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Q5LWL4 (IF2_RUEPO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Translation initiation factor IF-2
Gene names
Name:infB
Ordered Locus Names:SPO0061
OrganismRuegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter pomeroyi) [Complete proteome] [HAMAP]
Taxonomic identifier246200 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRuegeria

Protein attributes

Sequence length835 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 70S ribosomal complex By similarity. HAMAP-Rule MF_00100

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00100.

Sequence similarities

Belongs to the IF-2 family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandGTP-binding
Nucleotide-binding
   Molecular functionInitiation factor
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

translation initiation factor activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 835835Translation initiation factor IF-2 HAMAP-Rule MF_00100
PRO_0000228244

Regions

Nucleotide binding341 – 3488GTP By similarity
Nucleotide binding388 – 3925GTP By similarity
Nucleotide binding442 – 4454GTP By similarity
Region335 – 484150G-domain HAMAP-Rule MF_00100

Sequences

Sequence LengthMass (Da)Tools
Q5LWL4 [UniParc].

Last modified February 1, 2005. Version 1.
Checksum: 543CC094F33DD84A

FASTA83590,200
        10         20         30         40         50         60 
MSDTDGKKTL GLRGSRPGNV KQSFSHGRTK NVVVETKRKR VVVPKPGAGK PSAGGSSPAG 

        70         80         90        100        110        120 
DPSRRPAGIS DAEMERRLNA LKAAKARESE EAAQREAEEK ARAEERERRR AEQEAKEREQ 

       130        140        150        160        170        180 
REAEQRAREK AEEEERQRRE AEEEAKRAAV RAAAEQEAPK AERSAERAPA AARPEGGDNA 

       190        200        210        220        230        240 
RRTTDRDRER EQRQTRGKGR QDGRRSGKLT LSQVTDGEGG RQKSLAAMKR KQERARQKAM 

       250        260        270        280        290        300 
GGAAEREKVI REVQLPEAIV VSELANRMAE RVADVVKELM KMGMMVTQNQ TIDADTAELI 

       310        320        330        340        350        360 
IEEFGHKVVR VSDSDVEDVI SDIEDAEEDL KPRPPVITIM GHVDHGKTSL LDAIRDAKVV 

       370        380        390        400        410        420 
AGEAGGITQH IGAYQVKTDG GATLSFLDTP GHAAFTSMRS RGAQVTDIVV LVVAADDAVM 

       430        440        450        460        470        480 
PQTVEAINHA KAAGVPMIVA INKIDKPEAN PTKVRTDLLQ HEVVVEAMSG EVQDVEVSAK 

       490        500        510        520        530        540 
TGEGLDELLE AIALQAEILE LKANPDRPAQ GAVIEAQLDV GRGPVATVLI QKGTLRQGDI 

       550        560        570        580        590        600 
FVVGEQYGKV RALINDKGER VSEAGPSVPV EVLGLNGTPE AGDVLNVTST EAQAREIAEY 

       610        620        630        640        650        660 
RAQVAKDKRA AAGAATTLEQ LMAKAKADEN VAELPILVKA DVQGSAEAIV QAMEKIGNDE 

       670        680        690        700        710        720 
VRVRVLHSGV GAITETDVGL AEASGAPIMG FNVRANASAR NTANQKGVEI RYYSVIYDLV 

       730        740        750        760        770        780 
DDVKAAASGL LSAEIRENFI GYANIKEVFK VSNVGKVAGC LVTEGVARRS AGVRLLRDNV 

       790        800        810        820        830 
VIHEGTLKTL KRFKDEVAEV QSGQECGMAF ENYDDIRPGD VIEIFEREEV TRTLT 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000031 Genomic DNA. Translation: AAV93392.1.
RefSeqYP_165335.1. NC_003911.12.

3D structure databases

ProteinModelPortalQ5LWL4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING246200.SPO0061.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAV93392; AAV93392; SPO0061.
GeneID3193823.
KEGGsil:SPO0061.
PATRIC23373359. VBIRuePom114501_0065.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0532.
HOGENOMHOG000076907.
KOK02519.
OMAHKITDVI.
OrthoDBEOG67HJSV.

Family and domain databases

Gene3D3.40.50.10050. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_00100_B. IF_2_B.
InterProIPR000795. EF_GTP-bd_dom.
IPR013575. IF2_assoc_dom_bac.
IPR006847. IF2_N.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR000178. TF_IF2_bacterial-like.
IPR015760. TIF_IF2.
IPR023115. TIF_IF2_dom3.
IPR009000. Transl_B-barrel.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PANTHERPTHR23115:SF41. PTHR23115:SF41. 1 hit.
PfamPF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF11987. IF-2. 1 hit.
PF08364. IF2_assoc. 1 hit.
PF04760. IF2_N. 1 hit.
[Graphical view]
SUPFAMSSF50447. SSF50447. 2 hits.
SSF52156. SSF52156. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00487. IF-2. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS01176. IF2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIF2_RUEPO
AccessionPrimary (citable) accession number: Q5LWL4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: February 1, 2005
Last modified: May 14, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Translation initiation factors

List of translation initiation factor entries