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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter pomeroyi)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 146FADUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Synonyms:gid
Ordered Locus Names:SPO1686
OrganismiRuegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter pomeroyi)
Taxonomic identifieri246200 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRuegeria
Proteomesi
  • UP000001023 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 449449Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOPRO_0000117258Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi246200.SPO1686.

Structurei

3D structure databases

ProteinModelPortaliQ5LST0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiPOG091H00B3.

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5LST0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDRLHIVGG GMAGSEAAWQ AAQQGIDVVI HEMRPTVGTF AHQTGNLAEM
60 70 80 90 100
VCSNSFRSDD DEQNAVGLLH WEMRAAGGLI MSAADAHRLP AGGALAVDRD
110 120 130 140 150
PFAETVTARL HAHPRVSVTA EEVTELPRDG HWIFATGPLT SPALGAAIQA
160 170 180 190 200
ETGAEALAFF DAIAPIVYAD TIDMSRAWMQ SRYDKGETEE ERTAYLNCPM
210 220 230 240 250
DRDQYEAFID ALLAADKTEF HEGETATYFD GCLPIEVMAE RGRETLRHGP
260 270 280 290 300
MKPVGLTNPH QPDVKAYAVV QLRRDNALGT LYNIVGFQTK MKYGAQTAVF
310 320 330 340 350
KTIPGLENAS FARLGGIHRN TFLNSPTLLD AQMRMKSRPN IRFAGQITGV
360 370 380 390 400
EGYVESAAMG LLAGRLAAAE IQGRALPEVP QDSAMGALIH HITGGAEAKT
410 420 430 440
FQPMNVNFGL FRPVDGLKGG RRGRKDRYKA YTDRAKEAWT SWLAEAENG
Length:449
Mass (Da):48,813
Last modified:February 1, 2005 - v1
Checksum:iF12AA72DC910C953
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000031 Genomic DNA. Translation: AAV94971.1.
RefSeqiWP_011047421.1. NC_003911.12.

Genome annotation databases

EnsemblBacteriaiAAV94971; AAV94971; SPO1686.
KEGGisil:SPO1686.
PATRICi23376697. VBIRuePom114501_1713.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000031 Genomic DNA. Translation: AAV94971.1.
RefSeqiWP_011047421.1. NC_003911.12.

3D structure databases

ProteinModelPortaliQ5LST0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246200.SPO1686.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAV94971; AAV94971; SPO1686.
KEGGisil:SPO1686.
PATRICi23376697. VBIRuePom114501_1713.

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiPOG091H00B3.

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRMFO_RUEPO
AccessioniPrimary (citable) accession number: Q5LST0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: February 1, 2005
Last modified: September 7, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.