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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter pomeroyi)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei608Aminoacyl-adenylateUniRule annotation1
Binding sitei652ATPUniRule annotation1
Metal bindingi943ZincUniRule annotation1
Metal bindingi946ZincUniRule annotation1
Metal bindingi962ZincUniRule annotation1
Metal bindingi965ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciRPOM246200:G1G48-3186-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:SPO3136
OrganismiRuegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter pomeroyi)
Taxonomic identifieri246200 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRuegeria
Proteomesi
  • UP000001023 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000984621 – 970Isoleucine--tRNA ligaseAdd BLAST970

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi246200.SPO3136

Structurei

3D structure databases

ProteinModelPortaliQ5LNR7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi65 – 75"HIGH" regionAdd BLAST11
Motifi649 – 653"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q5LNR7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCADTTAETP EYKDTLNLPK TDFPMRAGLP AREPQWLERW EKIGVYDRLR
60 70 80 90 100
EKQGRAPFTL HDGPPYANGH LHIGHALNKT IKDMIVRSHQ MMGFDARYVP
110 120 130 140 150
GWDCHGLPIE WKIEEQYRKK GKSKDDVNVV EFRQECRRFA EGWIDIQREE
160 170 180 190 200
FKRLGITGNW ADPYVTMDYH AEAVIADEFM KFLMNGTLYQ GSKPVMWSPI
210 220 230 240 250
EQTALAEAEV EYHDKESFTI WVKFRAVNSG DLDGAQVVIW TTTPWTIPSN
260 270 280 290 300
KAVVYGKDIA YGLYEITGTP EECWASVGDK YILADKLADD VMRRARLDPD
310 320 330 340 350
MYRRVGDVDP SQIGGLTHPL HGAEGGNGEW DDIRDFRAAE FVTDTEGTGF
360 370 380 390 400
VHCAPSHGME EFELYRDLGM LEQVITYNVM DDGSFRADLP FFGGKYILSR
410 420 430 440 450
KGGEGDANKT VIDKLVEVGG LLARGKIKHS YPHSWRSKAP IIYRNTPQWF
460 470 480 490 500
ASVDRPVNDG QDSYGKTIRE RALTSIDQLV KFTPQTGRNR LYSMIEARPD
510 520 530 540 550
WVLSRQRAWG VPLTCFTRKG ALPTDADFLL RDPAVNARIF AAFEAEGADC
560 570 580 590 600
WYAEGAKERF LGNDYKADEW DQVFDVLDVW FDSGSTHAFV LRDREDGTAD
610 620 630 640 650
GIADVYMEGT DQHRGWFHSS LLQACGTLGR APYRNVVTHG FTLDEKGMKM
660 670 680 690 700
SKSLGNTIVP DEVVKQYGAD ILRLWVAQTD YTADQRIGPE ILKGVADSYR
710 720 730 740 750
RLRNTMRFML GSLSDFTEAD RIEDPAEMPP LERWVLSRMA ELDEQVRKGY
760 770 780 790 800
AGFDFQGVYS AVFNFATVDL SAFYFDIRKD VLYCDGDTTR RRAARTVLDL
810 820 830 840 850
LFHRLTTWLA PILVFTMEEV WLERYPGEGS SVHLVDFPET PASWRNPQNE
860 870 880 890 900
SNVARVRRVR RVVTAALEIQ RRDKVIGSSL EAAPVVHVED AETRALLAQI
910 920 930 940 950
DIDDLCITSG LTVSADPAPA EAFRLPEIEG VGVVFEMAEG EKCQRCWKIL
960 970
PDVGRHAHAG VCGRCDAALG
Length:970
Mass (Da):109,796
Last modified:February 1, 2005 - v1
Checksum:i6A84B8DB96390B75
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000031 Genomic DNA Translation: AAV96371.1
RefSeqiWP_011048826.1, NC_003911.12

Genome annotation databases

EnsemblBacteriaiAAV96371; AAV96371; SPO3136
KEGGisil:SPO3136

Similar proteinsi

Entry informationi

Entry nameiSYI_RUEPO
AccessioniPrimary (citable) accession number: Q5LNR7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: February 1, 2005
Last modified: March 28, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health