Reviewed, UniProtKB/Swiss-Prot Q5LIJ3 (MURE_BACFN)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
    EC=6.3.2.13
Alternative name(s):
    UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
    Meso-diaminopimelate-adding enzyme
    Meso-A2pm-adding enzyme
    UDP-N-acetylmuramyl-tripeptide synthetase
    UDP-MurNAc-tripeptide synthetase

Gene names

Name:	murE
Ordered Locus Names:	BF0258

Organism

Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]

Taxonomic identifier

272559 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	485 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity. HAMAP MF_00208
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP MF_00208
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00208
Subcellular location	Cytoplasm By similarity HAMAP MF_00208.
Post-translational modification	Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity. HAMAP MF_00208
Sequence similarities	Belongs to the murCDEF family. MurE subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 485	485	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP MF_00208		PRO_1000012337
Regions
	Nucleotide binding	111 – 117	7	ATP Potential
	Region	153 – 154	2	UDP-MurNAc-L-Ala-D-Glu binding By similarity
	Region	402 – 405	4	Meso-diaminopimelate binding By similarity
	Motif	402 – 405	4	Meso-diaminopimelate recognition motif HAMAP MF_00208
Sites
	Binding site	30	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	180	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	186	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	188	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	378	1	Meso-diaminopimelate By similarity
	Binding site	455	1	Meso-diaminopimelate; via carbonyl oxygen By similarity
	Binding site	459	1	Meso-diaminopimelate By similarity
Amino acid modifications
	Modified residue	220	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5LIJ3-1 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: 27D490E6A7CAA51C	FASTA	485	53,195
	10 20 30 40 50 60 MKLKEILTSI QPVKITGNQD IEITGVDIDS RQVESGHLFM AMHGTQTDGH AYIPAAVEKG 70 80 90 100 110 120 ATAILCEELP AELAEGVTYI QVADSEDAVG KAATTFYGNP SSKLELVGVT GTNGKTTIAT 130 140 150 160 170 180 LLYNTFRYFG YKVGLISTVC NYIDDEAIPT EHTTPDPITL NRLLGRMADE GCKYVFMEVS 190 200 210 220 230 240 SHSIAQKRIS GLRFAGGIFT NLTRDHLDYH KTVENYLKAK KKFFDDMPKN SFSLTNLDDK 250 260 270 280 290 300 NGLVMTQNTK SKVYTYSLRS LSDFKGRVLE SHFEGMLLDF NNHELAVQFI GKFNASNLLA 310 320 330 340 350 360 VFGAAVLLGK KEEDVLVALS TLHPVAGRFD AIRSPQGYTA IVDYAHTPDA LVNVLNAIHG 370 380 390 400 410 420 VLEGKGKVIT VVGAGGNRDK GKRPIMAKEA ARASDRVIIT SDNPRFEEPQ DIINDMLAGL 430 440 450 460 470 480 DTEDKKKTLS IADRKEAIRT ACMLAEKGDV ILVAGKGHEN YQDIKGVKHH FDDKEVLKEI FSLTV

References

[1]

"Extensive DNA inversions in the B. fragilis genome control variable gene expression." Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V., Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A., Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A. , Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J., Barrell B.G., Parkhill J. Science 307:1463-1465(2005) [PubMed: 15746427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR626927 Genomic DNA. Translation: CAH06033.1.
RefSeq	YP_209995.1.
3D structure databases
HSSP	HSSP built from PDB template 1E8C based on UniProtKB P22188.
SMR	Q5LIJ3. Positions 2-482.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5LIJ3.
Genome annotation databases
GeneID	3289272.
GenomeReviews	Gene locus BF0258 in contig CR626927_GR.
KEGG	bfs:BF0258.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0769.
HOGENOM	HBG602753.
OMA	EHLDFHG.
Enzyme and pathway databases
BioCyc	BFRA272559:BF0258-MONOMER.
Family and domain databases
HAMAP	MF_00208. MurE. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01085. murE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURE_BACFN

Accession

Primary (citable) accession number: Q5LIJ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 21, 2005
Last modified:	February 9, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)