Reviewed, UniProtKB/Swiss-Prot Q5LIQ7 (AROK_BACFN)

Names and origin

Protein names

Recommended name:
    Shikimate kinase
      Short name=SK
    EC=2.7.1.71

Gene names

Name:	aroK
Ordered Locus Names:	BF0192

Organism

Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]

Taxonomic identifier

272559 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate By similarity. HAMAP MF_00109
Catalytic activity	ATP + shikimate = ADP + shikimate 3-phosphate. HAMAP MF_00109
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00109
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7. HAMAP MF_00109
Subunit structure	Monomer By similarity. HAMAP MF_00109
Subcellular location	Cytoplasm Probable HAMAP MF_00109.
Sequence similarities	Belongs to the shikimate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP shikimate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 179	179	Shikimate kinase HAMAP MF_00109		PRO_0000237843
Regions
	Nucleotide binding	11 – 16	6	ATP By similarity
Sites
	Metal binding	15	1	Magnesium By similarity
	Binding site	33	1	Substrate By similarity
	Binding site	57	1	Substrate By similarity
	Binding site	79	1	Substrate; via amide nitrogen By similarity
	Binding site	118	1	ATP By similarity
	Binding site	140	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5LIQ7-1 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: 92541C53EF864AA0	FASTA	179	20,697
	10 20 30 40 50 60 MIRIFLTGYM GAGKTTLGKA LARELHIPFI DLDWYIEERF HKTVGELFSE RGEASFRELE 70 80 90 100 110 120 KNMLHEVGEF EDVVISTGGG APCFFDNMEY MNRVGTTVFL DVDPKVLFSR LRVAKQQRPI 130 140 150 160 170 LQGKKDDELL DFIVQALEKR APFYRQANYI YCADKLEDRS QIETSVQQLR KLLNLHIAS

References

[1]

"Extensive DNA inversions in the B. fragilis genome control variable gene expression." Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V., Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A., Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A. , Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J., Barrell B.G., Parkhill J. Science 307:1463-1465(2005) [PubMed: 15746427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR626927 Genomic DNA. Translation: CAH05969.1.
RefSeq	YP_209931.1.
3D structure databases
SMR	Q5LIQ7. Positions 3-173.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5LIQ7.
Genome annotation databases
GeneID	3288273.
GenomeReviews	Gene locus BF0192 in contig CR626927_GR.
KEGG	bfs:BF0192.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0703.
HOGENOM	HBG335815.
OMA	MIISERV.
Enzyme and pathway databases
BioCyc	BFRA272559:BF0192-MONOMER.
Family and domain databases
HAMAP	MF_00109. Shikimate_kinase. [Tree]
InterPro	IPR000623. Shik_kinase. [Graphical view]
Pfam	PF01202. SKI. 1 hit. [Graphical view]
PRINTS	PR01100. SHIKIMTKNASE.
PROSITE	PS01128. SHIKIMATE_KINASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROK_BACFN

Accession

Primary (citable) accession number: Q5LIQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	June 21, 2005
Last modified:	January 19, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)