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Q5LNR7

- SYI_RUEPO

UniProt

Q5LNR7 - SYI_RUEPO

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Protein
Isoleucine--tRNA ligase
Gene
ileS, SPO3136
Organism
Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter pomeroyi)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei608 – 6081Aminoacyl-adenylate By similarity
Binding sitei652 – 6521ATP By similarity
Metal bindingi943 – 9431Zinc By similarity
Metal bindingi946 – 9461Zinc By similarity
Metal bindingi962 – 9621Zinc By similarity
Metal bindingi965 – 9651Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:SPO3136
OrganismiRuegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter pomeroyi)
Taxonomic identifieri246200 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRuegeria
ProteomesiUP000001023: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 970970Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098462Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi246200.SPO3136.

Structurei

3D structure databases

ProteinModelPortaliQ5LNR7.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi65 – 7511"HIGH" regionUniRule annotation
Add
BLAST
Motifi649 – 6535"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiERLMLHQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5LNR7-1 [UniParc]FASTAAdd to Basket

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MCADTTAETP EYKDTLNLPK TDFPMRAGLP AREPQWLERW EKIGVYDRLR    50
EKQGRAPFTL HDGPPYANGH LHIGHALNKT IKDMIVRSHQ MMGFDARYVP 100
GWDCHGLPIE WKIEEQYRKK GKSKDDVNVV EFRQECRRFA EGWIDIQREE 150
FKRLGITGNW ADPYVTMDYH AEAVIADEFM KFLMNGTLYQ GSKPVMWSPI 200
EQTALAEAEV EYHDKESFTI WVKFRAVNSG DLDGAQVVIW TTTPWTIPSN 250
KAVVYGKDIA YGLYEITGTP EECWASVGDK YILADKLADD VMRRARLDPD 300
MYRRVGDVDP SQIGGLTHPL HGAEGGNGEW DDIRDFRAAE FVTDTEGTGF 350
VHCAPSHGME EFELYRDLGM LEQVITYNVM DDGSFRADLP FFGGKYILSR 400
KGGEGDANKT VIDKLVEVGG LLARGKIKHS YPHSWRSKAP IIYRNTPQWF 450
ASVDRPVNDG QDSYGKTIRE RALTSIDQLV KFTPQTGRNR LYSMIEARPD 500
WVLSRQRAWG VPLTCFTRKG ALPTDADFLL RDPAVNARIF AAFEAEGADC 550
WYAEGAKERF LGNDYKADEW DQVFDVLDVW FDSGSTHAFV LRDREDGTAD 600
GIADVYMEGT DQHRGWFHSS LLQACGTLGR APYRNVVTHG FTLDEKGMKM 650
SKSLGNTIVP DEVVKQYGAD ILRLWVAQTD YTADQRIGPE ILKGVADSYR 700
RLRNTMRFML GSLSDFTEAD RIEDPAEMPP LERWVLSRMA ELDEQVRKGY 750
AGFDFQGVYS AVFNFATVDL SAFYFDIRKD VLYCDGDTTR RRAARTVLDL 800
LFHRLTTWLA PILVFTMEEV WLERYPGEGS SVHLVDFPET PASWRNPQNE 850
SNVARVRRVR RVVTAALEIQ RRDKVIGSSL EAAPVVHVED AETRALLAQI 900
DIDDLCITSG LTVSADPAPA EAFRLPEIEG VGVVFEMAEG EKCQRCWKIL 950
PDVGRHAHAG VCGRCDAALG 970
Length:970
Mass (Da):109,796
Last modified:February 1, 2005 - v1
Checksum:i6A84B8DB96390B75
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000031 Genomic DNA. Translation: AAV96371.1.
RefSeqiYP_168339.1. NC_003911.12.

Genome annotation databases

EnsemblBacteriaiAAV96371; AAV96371; SPO3136.
GeneIDi3193197.
KEGGisil:SPO3136.
PATRICi23379717. VBIRuePom114501_3202.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000031 Genomic DNA. Translation: AAV96371.1 .
RefSeqi YP_168339.1. NC_003911.12.

3D structure databases

ProteinModelPortali Q5LNR7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 246200.SPO3136.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAV96371 ; AAV96371 ; SPO3136 .
GeneIDi 3193197.
KEGGi sil:SPO3136.
PATRICi 23379717. VBIRuePom114501_3202.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi ERLMLHQ.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700808 / DSM 15171 / DSS-3.

Entry informationi

Entry nameiSYI_RUEPO
AccessioniPrimary (citable) accession number: Q5LNR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: February 1, 2005
Last modified: May 14, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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