Reviewed, UniProtKB/Swiss-Prot Q5LVN0 (DNAE2_SILPO)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	SPO0670

Organism

Silicibacter pomeroyi [Complete proteome] [HAMAP]

Taxonomic identifier

89184 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Silicibacter

Protein attributes

Sequence length	1101 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subcellular location	CytoplasmProbable.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: InterPro error-prone DNA repair Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1101	1101	Error-prone DNA polymerase		PRO_0000103399

Sequences

	Sequence		Length	Mass (Da)
	Q5LVN0-1 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 5D732922EEE73E1F	FASTA	1,101	122,743
	10 20 30 40 50 60 MTYAELCVTT NFTFLTGASH PEEMITRAAE LGLHAIAITD RNSLAGVVRA YAALKELRRE 70 80 90 100 110 120 ADEAIRIRSQ HRIDSCSRQE VGHPQPIARP EAPRLPRLIT GCRLVLRDSP LHWIALPRDR 130 140 150 160 170 180 AAYQRLTRLL TLGKRRAEKG DCHLDLADLL AGCTGMILIA LPQGRLDGAL PHLQQVQRRF 190 200 210 220 230 240 PGHVFLGAAP RYDGSDQAWL AACARLALRS SAPMVAVGDA LMHRANRRQL ADVLTCMREH 250 260 270 280 290 300 ITIDRIGTRA LPNAERRLKG HADMARLFRD HPAALRRTLD IAARCSFCLS ELSYEYPDEV 310 320 330 340 350 360 AEGETPQARL ERLTAEGIVR RYPGGAPQRV HELVAKELKL VAELGFPAYF LTVHDIVQFA 370 380 390 400 410 420 RSQGILCQGR GSAANSILCY LLGITDVSPD QIAMVFERFI SKYRGEPPDI DVDFEHERRE 430 440 450 460 470 480 EVIQWIYQRY GRHRAGLCAT VIHFRSRAAI REVGKVMGLS QDVTASLSGQ IWGQSNGGAD 490 500 510 520 530 540 PERMRELGLD PEDRRLALTI RLIGEIIGFP RHLSQHVGGF VITRGRLDEL APIENAAMED 550 560 570 580 590 600 RTLIEWDKDD IDTLGILKVD VLGLGMLTCI RKAFDLMREH EQRHLSIDTV PQEDAATYDM 610 620 630 640 650 660 LCAADAVGVF QVESRAQMNF LPRMRPRTFY DLVIEVAIVR PGPIQGGMVQ PYIRRRQGLE 670 680 690 700 710 720 KAEPFGPELA AVTARTLGVP LFQEQALQIA VVGAGYTAEE ADHLRRSLAS FRRMGTIGAH 730 740 750 760 770 780 RDTFIAGMRR NGYSQEVAER CFGQIEGFAD YGFPESHAAA FAMLAYVSAW LKCHHPAIFA 790 800 810 820 830 840 CALLNAQPMG FYAPAQIVRD ARDHQVELRP ICVNASDWDN RLERRADGAL ALRLGFRQIK 850 860 870 880 890 900 GFREEDAAWI VAARGNGYPD PQALWLRAGV TPAVLERLAE ADAFADMGIG RRDALWQVRA 910 920 930 940 950 960 IRGQAPLPLF NDPLDGEIIH EPQVTLPAMH LGEEVVEDYV SMRLTLRAHP MELLRPAIPG 970 980 990 1000 1010 1020 LTPHDKLASV AAHRVSVCGL VITRQRPGTA SGVIFLTLED ETGVSNVVVW PKIYEQFRRI 1030 1040 1050 1060 1070 1080 VMGGRLLRVT GTLQREGIVV HLIAQRIEDL SPRLADLGHP MDSAVGQTTP QTDSAPRPRP 1090 1100 QPRAMHPREQ AKRLFPSRDF H

References

[1]

"Genome sequence of Silicibacter pomeroyi reveals adaptations to the marine environment." Moran M.A., Buchan A., Gonzalez J.M., Heidelberg J.F., Whitman W.B., Kiene R.P., Henriksen J.R., King G.M., Belas R., Fuqua C., Brinkac L.M., Lewis M., Johri S., Weaver B., Pai G., Eisen J.A., Rahe E., Sheldon W.M. , Ye W., Miller T.R., Carlton J., Rasko D.A., Paulsen I.T., Ren Q., Daugherty S.C., DeBoy R.T., Dodson R.J., Durkin A.S., Madupu R., Nelson W.C., Sullivan S.A., Rosovitz M.J., Haft D.H., Selengut J., Ward N. Nature 432:910-913(2004) [PubMed: 15602564] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700808 / DSM 15171 / DSS-3.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000031 Genomic DNA. Translation: AAV93978.1.
RefSeq	YP_165925.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3195523.
GenomeReviews	Gene locus SPO0670 in contig CP000031_GR.
KEGG	sil:SPO0670.
NMPDR	fig|246200.3.peg.576.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5LVN0.
Enzyme and pathway databases
BioCyc	SPOM246200:SPO_0670-MON.
Family and domain databases
HAMAP	MF_01902. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. PHP_N. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_SILPO

Accession

Primary (citable) accession number: Q5LVN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	February 1, 2005
Last modified:	November 25, 2008
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)