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Protein

Glutathione biosynthesis bifunctional protein GshAB

Gene

gshAB

Organism
Streptococcus thermophilus (strain CNRZ 1066)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine.UniRule annotation

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.UniRule annotation
ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathway: glutathione biosynthesis

This protein is involved in step 1 and 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutathione biosynthesis bifunctional protein GshAB (gshAB)
  2. Glutathione biosynthesis bifunctional protein GshAB (gshAB)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi695 – 6951Magnesium or manganese 1UniRule annotation
Metal bindingi716 – 7161Magnesium or manganese 1UniRule annotation
Metal bindingi716 – 7161Magnesium or manganese 2UniRule annotation
Metal bindingi718 – 7181Magnesium or manganese 2UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi515 – 57359ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSTHE299768:GHWB-1401-MONOMER.
UniPathwayiUPA00142; UER00209.
UPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione biosynthesis bifunctional protein GshABUniRule annotation
Alternative name(s):
Gamma-GCS-GSUniRule annotation
Short name:
GCS-GSUniRule annotation
Including the following 2 domains:
Glutamate--cysteine ligaseUniRule annotation (EC:6.3.2.2UniRule annotation)
Alternative name(s):
Gamma-ECSUniRule annotation
Short name:
GCSUniRule annotation
Gamma-glutamylcysteine synthetaseUniRule annotation
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Alternative name(s):
GSH synthetaseUniRule annotation
Short name:
GSUniRule annotation
Short name:
GSH-SUniRule annotation
Short name:
GSHaseUniRule annotation
Glutathione synthaseUniRule annotation
Gene namesi
Name:gshABUniRule annotation
Synonyms:gshFUniRule annotation
Ordered Locus Names:str1413
OrganismiStreptococcus thermophilus (strain CNRZ 1066)
Taxonomic identifieri299768 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 754754Glutathione biosynthesis bifunctional protein GshABPRO_0000192561Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ5LYY5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini488 – 746259ATP-graspUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 332332Glutamate--cysteine ligaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1181.
HOGENOMiHOG000156471.
KOiK01919.
OMAiFNPSMYM.
OrthoDBiEOG6BKJ7H.

Family and domain databases

Gene3Di3.30.470.20. 1 hit.
HAMAPiMF_00782. Glut_biosynth.
InterProiIPR011761. ATP-grasp.
IPR013816. ATP_grasp_subdomain_2.
IPR007370. Glu_cys_ligase.
IPR006335. Glut_biosynth.
[Graphical view]
PfamiPF04262. Glu_cys_ligase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01435. glu_cys_lig_rel. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5LYY5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLNQLLQKL EATSPILQAN FGIERESLRV DRQGQLVHTP HPSCLGARSF
60 70 80 90 100
HPYIQTDFCE FQMELITPVA KSTTEARRFL GAITDVAGRS IATDEVLWPL
110 120 130 140 150
SMPPRLKAEE IQVAQLENDF ERHYRNYLAE KYGTKLQAIS GIHYNMELGK
160 170 180 190 200
DLVEALFQES DQTDMIAFKN ALYLKLAQNY LRYRWVITYL FGASPIAEQG
210 220 230 240 250
FFDQEVPEPM RSFRNSDHGY VNKEEIQVSF VSLEDYVSAI ETYIEQGDLI
260 270 280 290 300
AEKEFYSAVR FRGQKVNRSF LDKGITYLEF RNFDLNPFER IGISQTTMDT
310 320 330 340 350
VHLLILAFLW LDSPENVDQA LAQGHALNEK IALSHPLEPL PSEAKTQDIV
360 370 380 390 400
TALDQLVQHF GLGDYHQDLV KQVKAAFADP NQTLSAQLLP YIKDKSLAEF
410 420 430 440 450
ALNKALAYHD YDWTAHYALK GYEEMELSTQ MLLFDAIQKG IHFEILDEQD
460 470 480 490 500
QFLKLWHQDH VEYVKNGNMT SKDNYVVPLA MANKTVTKKI LADAGFPVPS
510 520 530 540 550
GDEFTSLEEG LAYYPLIKDK QIVVKPKSTN FGLGISIFQE PASLDNYQKA
560 570 580 590 600
LEIAFAEDTS VLVEEFIPGT EYRFFILDGR CEAVLLRVAA NVIGDGKHTI
610 620 630 640 650
RELVAQKNAN PLRGRDHRSP LEIIELGDIE QLMLAQQGYT PDDILPEGKK
660 670 680 690 700
VNLRRNSNIS TGGDSIDVTE TMDSSYQELA AAMATSMGAW ACGVDLIIPD
710 720 730 740 750
ETQIATKENP HCTCIELNFN PSMYMHTYCA EGPGQAITTK ILDKLFPEIV

AGQT
Length:754
Mass (Da):85,137
Last modified:February 1, 2005 - v1
Checksum:i83C856B2641FA2E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000024 Genomic DNA. Translation: AAV62950.1.
RefSeqiWP_011227399.1. NC_006449.1.
YP_141765.1. NC_006449.1.

Genome annotation databases

EnsemblBacteriaiAAV62950; AAV62950; str1413.
KEGGistc:str1413.
PATRICi19795897. VBIStrThe135799_1404.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000024 Genomic DNA. Translation: AAV62950.1.
RefSeqiWP_011227399.1. NC_006449.1.
YP_141765.1. NC_006449.1.

3D structure databases

ProteinModelPortaliQ5LYY5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAV62950; AAV62950; str1413.
KEGGistc:str1413.
PATRICi19795897. VBIStrThe135799_1404.

Phylogenomic databases

eggNOGiCOG1181.
HOGENOMiHOG000156471.
KOiK01919.
OMAiFNPSMYM.
OrthoDBiEOG6BKJ7H.

Enzyme and pathway databases

UniPathwayiUPA00142; UER00209.
UPA00142; UER00210.
BioCyciSTHE299768:GHWB-1401-MONOMER.

Family and domain databases

Gene3Di3.30.470.20. 1 hit.
HAMAPiMF_00782. Glut_biosynth.
InterProiIPR011761. ATP-grasp.
IPR013816. ATP_grasp_subdomain_2.
IPR007370. Glu_cys_ligase.
IPR006335. Glut_biosynth.
[Graphical view]
PfamiPF04262. Glu_cys_ligase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01435. glu_cys_lig_rel. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CNRZ 1066.

Entry informationi

Entry nameiGSHAB_STRT1
AccessioniPrimary (citable) accession number: Q5LYY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: February 1, 2005
Last modified: June 24, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.