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Q5LMY0 (SYL_RUEPO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:SPO3432
OrganismRuegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter pomeroyi) [Complete proteome] [HAMAP]
Taxonomic identifier246200 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRuegeria

Protein attributes

Sequence length852 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 852852Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152081

Regions

Motif41 – 5111"HIGH" region HAMAP-Rule MF_00049
Motif623 – 6275"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6261ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5LMY0 [UniParc].

Last modified February 1, 2005. Version 1.
Checksum: 52EB8247D0D84839

FASTA85294,818
        10         20         30         40         50         60 
MSRYSATEIE AKWQEAWDKA GIFRAARTAD KPKYYVLEMF PYPSGRIHIG HVRNYTMGDV 

        70         80         90        100        110        120 
IARYKLSNGF NVLHPMGFDA FGMPAENAAM ASGGHPKDWT YKNIETMVAQ MKPLGFGLDW 

       130        140        150        160        170        180 
SRMFATCDPE YYGQQQALFL DFLEKGLVYR KNAVVNWDPV DMTVLANEQV EDGRGWRSGA 

       190        200        210        220        230        240 
LVERRELTQW FFKISDMSGE LLDALDTLDD WPAKVKLMQA NWIGKSRGLQ FSWALTEPAH 

       250        260        270        280        290        300 
GIEALDVYTT RPDTLMGASF LGISPDHPLT KALEAENPDL AAKVAEMRKG GTTEEAIEKA 

       310        320        330        340        350        360 
EKLGADTGLR VRHPLNPDWE LPVWVANFIL MDYGTGAIFA CPAHDQRDLD FCRKYDLPVI 

       370        380        390        400        410        420 
DTFVALDDPK PIATEAFVPL KTEKVKWIDH FAGLDVATGQ EAIDTTIDWM EAKGLGTGVT 

       430        440        450        460        470        480 
KFRLRDWGLS RQRYWGCPIP VVHCDACGVV PEKKENLPIR LPDDVTFDVP GNPLDRHPTW 

       490        500        510        520        530        540 
RDCACPSCGK PARRETDTMD TFVDSSWYFA RFTAPRAETP TDMAEAEYWM NVDQYIGGIE 

       550        560        570        580        590        600 
HAILHLLYSR FFARAMHICG HLPEKSKEPF NALFTQGMVT HAIYKTTGAD DRPVYHYPEA 

       610        620        630        640        650        660 
VRLEGGKGYL EDGTEVEIIP SAKMSKSKNN VVDPVAIIDR FGADTARWFV LSDSPPERDV 

       670        680        690        700        710        720 
EWTDAGAEAA HKHLNRVWNL CDRIAAMDSA APGEGDDDLL REMHKCIRDV TNGIESFGFN 

       730        740        750        760        770        780 
ASIAKLYAFT NTLAKSKAGA AAQKQAIMTL AQLMSPMTPH LAEDIWAHQG GEGLIAAAPW 

       790        800        810        820        830        840 
PVADEAMLVD ALVTLPVQIN GKRRAEISVP ADMDKTQVEK LALSTDAVQK ALDGAAPKKV 

       850 
IVVPGRIVNV VV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000031 Genomic DNA. Translation: AAV96658.1.
RefSeqYP_168628.1. NC_003911.12.

3D structure databases

ProteinModelPortalQ5LMY0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING246200.SPO3432.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAV96658; AAV96658; SPO3432.
GeneID3192670.
KEGGsil:SPO3432.
PATRIC23380325. VBIRuePom114501_3499.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OrthoDBEOG63Z74X.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_RUEPO
AccessionPrimary (citable) accession number: Q5LMY0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: February 1, 2005
Last modified: May 14, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries