Q5LIN8 (FUCI_BACFN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-fucose isomerase
EC=5.3.1.25

Alternative name(s):
6-deoxy-L-galactose isomerase
FucIase

Gene names

Name:	fucI
Ordered Locus Names:	BF0211

Organism

Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]

Taxonomic identifier

272559 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Bacteroidetes/Chlorobi group › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides › Bacteroides fragilis

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts the aldose L-fucose into the corresponding ketose L-fuculose By similarity. HAMAP-Rule MF_01254
Catalytic activity	L-fucose = L-fuculose. HAMAP-Rule MF_01254
Cofactor	Manganese By similarity.
Pathway	Carbohydrate degradation; L-fucose degradation; L-lactaldehyde and glycerone phosphate from L-fucose: step 1/3. HAMAP-Rule MF_01254
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the L-fucose isomerase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Fucose metabolism
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	fucose catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	L-fucose isomerase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 590	590	L-fucose isomerase HAMAP-Rule MF_01254		PRO_1000067213
Sites
	Active site	337	1	Proton acceptor By similarity
	Active site	361	1	Proton acceptor By similarity
	Metal binding	337	1	Manganese By similarity
	Metal binding	361	1	Manganese By similarity
	Metal binding	528	1	Manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5LIN8 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: 058D6B26DFD8DD08	FASTA	590	65,371
	10 20 30 40 50 60 MKKYPKIGIR PTIDGRQGGV RESLEEKTMN LAKAVAELIT SNLKNGDGTP VECVIADGTI 70 80 90 100 110 120 GRVAESAACA EKFEREGVGA TITVTSCWCY GAETMDMNPY YPKAVWGFNG TERPGAVYLA 130 140 150 160 170 180 AVLAGHAQKG LPAFGIYGRD VQDLNDNSIP ADVAEKILRF ARAAQAVATM RGKSYLSMGS 190 200 210 220 230 240 VSMGIAGSIV NPDFFQEYLG MRNESIDLTE IIRRMAEGIY DKEEYAKAMA WTEKYCKKNE 250 260 270 280 290 300 GNDFNIPEKT KTRAQKDEDW EFIVKMTIIM RDLMQGNPKL KELGFKEEAL GHNAIAAGFQ 310 320 330 340 350 360 GQRQWTDFYP NGDFSEALLN TSFDWNGIRE AFVVTTENDA CNGVAMLFGH LLTNRAQIFS 370 380 390 400 410 420 DVRTYWSPEA VKRVTGKELT GMAANGIIHL INSGATTLDG TGQQTNANGE PAMKPCWEIT 430 440 450 460 470 480 EGEVEKCLEA TTWYPANRDY FRGGGFSSNF LSKGGMPVTM MRLNLIKGLG PVLQIAEGWT 490 500 510 520 530 540 VEIDPEIHKL LDERTDRTWP TTWFVPRLCD KPAFKDVYSV MNNWGANHGA ISYGHIGQDV 550 560 570 580 590 ITLASMLRIP VCMHNVEEDQ IFRPAAWNAF GMDKEGADYR ACTTYGPIYK

References

[1]

"Extensive DNA inversions in the B. fragilis genome control variable gene expression." Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V., Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A., Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A. , Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J., Barrell B.G., Parkhill J. Science 307:1463-1465(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25285 / NCTC 9343.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR626927 Genomic DNA. Translation: CAH05988.1.
RefSeq	YP_209950.1. NC_003228.3.
3D structure databases
HSSP	HSSP built from PDB template 1FUI based on UniProtKB P69922.
ProteinModelPortal	Q5LIN8.
ModBase	Search...
Protein-protein interaction databases
STRING	272559.BF0211.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAH05988; CAH05988; BF9343_0209.
GeneID	3286700.
KEGG	bfs:BF0211.
PATRIC	21036882. VBIBacFra29119_0207.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2407.
HOGENOM	HOG000265624.
KO	K01818.
OMA	KIGIRPT.
ProtClustDB	PRK10991.
Enzyme and pathway databases
UniPathway	UPA00563; UER00624.
Family and domain databases
Gene3D	3.20.14.10. 1 hit. 3.40.275.10. 1 hit. 3.40.50.1070. 1 hit.
HAMAP	MF_01254. Fucose_iso.
InterPro	IPR004216. Fuc/Ara_isomerase_C. IPR015888. Fuc_isomerase_C. IPR012888. Fucose_iso_N1. IPR005763. Fucose_isomerase. IPR009015. Fucose_isomerase_N/cen. IPR012889. Fucose_isomerase_N2. [Graphical view]
Pfam	PF02952. Fucose_iso_C. 1 hit. PF07881. Fucose_iso_N1. 1 hit. PF07882. Fucose_iso_N2. 1 hit. [Graphical view]
SUPFAM	SSF50443. Fuc_isomerase_C. 1 hit. SSF53743. Fuc_isomerase_N. 1 hit.
TIGRFAMs	TIGR01089. fucI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FUCI_BACFN

Accession

Primary (citable) accession number: Q5LIN8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	June 21, 2005
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program