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Protein

UDP-N-acetylmuramoylalanine--D-glutamate ligase

Gene

murD

Organism
Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA).UniRule annotation

Catalytic activityi

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanine + D-glutamate = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate.UniRule annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi109 – 1157ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBFRA272559:GKF0-254-MONOMER.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligaseUniRule annotation (EC:6.3.2.9UniRule annotation)
Alternative name(s):
D-glutamic acid-adding enzymeUniRule annotation
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetaseUniRule annotation
Gene namesi
Name:murDUniRule annotation
Ordered Locus Names:BF0256
OrganismiBacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343)
Taxonomic identifieri272559 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
Proteomesi
  • UP000006731 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 444444UDP-N-acetylmuramoylalanine--D-glutamate ligasePRO_0000108969Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272559.BF0256.

Structurei

3D structure databases

ProteinModelPortaliQ5LIJ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MurCDEF family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DMZ. Bacteria.
COG0771. LUCA.
HOGENOMiHOG000049428.
KOiK01925.
OMAiPGIPYTN.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.50.720. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_00639. MurD. 1 hit.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR016040. NAD(P)-bd_dom.
IPR005762. UDP-N-AcMur-Glu_ligase.
[Graphical view]
PfamiPF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01087. murD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5LIJ5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRIVVLGAG ESGAGAAVLA KVKGFDTFVS DMSAIKDKYK TLLDGHGIAW
60 70 80 90 100
EEGRHTEEQI LSADEVVKSP GIPNDAPLIL RLREQGTPII SEIEFAGRYT
110 120 130 140 150
DAKMICITGS NGKTTTTSLI YHIFKSAGLN VGLAGNIGKS LALQVAEEKH
160 170 180 190 200
DYYVIELSSF QLDNMYNFRA DIAVLMNITP DHLDRYDHCM QNYINAKFRI
210 220 230 240 250
TQNQTSEDAF IFWNDDPIIK RELDKHGIRA HLYPFSAIKE EGSIAYVEDH
260 270 280 290 300
EVVITEPIAF NMEQEQLALT GQHNLYNSLA AGISANLAGI TKEDIRKALS
310 320 330 340 350
DFQGVEHRLE KVARVRGIDF INDSKATNVN SCWYALQSMT TKTVLILGGK
360 370 380 390 400
DKGNDYTEIE ELVREKCSAL VYLGLHNEKL HEFFDRLGLP VAEVQTGMKD
410 420 430 440
AVEAAYKLAK KGETVLLSPC CASFDLFKSY EDRGEQFKKY VREL
Length:444
Mass (Da):49,577
Last modified:June 21, 2005 - v1
Checksum:i2770589AA6093313
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR626927 Genomic DNA. Translation: CAH06031.1.
RefSeqiWP_010991956.1. NC_003228.3.

Genome annotation databases

EnsemblBacteriaiCAH06031; CAH06031; BF9343_0252.
KEGGibfs:BF9343_0252.
PATRICi21036968. VBIBacFra29119_0249.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR626927 Genomic DNA. Translation: CAH06031.1.
RefSeqiWP_010991956.1. NC_003228.3.

3D structure databases

ProteinModelPortaliQ5LIJ5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272559.BF0256.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAH06031; CAH06031; BF9343_0252.
KEGGibfs:BF9343_0252.
PATRICi21036968. VBIBacFra29119_0249.

Phylogenomic databases

eggNOGiENOG4105DMZ. Bacteria.
COG0771. LUCA.
HOGENOMiHOG000049428.
KOiK01925.
OMAiPGIPYTN.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciBFRA272559:GKF0-254-MONOMER.

Family and domain databases

Gene3Di3.40.1190.10. 1 hit.
3.40.50.720. 1 hit.
3.90.190.20. 1 hit.
HAMAPiMF_00639. MurD. 1 hit.
InterProiIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR016040. NAD(P)-bd_dom.
IPR005762. UDP-N-AcMur-Glu_ligase.
[Graphical view]
PfamiPF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMiSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
TIGRFAMsiTIGR01087. murD. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMURD_BACFN
AccessioniPrimary (citable) accession number: Q5LIJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: June 21, 2005
Last modified: September 7, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.