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Q5LLK8 (TRMB_RUEPO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:SPOA0014
Encoded onPlasmid megaplasmid Spo
OrganismRuegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter pomeroyi) [Complete proteome] [HAMAP]
Taxonomic identifier246200 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRuegeria

Protein attributes

Sequence length237 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Plasmid
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 237237tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000229198

Regions

Region214 – 2174Substrate binding By similarity

Sites

Active site1411 By similarity
Binding site671S-adenosyl-L-methionine By similarity
Binding site921S-adenosyl-L-methionine By similarity
Binding site1191S-adenosyl-L-methionine By similarity
Binding site1411S-adenosyl-L-methionine By similarity
Binding site1451Substrate By similarity
Binding site1771Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5LLK8 [UniParc].

Last modified February 1, 2005. Version 1.
Checksum: DD11D17FB6A8EC21

FASTA23727,037
        10         20         30         40         50         60 
MSKTTPDRPR RNFYGRIKGK TLKPNQRTYL DEDLTPLAPG PVSWQDNPTR QPLDLNALFG 

        70         80         90        100        110        120 
GRETWLEIGF GGGEHLVHQA ASNPGIGIIG AEPYINGVAM LLGKIRAAGG DNVRVHPGDV 

       130        140        150        160        170        180 
RDLFDVLPAQ SISRAFLLYP DPWPKKRHHR RRFVTPEHLE PLARVLKPGA IFRVATDIPD 

       190        200        210        220        230 
YVRQTLEEVP RAGFEWLAER PADWRQPWGD WISTRYEQKA LREGRVPHYL TFRRLEG 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000032 Genomic DNA. Translation: AAV97155.1.
RefSeqYP_164846.1. NC_006569.1.

3D structure databases

ProteinModelPortalQ5LLK8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING246200.SPOA0014.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAV97155; AAV97155; SPOA0014.
GeneID3196748.
KEGGsil:SPOA0014.
PATRIC23381328. VBIRuePom114501_3989.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073969.
KOK03439.
OMAIGCEPFV.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_RUEPO
AccessionPrimary (citable) accession number: Q5LLK8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: February 1, 2005
Last modified: April 16, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways