Reviewed, UniProtKB/Swiss-Prot Q5LSJ4 (3HAO_SILPO)

Names and origin

Protein names

Recommended name:
    3-hydroxyanthranilate 3,4-dioxygenase
    EC=1.13.11.6
Alternative name(s):
    3-hydroxyanthranilic acid dioxygenase
      Short name=HAD
    3-hydroxyanthranilate oxygenase
      Short name=3-HAO

Gene names

Ordered Locus Names:

SPO1774

Organism

Silicibacter pomeroyi [Complete proteome] [HAMAP]

Taxonomic identifier

89184 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Ruegeria

Protein attributes

Sequence length	180 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidative ring opening of 3-hydroxyanthranilate to 2-amino-3-carboxymuconate semialdehyde, which spontaneously cyclizes to quinolinate By similarity. HAMAP MF_00825
Catalytic activity	3-hydroxyanthranilate + O2 = 2-amino-3-carboxymuconate semialdehyde. HAMAP MF_00825
Cofactor	Binds 2 Fe2+ ions per subunit By similarity. HAMAP MF_00825
Subunit structure	Homodimer By similarity. HAMAP MF_00825
Sequence similarities	Belongs to the 3-HAO family.

Ontologies

Keywords
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-hydroxyanthranilate 3,4-dioxygenase activity Inferred from electronic annotation. Source: HAMAP ferrous iron binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 180	180	3-hydroxyanthranilate 3,4-dioxygenase HAMAP MF_00825		PRO_0000245478
Sites
	Metal binding	50	1	Iron 1; catalytic By similarity
	Metal binding	56	1	Iron 1; catalytic By similarity
	Metal binding	94	1	Iron 1; catalytic By similarity
	Metal binding	124	1	Iron 2 By similarity
	Metal binding	127	1	Iron 2 By similarity
	Metal binding	161	1	Iron 2 By similarity
	Metal binding	164	1	Iron 2 By similarity
	Binding site	46	1	Dioxygen By similarity
	Binding site	56	1	Substrate By similarity
	Binding site	98	1	Substrate By similarity
	Binding site	109	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5LSJ4-1 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 53B08A94EFFE38BA	FASTA	180	20,575
	10 20 30 40 50 60 MARLSAFNFQ KWIDEHKHLL KPPVGNQQVW EDADLMVTVV GGPNKRTDYH DDPVEEFFYQ 70 80 90 100 110 120 LKGDMVLKLY EGGEFYDVPI REGDIFLLPP HVRHSPQRPQ EGSIGLVIEP KRPEGAHDAI 130 140 150 160 170 180 EWFCFGCGSL VHRAELLLES IVRDLPPVYQ AFYADEQART CPNCGEIHPG KEPPEGWVKL

References

[1]

"Genome sequence of Silicibacter pomeroyi reveals adaptations to the marine environment." Moran M.A., Buchan A., Gonzalez J.M., Heidelberg J.F., Whitman W.B., Kiene R.P., Henriksen J.R., King G.M., Belas R., Fuqua C., Brinkac L.M., Lewis M., Johri S., Weaver B., Pai G., Eisen J.A., Rahe E., Sheldon W.M. , Ye W., Miller T.R., Carlton J., Rasko D.A., Paulsen I.T., Ren Q., Daugherty S.C., DeBoy R.T., Dodson R.J., Durkin A.S., Madupu R., Nelson W.C., Sullivan S.A., Rosovitz M.J., Haft D.H., Selengut J., Ward N. Nature 432:910-913(2004) [PubMed: 15602564] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700808 / DSM 15171 / DSS-3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000031 Genomic DNA. Translation: AAV95053.1.
RefSeq	YP_167011.1.
3D structure databases
SMR	Q5LSJ4. Positions 5-171.
ModBase	Search...
Genome annotation databases
GeneID	3192877.
GenomeReviews	Gene locus SPO1774 in contig CP000031_GR.
KEGG	sil:SPO1774.
NMPDR	fig|246200.3.peg.2180.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG341987.
OMA	RHSPQRP.
Enzyme and pathway databases
BioCyc	RPOM246200:SPO_1774-MONOMER.
BRENDA	1.13.11.6. 278247.
Family and domain databases
HAMAP	MF_00825. 3_HAO. [Tree]
InterPro	IPR010329. 3hydroanth_dOase. IPR011051. Cupin_RmlC_type. [Graphical view]
Pfam	PF06052. 3-HAO. 1 hit. [Graphical view]
TIGRFAMs	TIGR03037. anthran_nbaC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

3HAO_SILPO

Accession

Primary (citable) accession number: Q5LSJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
Last sequence update:	February 1, 2005
Last modified:	February 9, 2010
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)