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Q5LWE5

- GLND_RUEPO

UniProt

Q5LWE5 - GLND_RUEPO

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter pomeroyi)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:SPO0397
OrganismiRuegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter pomeroyi)
Taxonomic identifieri246200 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRuegeria
ProteomesiUP000001023: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 908908Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000192769Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi246200.SPO0397.

Structurei

3D structure databases

ProteinModelPortaliQ5LWE5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini478 – 598121HDUniRule annotationAdd
BLAST
Domaini719 – 80183ACT 1UniRule annotationAdd
BLAST
Domaini829 – 90476ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 360360UridylyltransferaseAdd
BLAST
Regioni361 – 718358Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261779.
KOiK00990.
OMAiLYCLWDM.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5LWE5-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MFDTPAVFAR ITEAAAEAAD NAALRGAVVA ILRDVQNAGR AAIAEGFAED
60 70 80 90 100
PFAARPLTRA YTYLTDGMVK TAMYVASEIL HPLATPTQGE RIAVLAVGGF
110 120 130 140 150
GRGEMAPFSD VDLLFLTPYK ITAWAESVIE SMLYILWDLR LKVGHSSRTV
160 170 180 190 200
KDCIRLGRDD FTIRTAMLEH RFLAGHAPLA RSLDQRLKSE LYKDTQREFI
210 220 230 240 250
EAKLEERDAR HRKQGERYMV EPNVKEGKGG LRDLQSLYWI AKYIYEVKET
260 270 280 290 300
AELVPLGLFT PSEYRTFVQA EEFLWAVRAH LHLVTGRATE QLTFDLQVEV
310 320 330 340 350
AARMGYQDRA GRRGVEVFMQ KYFREATRVG ELTRIFLTKL EAAHMKGAPL
360 370 380 390 400
LERIFRRRRR IKQGYKVVRG RLDVVDPEAF LADKLNLLRI FEEALRTGML
410 420 430 440 450
IHPDAMRLVT ANLDLIDDGM RNDKEARRIF LDLLLKHGNP ERALRRMNEL
460 470 480 490 500
GVLSAFVPEF EPIVAMMQFN MYHSYTVDEH TIQTIVNLAQ IEKGELVESL
510 520 530 540 550
PLASSILKAG VNRKVLYVAL LLHDIGKGRP EDHSILGARI ARKVAPRLGL
560 570 580 590 600
SKADCETVEW LVRYHLLMSD MAQKRDISDP RTVRDFAKAV QTTKRLDLLT
610 620 630 640 650
VLTVCDIRGV GPNTWNNWKA TLLRALHAET KRALEMGMEA LNREGRGNEA
660 670 680 690 700
KKALRTALSD WPAGEVKTEI ARHYPPYWQG FNVETHVTFA EMLRQLEHSG
710 720 730 740 750
DPGGIEIRLD PDEDRDATRA CFAMADHPGI FSRMAGALAL VGANVVDARS
760 770 780 790 800
YTTKDGYVTD AFWIQDAEGH PYEAARLPRL SQMILKTLKG EVVARDALKS
810 820 830 840 850
RDKIKKREKA FNVPTHITFD NEGSDIYTII EVDTRDRPGL LYDLARALAA
860 870 880 890 900
ANVYIANAVI ATYGEQVVDS FYVKDMFGLK YHSEAKQRTL ETKLRKAITE

GAERAAAS
Length:908
Mass (Da):102,501
Last modified:February 1, 2005 - v1
Checksum:iAA6C869FD08E01F5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000031 Genomic DNA. Translation: AAV93715.1.
RefSeqiYP_165660.1. NC_003911.12.

Genome annotation databases

EnsemblBacteriaiAAV93715; AAV93715; SPO0397.
GeneIDi3195406.
KEGGisil:SPO0397.
PATRICi23374055. VBIRuePom114501_0404.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000031 Genomic DNA. Translation: AAV93715.1 .
RefSeqi YP_165660.1. NC_003911.12.

3D structure databases

ProteinModelPortali Q5LWE5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 246200.SPO0397.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAV93715 ; AAV93715 ; SPO0397 .
GeneIDi 3195406.
KEGGi sil:SPO0397.
PATRICi 23374055. VBIRuePom114501_0404.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261779.
KOi K00990.
OMAi LYCLWDM.
OrthoDBi EOG6CCH44.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700808 / DSM 15171 / DSS-3.

Entry informationi

Entry nameiGLND_RUEPO
AccessioniPrimary (citable) accession number: Q5LWE5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: February 1, 2005
Last modified: November 26, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3