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Q5LNR7

- SYI_RUEPO

UniProt

Q5LNR7 - SYI_RUEPO

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter pomeroyi)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei608 – 6081Aminoacyl-adenylateUniRule annotation
Binding sitei652 – 6521ATPUniRule annotation
Metal bindingi943 – 9431ZincUniRule annotation
Metal bindingi946 – 9461ZincUniRule annotation
Metal bindingi962 – 9621ZincUniRule annotation
Metal bindingi965 – 9651ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:SPO3136
OrganismiRuegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter pomeroyi)
Taxonomic identifieri246200 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRuegeria
ProteomesiUP000001023: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 970970Isoleucine--tRNA ligasePRO_0000098462Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi246200.SPO3136.

Structurei

3D structure databases

ProteinModelPortaliQ5LNR7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi65 – 7511"HIGH" regionAdd
BLAST
Motifi649 – 6535"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiERLMLHQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5LNR7 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCADTTAETP EYKDTLNLPK TDFPMRAGLP AREPQWLERW EKIGVYDRLR
60 70 80 90 100
EKQGRAPFTL HDGPPYANGH LHIGHALNKT IKDMIVRSHQ MMGFDARYVP
110 120 130 140 150
GWDCHGLPIE WKIEEQYRKK GKSKDDVNVV EFRQECRRFA EGWIDIQREE
160 170 180 190 200
FKRLGITGNW ADPYVTMDYH AEAVIADEFM KFLMNGTLYQ GSKPVMWSPI
210 220 230 240 250
EQTALAEAEV EYHDKESFTI WVKFRAVNSG DLDGAQVVIW TTTPWTIPSN
260 270 280 290 300
KAVVYGKDIA YGLYEITGTP EECWASVGDK YILADKLADD VMRRARLDPD
310 320 330 340 350
MYRRVGDVDP SQIGGLTHPL HGAEGGNGEW DDIRDFRAAE FVTDTEGTGF
360 370 380 390 400
VHCAPSHGME EFELYRDLGM LEQVITYNVM DDGSFRADLP FFGGKYILSR
410 420 430 440 450
KGGEGDANKT VIDKLVEVGG LLARGKIKHS YPHSWRSKAP IIYRNTPQWF
460 470 480 490 500
ASVDRPVNDG QDSYGKTIRE RALTSIDQLV KFTPQTGRNR LYSMIEARPD
510 520 530 540 550
WVLSRQRAWG VPLTCFTRKG ALPTDADFLL RDPAVNARIF AAFEAEGADC
560 570 580 590 600
WYAEGAKERF LGNDYKADEW DQVFDVLDVW FDSGSTHAFV LRDREDGTAD
610 620 630 640 650
GIADVYMEGT DQHRGWFHSS LLQACGTLGR APYRNVVTHG FTLDEKGMKM
660 670 680 690 700
SKSLGNTIVP DEVVKQYGAD ILRLWVAQTD YTADQRIGPE ILKGVADSYR
710 720 730 740 750
RLRNTMRFML GSLSDFTEAD RIEDPAEMPP LERWVLSRMA ELDEQVRKGY
760 770 780 790 800
AGFDFQGVYS AVFNFATVDL SAFYFDIRKD VLYCDGDTTR RRAARTVLDL
810 820 830 840 850
LFHRLTTWLA PILVFTMEEV WLERYPGEGS SVHLVDFPET PASWRNPQNE
860 870 880 890 900
SNVARVRRVR RVVTAALEIQ RRDKVIGSSL EAAPVVHVED AETRALLAQI
910 920 930 940 950
DIDDLCITSG LTVSADPAPA EAFRLPEIEG VGVVFEMAEG EKCQRCWKIL
960 970
PDVGRHAHAG VCGRCDAALG
Length:970
Mass (Da):109,796
Last modified:February 1, 2005 - v1
Checksum:i6A84B8DB96390B75
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000031 Genomic DNA. Translation: AAV96371.1.
RefSeqiYP_168339.1. NC_003911.12.

Genome annotation databases

EnsemblBacteriaiAAV96371; AAV96371; SPO3136.
GeneIDi3193197.
KEGGisil:SPO3136.
PATRICi23379717. VBIRuePom114501_3202.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000031 Genomic DNA. Translation: AAV96371.1 .
RefSeqi YP_168339.1. NC_003911.12.

3D structure databases

ProteinModelPortali Q5LNR7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 246200.SPO3136.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAV96371 ; AAV96371 ; SPO3136 .
GeneIDi 3193197.
KEGGi sil:SPO3136.
PATRICi 23379717. VBIRuePom114501_3202.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi ERLMLHQ.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700808 / DSM 15171 / DSS-3.

Entry informationi

Entry nameiSYI_RUEPO
AccessioniPrimary (citable) accession number: Q5LNR7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: February 1, 2005
Last modified: October 29, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3