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Protein

Ectoine/5-hydroxyectoine-binding periplasmic protein UehA

Gene

uehA

Organism
Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter pomeroyi)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the tripartite ATP-independent periplasmic (TRAP) transport system UehABC, which imports both ectoine and 5-hydroxyectoine as nutrients, and not as osmoprotectants. UehA binds both ectoine and 5-hydroxyectoine with high specificity and affinity.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361Ectoine1 Publication
Binding sitei171 – 1711Ectoine1 Publication
Binding sitei211 – 2111Ectoine1 Publication
Binding sitei215 – 2151Ectoine1 Publication
Binding sitei236 – 2361Ectoine1 Publication

GO - Molecular functioni

  • C4-dicarboxylate transmembrane transporter activity Source: TIGR

GO - Biological processi

  • C4-dicarboxylate transport Source: TIGR
Complete GO annotation...

Keywords - Biological processi

Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Ectoine/5-hydroxyectoine-binding periplasmic protein UehACurated
Alternative name(s):
Uptake of ectoine and hydroxyectoine protein ACurated
Gene namesi
Name:uehA1 Publication
Ordered Locus Names:SPO1147Imported
OrganismiRuegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter pomeroyi)
Taxonomic identifieri246200 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRuegeria
Proteomesi
  • UP000001023 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 339319Ectoine/5-hydroxyectoine-binding periplasmic protein UehAPRO_5004259469Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi162 ↔ 3031 Publication

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Monomer (PubMed:19362561). The complex comprises the extracytoplasmic solute receptor protein UehA, and the two transmembrane proteins UehB and UehC (Probable).1 Publication1 Publication

Protein-protein interaction databases

STRINGi246200.SPO1147.

Structurei

Secondary structure

1
339
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi31 – 333Combined sources
Helixi41 – 5515Combined sources
Beta strandi56 – 583Combined sources
Beta strandi62 – 643Combined sources
Helixi72 – 809Combined sources
Beta strandi85 – 884Combined sources
Helixi91 – 944Combined sources
Turni95 – 973Combined sources
Helixi99 – 1057Combined sources
Helixi114 – 12310Combined sources
Helixi125 – 1284Combined sources
Helixi132 – 1365Combined sources
Turni137 – 1393Combined sources
Beta strandi140 – 15718Combined sources
Helixi162 – 1643Combined sources
Beta strandi169 – 1724Combined sources
Helixi176 – 18510Combined sources
Beta strandi187 – 1915Combined sources
Helixi194 – 1963Combined sources
Helixi197 – 2026Combined sources
Beta strandi208 – 2125Combined sources
Helixi213 – 2186Combined sources
Helixi221 – 2233Combined sources
Beta strandi226 – 2305Combined sources
Beta strandi235 – 2439Combined sources
Helixi244 – 2496Combined sources
Helixi252 – 27726Combined sources
Helixi280 – 2878Combined sources
Beta strandi292 – 2954Combined sources
Helixi298 – 33538Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3FXBX-ray2.90A/B28-339[»]
ProteinModelPortaliQ5LUA7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5LUA7.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108KJ1. Bacteria.
COG1638. LUCA.
HOGENOMiHOG000224244.
OMAiGESADIM.
OrthoDBiPOG091H03NF.

Family and domain databases

InterProiIPR018389. TRAP_DctP/TeaA.
[Graphical view]
PfamiPF03480. DctP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5LUA7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQSITFTFG AVAAAGIALA AGTAAQADTW RYAFEEAMTD VQGVYAQKFK
60 70 80 90 100
EEIEANSDHE IQLFPYGTLG ESADIMEQTQ DGILQFVDQS PGFTGSLIPE
110 120 130 140 150
AQVFFVPYLL PTDQDHLARF FKESKAINDM FKPLYADQGL ELLNMFPEGE
160 170 180 190 200
VAMTTKTPVT TCSDLDEVKF RVMTNPLLVE SYKAFGATPT PLPWGEVYGG
210 220 230 240 250
LQTNVIQGQE NPTFFLYSTK IYEVTDYITY AGHNNFTTAV MANKDFYDGL
260 270 280 290 300
SAEDQQLVQN AALAAYDHTV VYQQQAADTE LAKIMEAKPE MQVTVLTDEQ
310 320 330
RSCFKEAAAE VEAKFIEMTG DSGAAILKQM KADLAATAN
Length:339
Mass (Da):37,276
Last modified:February 1, 2005 - v1
Checksum:i174B38DFCC11354E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000031 Genomic DNA. Translation: AAV94447.1.
RefSeqiWP_011046894.1. NC_003911.12.

Genome annotation databases

EnsemblBacteriaiAAV94447; AAV94447; SPO1147.
KEGGisil:SPO1147.
PATRICi23375597. VBIRuePom114501_1169.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000031 Genomic DNA. Translation: AAV94447.1.
RefSeqiWP_011046894.1. NC_003911.12.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3FXBX-ray2.90A/B28-339[»]
ProteinModelPortaliQ5LUA7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246200.SPO1147.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAV94447; AAV94447; SPO1147.
KEGGisil:SPO1147.
PATRICi23375597. VBIRuePom114501_1169.

Phylogenomic databases

eggNOGiENOG4108KJ1. Bacteria.
COG1638. LUCA.
HOGENOMiHOG000224244.
OMAiGESADIM.
OrthoDBiPOG091H03NF.

Miscellaneous databases

EvolutionaryTraceiQ5LUA7.

Family and domain databases

InterProiIPR018389. TRAP_DctP/TeaA.
[Graphical view]
PfamiPF03480. DctP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUEHA_RUEPO
AccessioniPrimary (citable) accession number: Q5LUA7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2016
Last sequence update: February 1, 2005
Last modified: September 7, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Transport is enhanced in cells that are grown in the presence of ectoine, but not by high-salinity media.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.