_subdomain_2.
    IPR007370. Glu_cys_ligase.
    IPR006335. Glut_biosynth.
    [Graphical view]
    PfamiPF04262. Glu_cys_ligase. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01435. glu_cys_lig_rel. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5LYY5-1 [UniParc]FASTAAdd to Basket

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    MTLNQLLQKL EATSPILQAN FGIERESLRV DRQGQLVHTP HPSCLGARSF    50
    HPYIQTDFCE FQMELITPVA KSTTEARRFL GAITDVAGRS IATDEVLWPL 100
    SMPPRLKAEE IQVAQLENDF ERHYRNYLAE KYGTKLQAIS GIHYNMELGK 150
    DLVEALFQES DQTDMIAFKN ALYLKLAQNY LRYRWVITYL FGASPIAEQG 200
    FFDQEVPEPM RSFRNSDHGY VNKEEIQVSF VSLEDYVSAI ETYIEQGDLI 250
    AEKEFYSAVR FRGQKVNRSF LDKGITYLEF RNFDLNPFER IGISQTTMDT 300
    VHLLILAFLW LDSPENVDQA LAQGHALNEK IALSHPLEPL PSEAKTQDIV 350
    TALDQLVQHF GLGDYHQDLV KQVKAAFADP NQTLSAQLLP YIKDKSLAEF 400
    ALNKALAYHD YDWTAHYALK GYEEMELSTQ MLLFDAIQKG IHFEILDEQD 450
    QFLKLWHQDH VEYVKNGNMT SKDNYVVPLA MANKTVTKKI LADAGFPVPS 500
    GDEFTSLEEG LAYYPLIKDK QIVVKPKSTN FGLGISIFQE PASLDNYQKA 550
    LEIAFAEDTS VLVEEFIPGT EYRFFILDGR CEAVLLRVAA NVIGDGKHTI 600
    RELVAQKNAN PLRGRDHRSP LEIIELGDIE QLMLAQQGYT PDDILPEGKK 650
    VNLRRNSNIS TGGDSIDVTE TMDSSYQELA AAMATSMGAW ACGVDLIIPD 700
    ETQIATKENP HCTCIELNFN PSMYMHTYCA EGPGQAITTK ILDKLFPEIV 750
    AGQT 754
    Length:754
    Mass (Da):85,137
    Last modified:February 1, 2005 - v1
    Checksum:i83C856B2641FA2E3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000024 Genomic DNA. Translation: AAV62950.1.
    RefSeqiYP_141765.1. NC_006449.1.

    Genome annotation databases

    EnsemblBacteriaiAAV62950; AAV62950; str1413.
    GeneIDi3167482.
    KEGGistc:str1413.
    PATRICi19795897. VBIStrThe135799_1404.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000024 Genomic DNA. Translation: AAV62950.1 .
    RefSeqi YP_141765.1. NC_006449.1.

    3D structure databases

    ProteinModelPortali Q5LYY5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 299768.str1413.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAV62950 ; AAV62950 ; str1413 .
    GeneIDi 3167482.
    KEGGi stc:str1413.
    PATRICi 19795897. VBIStrThe135799_1404.

    Phylogenomic databases

    eggNOGi COG1181.
    HOGENOMi HOG000156471.
    KOi K01919.
    OMAi HVEYVKN.
    OrthoDBi EOG6BKJ7H.

    Enzyme and pathway databases

    UniPathwayi UPA00142 ; UER00209 .
    UPA00142 ; UER00210 .
    BioCyci STHE299768:GHWB-1401-MONOMER.

    Family and domain databases

    Gene3Di 3.30.470.20. 1 hit.
    HAMAPi MF_00782. Glut_biosynth.
    InterProi IPR011761. ATP-grasp.
    IPR013816. ATP_grasp_subdomain_2.
    IPR007370. Glu_cys_ligase.
    IPR006335. Glut_biosynth.
    [Graphical view ]
    Pfami PF04262. Glu_cys_ligase. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01435. glu_cys_lig_rel. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CNRZ 1066.

    Entry informationi

    Entry nameiGSHAB_STRT1
    AccessioniPrimary (citable) accession number: Q5LYY5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 5, 2005
    Last sequence update: February 1, 2005
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3