Reviewed, UniProtKB/Swiss-Prot Q5LIJ4 (MRAY_BACFN)

Names and origin

Protein names

Recommended name:
    Phospho-N-acetylmuramoyl-pentapeptide-transferase
    EC=2.7.8.13
Alternative name(s):
    UDP-MurNAc-pentapeptide phosphotransferase

Gene names

Name:	mraY
Ordered Locus Names:	BF0257

Organism

Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]

Taxonomic identifier

272559 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan By similarity.
Catalytic activity	UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP MF_00038
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00038
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 4 family. MraY subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	phospho-N-acetylmuramoyl-pentapeptide-transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 422	422	Phospho-N-acetylmuramoyl-pentapeptide-transferase HAMAP MF_00038		PRO_0000235437
Regions
	Transmembrane	28 – 48	21	Potential
	Transmembrane	71 – 91	21	Potential
	Transmembrane	95 – 115	21	Potential
	Transmembrane	136 – 156	21	Potential
	Transmembrane	211 – 231	21	Potential
	Transmembrane	246 – 266	21	Potential
	Transmembrane	279 – 299	21	Potential
	Transmembrane	313 – 333	21	Potential
	Transmembrane	399 – 419	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5LIJ4-1 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: 3DB049C9BCD6D291	FASTA	422	46,884
	10 20 30 40 50 60 MLYYLFEWLH KLNFPGAGMF GYTSFRALMA IILALLISSI WGDKFINLLK RKQITETQRD 70 80 90 100 110 120 AKIDPFGVNK VGVPSMGGVI IIVAILIPCL LLGKLHNIYM ILMLITTVWL GSLGFADDYI 130 140 150 160 170 180 KIFKKDKEGL HGKFKIIGQV GLGLIVGLTL YLSPDVVIRE NIEVQKSENE IEVIHGTHDL 190 200 210 220 230 240 KSTQTTIPFF KSNNLDYADL VGFMGEHAQT AGWILFVIIT IFVVTAVSNG ANLNDGMDGM 250 260 270 280 290 300 AAGNSAIIGL TLGILAYVSS HIEFAGYLNI MYIPGSEELV IFICAFIGAL IGFLWYNAYP 310 320 330 340 350 360 AQVFMGDTGS LTIGGIIAVF AIIIHKELLI PILCGIFLVE NLSVLLQRFY YKAGKRKGIK 370 380 390 400 410 420 QRLFKRAPIH DHFRTSMSLV EPGCSVKFTK PDQLFHESKI TVRFWIVTIV LAAITIITLK IR

References

[1]

"Extensive DNA inversions in the B. fragilis genome control variable gene expression." Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V., Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A., Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A. , Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J., Barrell B.G., Parkhill J. Science 307:1463-1465(2005) [PubMed: 15746427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR626927 Genomic DNA. Translation: CAH06032.1.
RefSeq	YP_209994.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5LIJ4.
Genome annotation databases
GeneID	3289246.
GenomeReviews	Gene locus BF0257 in contig CR626927_GR.
KEGG	bfs:BF0257.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5LIJ4.
OMA	HHHFEQK.
Enzyme and pathway databases
BioCyc	BFRA272559:BF0257-MON.
Family and domain databases
HAMAP	MF_00038. [Tree]
InterPro	IPR000715. Glycosyl_transferase_4. IPR018481. Glycosyl_Trfase_4_cons-reg. IPR003524. PNAcMuramoyl-5peptid_Trfase. IPR018480. PNAcMuramoyl-5peptid_Trfase_CS. [Graphical view]
PANTHER	PTHR22926. Glyco_trans_4. 1 hit. PTHR22926:SF3. PNAcPpept_trans. 1 hit.
Pfam	PF00953. Glycos_transf_4. 1 hit. PF10555. MraY_sig1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00445. mraY. 1 hit.
PROSITE	PS01347. MRAY_1. 1 hit. PS01348. MRAY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MRAY_BACFN

Accession

Primary (citable) accession number: Q5LIJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	June 21, 2005
Last modified:	November 3, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)