Reviewed, UniProtKB/Swiss-Prot Q5LIQ2 (ARGB_BACFN)

Names and origin

Protein names

Recommended name:
    Acetylglutamate kinase
    EC=2.7.2.8
Alternative name(s):
    NAG kinase
      Short name=AGK
    N-acetyl-L-glutamate 5-phosphotransferase

Gene names

Name:	argB
Ordered Locus Names:	BF0197

Organism

Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]

Taxonomic identifier

272559 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. HAMAP MF_00082
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4. HAMAP MF_00082
Subcellular location	Cytoplasm Probable HAMAP MF_00082.
Sequence similarities	Belongs to the acetylglutamate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP acetylglutamate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 261	261	Acetylglutamate kinase HAMAP MF_00082		PRO_0000264682
Regions
	Region	45 – 46	2	Substrate binding By similarity
Sites
	Binding site	67	1	Substrate By similarity
	Binding site	162	1	Substrate By similarity
	Site	13	1	Transition state stabilizer By similarity
	Site	228	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5LIQ2-1 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: DEEE56EAC5A7DB9D	FASTA	261	27,994
	10 20 30 40 50 60 MKEKMKEKLT VIKVGGKIVE EEATLNQLLN DFAAIEGHKV LVHGGGRSAT KIAAQLGIDS 70 80 90 100 110 120 KMVNGRRITD AETLKVVTMV YGGLVNKNIV AGLQARGVNA LGLTGADMNV IRSMKRPVKE 130 140 150 160 170 180 VDYGFVGDVE RVDSTLLSDL IHKGVVPVMA PLTHDGQGNM LNTNADTIAG ETAKALSAIF 190 200 210 220 230 240 DVTLVYCFEK KGVLRDENDD ESVIPQINHA EFQRYIAEGV IQGGMIPKLE NSFEAINAGV 250 260 SEVVITLASA IHTDGGTRIK K

References

[1]

"Extensive DNA inversions in the B. fragilis genome control variable gene expression." Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V., Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A., Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A. , Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J., Barrell B.G., Parkhill J. Science 307:1463-1465(2005) [PubMed: 15746427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR626927 Genomic DNA. Translation: CAH05974.1.
RefSeq	YP_209936.1.
3D structure databases
HSSP	HSSP built from PDB template 2BUF based on UniProtKB Q9HTN2.
SMR	Q5LIQ2. Positions 3-246.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5LIQ2.
Genome annotation databases
GeneID	3288278.
GenomeReviews	Gene locus BF0197 in contig CR626927_GR.
KEGG	bfs:BF0197.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0548.
HOGENOM	HBG497643.
OMA	MVLCGST.
Enzyme and pathway databases
BioCyc	BFRA272559:BF0197-MONOMER.
Family and domain databases
HAMAP	MF_00082_B. ArgB_B. [Tree]
InterPro	IPR004662. AcgluKinase. IPR001048. Asp/Glu/Uridylate_kinase. IPR011148. GlcNAc_kinase. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000728. NAGK. 1 hit.
TIGRFAMs	TIGR00761. argB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGB_BACFN

Accession

Primary (citable) accession number: Q5LIQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	June 21, 2005
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)