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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter pomeroyi)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei626 – 6261ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:SPO3432
OrganismiRuegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter pomeroyi)
Taxonomic identifieri246200 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRuegeria
ProteomesiUP000001023 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 852852Leucine--tRNA ligasePRO_0000152081Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi246200.SPO3432.

Structurei

3D structure databases

ProteinModelPortaliQ5LMY0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi41 – 5111"HIGH" regionAdd
BLAST
Motifi623 – 6275"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiLHMGHAE.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5LMY0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRYSATEIE AKWQEAWDKA GIFRAARTAD KPKYYVLEMF PYPSGRIHIG
60 70 80 90 100
HVRNYTMGDV IARYKLSNGF NVLHPMGFDA FGMPAENAAM ASGGHPKDWT
110 120 130 140 150
YKNIETMVAQ MKPLGFGLDW SRMFATCDPE YYGQQQALFL DFLEKGLVYR
160 170 180 190 200
KNAVVNWDPV DMTVLANEQV EDGRGWRSGA LVERRELTQW FFKISDMSGE
210 220 230 240 250
LLDALDTLDD WPAKVKLMQA NWIGKSRGLQ FSWALTEPAH GIEALDVYTT
260 270 280 290 300
RPDTLMGASF LGISPDHPLT KALEAENPDL AAKVAEMRKG GTTEEAIEKA
310 320 330 340 350
EKLGADTGLR VRHPLNPDWE LPVWVANFIL MDYGTGAIFA CPAHDQRDLD
360 370 380 390 400
FCRKYDLPVI DTFVALDDPK PIATEAFVPL KTEKVKWIDH FAGLDVATGQ
410 420 430 440 450
EAIDTTIDWM EAKGLGTGVT KFRLRDWGLS RQRYWGCPIP VVHCDACGVV
460 470 480 490 500
PEKKENLPIR LPDDVTFDVP GNPLDRHPTW RDCACPSCGK PARRETDTMD
510 520 530 540 550
TFVDSSWYFA RFTAPRAETP TDMAEAEYWM NVDQYIGGIE HAILHLLYSR
560 570 580 590 600
FFARAMHICG HLPEKSKEPF NALFTQGMVT HAIYKTTGAD DRPVYHYPEA
610 620 630 640 650
VRLEGGKGYL EDGTEVEIIP SAKMSKSKNN VVDPVAIIDR FGADTARWFV
660 670 680 690 700
LSDSPPERDV EWTDAGAEAA HKHLNRVWNL CDRIAAMDSA APGEGDDDLL
710 720 730 740 750
REMHKCIRDV TNGIESFGFN ASIAKLYAFT NTLAKSKAGA AAQKQAIMTL
760 770 780 790 800
AQLMSPMTPH LAEDIWAHQG GEGLIAAAPW PVADEAMLVD ALVTLPVQIN
810 820 830 840 850
GKRRAEISVP ADMDKTQVEK LALSTDAVQK ALDGAAPKKV IVVPGRIVNV

VV
Length:852
Mass (Da):94,818
Last modified:February 1, 2005 - v1
Checksum:i52EB8247D0D84839
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000031 Genomic DNA. Translation: AAV96658.1.
RefSeqiYP_168628.1. NC_003911.12.

Genome annotation databases

EnsemblBacteriaiAAV96658; AAV96658; SPO3432.
GeneIDi3192670.
KEGGisil:SPO3432.
PATRICi23380325. VBIRuePom114501_3499.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000031 Genomic DNA. Translation: AAV96658.1.
RefSeqiYP_168628.1. NC_003911.12.

3D structure databases

ProteinModelPortaliQ5LMY0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246200.SPO3432.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAV96658; AAV96658; SPO3432.
GeneIDi3192670.
KEGGisil:SPO3432.
PATRICi23380325. VBIRuePom114501_3499.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiLHMGHAE.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700808 / DSM 15171 / DSS-3.

Entry informationi

Entry nameiSYL_RUEPO
AccessioniPrimary (citable) accession number: Q5LMY0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: February 1, 2005
Last modified: April 1, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.