Q5LWR1 (GLPK_SILPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	SPO0104

Organism

Silicibacter pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) [Complete proteome] [HAMAP]

Taxonomic identifier

246200 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Ruegeria

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 492	492	Glycerol kinase HAMAP MF_00186		PRO_1000020788
Regions
	Nucleotide binding	407 – 411	5	ATP By similarity
Sites
	Binding site	11	1	Substrate By similarity
	Binding site	15	1	ATP By similarity
	Binding site	81	1	Substrate By similarity
	Binding site	133	1	Substrate By similarity
	Binding site	240	1	Substrate By similarity
	Binding site	262	1	ATP By similarity
	Binding site	305	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5LWR1 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 6DAD0DE44B598658	FASTA	492	53,036
	10 20 30 40 50 60 MTFILAIDQG TTSSRAILFD GEMRRVATAQ REFTQHFPEA GWVEHDAEEI WQTTVEVCRE 70 80 90 100 110 120 VIAGQGIGPE QIAGIGITNQ RETTVVWDKA GGAPVHRAIV WQDRRTAAIC ERLRHAGCED 130 140 150 160 170 180 DFTAQTGLLL DPYFSGTKVK WILDTVPGAR DRAAAGELLF GTIDSFLIWR LTGGRVHASD 190 200 210 220 230 240 ATNAARTLMF DIHQGEWSKD ICELLDVPMS MLPEVRDCAA DFGTTDLFGG QVPILGVAGD 250 260 270 280 290 300 QQAATIGQAC FQPGMMKSTY GTGCFALLNT GTQPVASQNR LLTTIAYQLD GQRTYALEGS 310 320 330 340 350 360 IFIAGAAVQW LRDGLGIIEN AAQSGALAAR ADPAQNVILV PAFTGLGAPY WKPDCRGAMF 370 380 390 400 410 420 GLTRNSGPAE FCKAALESVA YQTRDLWEAM QGDWGADADV ILRVDGGMSA SDWAMQGLAD 430 440 450 460 470 480 RLGAPVDRPV MQETTALGAA WLAGMRAGVY PDQAGFAATW ALDARFTPKM VEGDREAAYR 490 RWKAAVAAAM MV

References

[1]

"Genome sequence of Silicibacter pomeroyi reveals adaptations to the marine environment." Moran M.A., Buchan A., Gonzalez J.M., Heidelberg J.F., Whitman W.B., Kiene R.P., Henriksen J.R., King G.M., Belas R., Fuqua C., Brinkac L.M., Lewis M., Johri S., Weaver B., Pai G., Eisen J.A., Rahe E., Sheldon W.M. , Ye W., Miller T.R., Carlton J., Rasko D.A., Paulsen I.T., Ren Q., Daugherty S.C., DeBoy R.T., Dodson R.J., Durkin A.S., Madupu R., Nelson W.C., Sullivan S.A., Rosovitz M.J., Haft D.H., Selengut J., Ward N. Nature 432:910-913(2004) [PubMed: 15602564] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700808 / DSM 15171 / DSS-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000031 Genomic DNA. Translation: AAV93435.1.
RefSeq	YP_165378.1. NC_003911.11.
3D structure databases
HSSP	HSSP built from PDB template 1GLC based on UniProtKB P0A6F3.
ProteinModelPortal	Q5LWR1.
SMR	Q5LWR1. Positions 3-488.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3194950.
GenomeReviews	Gene locus SPO0104 in contig CP000031_GR.
KEGG	sil:SPO0104.
NMPDR	fig|246200.3.peg.551.
PATRIC	23373445. VBIRuePom114501_0108.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG511469.
OMA	CYQTRDL.
ProtClustDB	CLSK751479.
Enzyme and pathway databases
BioCyc	RPOM246200:SPO_0104-MONOMER.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. False negative. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_SILPO

Accession

Primary (citable) accession number: Q5LWR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 1, 2005
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program