Q5LIJ5 (MURD_BACFN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase
EC=6.3.2.9

Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Gene names

Name:	murD
Ordered Locus Names:	BF0256

Organism

Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]

Taxonomic identifier

272559 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity HAMAP MF_00639.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000108969
Regions
	Nucleotide binding	109 – 115	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5LIJ5 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: 2770589AA6093313	FASTA	444	49,577
	10 20 30 40 50 60 MKRIVVLGAG ESGAGAAVLA KVKGFDTFVS DMSAIKDKYK TLLDGHGIAW EEGRHTEEQI 70 80 90 100 110 120 LSADEVVKSP GIPNDAPLIL RLREQGTPII SEIEFAGRYT DAKMICITGS NGKTTTTSLI 130 140 150 160 170 180 YHIFKSAGLN VGLAGNIGKS LALQVAEEKH DYYVIELSSF QLDNMYNFRA DIAVLMNITP 190 200 210 220 230 240 DHLDRYDHCM QNYINAKFRI TQNQTSEDAF IFWNDDPIIK RELDKHGIRA HLYPFSAIKE 250 260 270 280 290 300 EGSIAYVEDH EVVITEPIAF NMEQEQLALT GQHNLYNSLA AGISANLAGI TKEDIRKALS 310 320 330 340 350 360 DFQGVEHRLE KVARVRGIDF INDSKATNVN SCWYALQSMT TKTVLILGGK DKGNDYTEIE 370 380 390 400 410 420 ELVREKCSAL VYLGLHNEKL HEFFDRLGLP VAEVQTGMKD AVEAAYKLAK KGETVLLSPC 430 440 CASFDLFKSY EDRGEQFKKY VREL

References

[1]

"Extensive DNA inversions in the B. fragilis genome control variable gene expression." Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V., Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A., Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A. , Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J., Barrell B.G., Parkhill J. Science 307:1463-1465(2005) [PubMed: 15746427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25285 / NCTC 9343.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR626927 Genomic DNA. Translation: CAH06031.1.
RefSeq	YP_209993.1. NC_003228.3.
3D structure databases
ProteinModelPortal	Q5LIJ5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5LIJ5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3288066.
GenomeReviews	Gene locus BF0256 in contig CR626927_GR.
KEGG	bfs:BF0256.
PATRIC	21036968. VBIBacFra29119_0249.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0771.
HOGENOM	HBG750024.
OMA	ACASWDM.
ProtClustDB	CLSK2757457.
Enzyme and pathway databases
BioCyc	BFRA272559:BF0256-MONOMER.
Family and domain databases
HAMAP	MF_00639. MurD. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01925.
PANTHER	PTHR23135:SF2. PTHR23135:SF2. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01087. MurD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_BACFN

Accession

Primary (citable) accession number: Q5LIJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
Last sequence update:	June 21, 2005
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program