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Protein

Queuine tRNA-ribosyltransferase

Gene

tgt

Organism
Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter pomeroyi)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).UniRule annotation

Catalytic activityi

Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine.UniRule annotation
Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei93 – 931NucleophileUniRule annotation
Binding sitei94 – 941SubstrateUniRule annotation
Metal bindingi305 – 3051ZincUniRule annotation
Metal bindingi307 – 3071ZincUniRule annotation
Metal bindingi310 – 3101ZincUniRule annotation
Metal bindingi336 – 3361ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Queuosine biosynthesis, tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
Queuine tRNA-ribosyltransferaseUniRule annotation (EC:2.4.2.29UniRule annotation)
Alternative name(s):
Guanine insertion enzymeUniRule annotation
tRNA-guanine transglycosylaseUniRule annotation
Gene namesi
Name:tgtUniRule annotation
Ordered Locus Names:SPO2616
OrganismiRuegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter pomeroyi)
Taxonomic identifieri246200 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRuegeria
Proteomesi
  • UP000001023 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 376376Queuine tRNA-ribosyltransferasePRO_0000135521Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi246200.SPO2616.

Structurei

3D structure databases

ProteinModelPortaliQ5LQ80.
SMRiQ5LQ80. Positions 4-366.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the queuine tRNA-ribosyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C6U. Bacteria.
COG0343. LUCA.
HOGENOMiHOG000223473.
KOiK00773.
OMAiMGVGKPD.
OrthoDBiEOG6SNDVG.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5LQ80-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSERFSFDLH ASDGKARTGV IHTPRGGVRT PAFMPVGTAA TVKAMMPESV
60 70 80 90 100
RATGADILLG NTYHLMLRPT AERIDRLGGL HRFMNWDRPI LTDSGGFQVM
110 120 130 140 150
SLAGLRKLTE KGVTFKSHID GSKHELTPER SMEIQRLLGS DIVMCFDECP
160 170 180 190 200
ALPADRDRIA ESMRLSMRWA ERSREAFGDR PGHALFGIMQ GGLEQDLREE
210 220 230 240 250
SAQALRSVGF DGYAVGGLAV GEGQAAMFDC LDYAPGFLPE DKPRYLMGVG
260 270 280 290 300
KPDDIVGAVK RGIDMMDCVL PSRSGRTGQV FTRHGVLNIK NARHMDDPRP
310 320 330 340 350
LDEACTCPAC RNYSRAYLHH VFRSQEIISS MLLTWHNLHY FQEIMQGMRE
360 370
AIAGGTFEAW EAAFHAGRAQ GDIEPL
Length:376
Mass (Da):41,641
Last modified:February 1, 2005 - v1
Checksum:i78BCEE70A843D24D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000031 Genomic DNA. Translation: AAV95861.1.
RefSeqiWP_011048318.1. NC_003911.12.

Genome annotation databases

EnsemblBacteriaiAAV95861; AAV95861; SPO2616.
KEGGisil:SPO2616.
PATRICi23378639. VBIRuePom114501_2666.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000031 Genomic DNA. Translation: AAV95861.1.
RefSeqiWP_011048318.1. NC_003911.12.

3D structure databases

ProteinModelPortaliQ5LQ80.
SMRiQ5LQ80. Positions 4-366.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246200.SPO2616.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAV95861; AAV95861; SPO2616.
KEGGisil:SPO2616.
PATRICi23378639. VBIRuePom114501_2666.

Phylogenomic databases

eggNOGiENOG4105C6U. Bacteria.
COG0343. LUCA.
HOGENOMiHOG000223473.
KOiK00773.
OMAiMGVGKPD.
OrthoDBiEOG6SNDVG.

Enzyme and pathway databases

UniPathwayiUPA00392.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700808 / DSM 15171 / DSS-3.
  2. "An updated genome annotation for the model marine bacterium Ruegeria pomeroyi DSS-3."
    Rivers A.R., Smith C.B., Moran M.A.
    Stand. Genomic Sci. 9:11-11(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: ATCC 700808 / DSM 15171 / DSS-3.

Entry informationi

Entry nameiTGT_RUEPO
AccessioniPrimary (citable) accession number: Q5LQ80
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: February 1, 2005
Last modified: November 11, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.