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Q5LYY5

- GSHAB_STRT1

UniProt

Q5LYY5 - GSHAB_STRT1

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Protein

Glutathione biosynthesis bifunctional protein GshAB

Gene
gshAB, gshF, str1413
Organism
Streptococcus thermophilus (strain CNRZ 1066)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine By similarity.UniRule annotation

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.UniRule annotation
ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi695 – 6951Magnesium or manganese 1 By similarity
Metal bindingi716 – 7161Magnesium or manganese 1 By similarity
Metal bindingi716 – 7161Magnesium or manganese 2 By similarity
Metal bindingi718 – 7181Magnesium or manganese 2 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi515 – 57359ATP By similarityAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-cysteine ligase activity Source: UniProtKB-HAMAP
  3. glutathione synthase activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP
  5. manganese ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSTHE299768:GHWB-1401-MONOMER.
UniPathwayiUPA00142; UER00209.
UPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione biosynthesis bifunctional protein GshAB
Alternative name(s):
Gamma-GCS-GS
Short name:
GCS-GS
Including the following 2 domains:
Glutamate--cysteine ligase (EC:6.3.2.2)
Alternative name(s):
Gamma-ECS
Short name:
GCS
Gamma-glutamylcysteine synthetase
Glutathione synthetase (EC:6.3.2.3)
Alternative name(s):
GSH synthetase
Short name:
GS
Short name:
GSH-S
Short name:
GSHase
Glutathione synthase
Gene namesi
Name:gshAB
Synonyms:gshF
Ordered Locus Names:str1413
OrganismiStreptococcus thermophilus (strain CNRZ 1066)
Taxonomic identifieri299768 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000001008: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 754754Glutathione biosynthesis bifunctional protein GshABUniRule annotationPRO_0000192561Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi299768.str1413.

Structurei

3D structure databases

ProteinModelPortaliQ5LYY5.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini488 – 746259ATP-graspAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 332332Glutamate--cysteine ligaseUniRule annotationAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily.
Contains 1 ATP-grasp domain.

Phylogenomic databases

eggNOGiCOG1181.
HOGENOMiHOG000156471.
KOiK01919.
OMAiHVEYVKN.
OrthoDBiEOG6BKJ7H.

Family and domain databases

Gene3Di3.30.470.20. 1 hit.
HAMAPiMF_00782. Glut_biosynth.
InterProiIPR011761. ATP-grasp.
IPR013816. ATP_grasp_subdomain_2.
IPR007370. Glu_cys_ligase.
IPR006335. Glut_biosynth.
[Graphical view]
PfamiPF04262. Glu_cys_ligase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01435. glu_cys_lig_rel. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5LYY5-1 [UniParc]FASTAAdd to Basket

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MTLNQLLQKL EATSPILQAN FGIERESLRV DRQGQLVHTP HPSCLGARSF    50
HPYIQTDFCE FQMELITPVA KSTTEARRFL GAITDVAGRS IATDEVLWPL 100
SMPPRLKAEE IQVAQLENDF ERHYRNYLAE KYGTKLQAIS GIHYNMELGK 150
DLVEALFQES DQTDMIAFKN ALYLKLAQNY LRYRWVITYL FGASPIAEQG 200
FFDQEVPEPM RSFRNSDHGY VNKEEIQVSF VSLEDYVSAI ETYIEQGDLI 250
AEKEFYSAVR FRGQKVNRSF LDKGITYLEF RNFDLNPFER IGISQTTMDT 300
VHLLILAFLW LDSPENVDQA LAQGHALNEK IALSHPLEPL PSEAKTQDIV 350
TALDQLVQHF GLGDYHQDLV KQVKAAFADP NQTLSAQLLP YIKDKSLAEF 400
ALNKALAYHD YDWTAHYALK GYEEMELSTQ MLLFDAIQKG IHFEILDEQD 450
QFLKLWHQDH VEYVKNGNMT SKDNYVVPLA MANKTVTKKI LADAGFPVPS 500
GDEFTSLEEG LAYYPLIKDK QIVVKPKSTN FGLGISIFQE PASLDNYQKA 550
LEIAFAEDTS VLVEEFIPGT EYRFFILDGR CEAVLLRVAA NVIGDGKHTI 600
RELVAQKNAN PLRGRDHRSP LEIIELGDIE QLMLAQQGYT PDDILPEGKK 650
VNLRRNSNIS TGGDSIDVTE TMDSSYQELA AAMATSMGAW ACGVDLIIPD 700
ETQIATKENP HCTCIELNFN PSMYMHTYCA EGPGQAITTK ILDKLFPEIV 750
AGQT 754
Length:754
Mass (Da):85,137
Last modified:February 1, 2005 - v1
Checksum:i83C856B2641FA2E3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000024 Genomic DNA. Translation: AAV62950.1.
RefSeqiYP_141765.1. NC_006449.1.

Genome annotation databases

EnsemblBacteriaiAAV62950; AAV62950; str1413.
GeneIDi3167482.
KEGGistc:str1413.
PATRICi19795897. VBIStrThe135799_1404.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000024 Genomic DNA. Translation: AAV62950.1 .
RefSeqi YP_141765.1. NC_006449.1.

3D structure databases

ProteinModelPortali Q5LYY5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 299768.str1413.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAV62950 ; AAV62950 ; str1413 .
GeneIDi 3167482.
KEGGi stc:str1413.
PATRICi 19795897. VBIStrThe135799_1404.

Phylogenomic databases

eggNOGi COG1181.
HOGENOMi HOG000156471.
KOi K01919.
OMAi HVEYVKN.
OrthoDBi EOG6BKJ7H.

Enzyme and pathway databases

UniPathwayi UPA00142 ; UER00209 .
UPA00142 ; UER00210 .
BioCyci STHE299768:GHWB-1401-MONOMER.

Family and domain databases

Gene3Di 3.30.470.20. 1 hit.
HAMAPi MF_00782. Glut_biosynth.
InterProi IPR011761. ATP-grasp.
IPR013816. ATP_grasp_subdomain_2.
IPR007370. Glu_cys_ligase.
IPR006335. Glut_biosynth.
[Graphical view ]
Pfami PF04262. Glu_cys_ligase. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01435. glu_cys_lig_rel. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CNRZ 1066.

Entry informationi

Entry nameiGSHAB_STRT1
AccessioniPrimary (citable) accession number: Q5LYY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: February 1, 2005
Last modified: May 14, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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