Q5LIH9 (DUT_BACFN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Short name=dUTPase
EC=3.6.1.23

Alternative name(s):
dUTP pyrophosphatase

Gene names

Name:	dut
Ordered Locus Names:	BF0272

Organism

Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]

Taxonomic identifier

272559 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	144 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP MF_00116
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP MF_00116
Cofactor	Magnesium By similarity. HAMAP MF_00116
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP MF_00116
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	dUTP diphosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 144	144	Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP MF_00116		PRO_0000231398
Regions
	Region	63 – 65	3	Substrate binding By similarity
	Region	80 – 82	3	Substrate binding By similarity
Sites
	Binding site	76	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5LIH9 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: 3E95AFABE831686F	FASTA	144	15,608
	10 20 30 40 50 60 MNIQVINKSK HPLPAYATEL SAGMDIRANI SEPISLAPMQ RCLVPTGLFI ALPQGFEAQI 70 80 90 100 110 120 RPRSGLALKK GITVLNSPGT IDADYRGEIC IILVNLSAET FVIEDGERIA QMVIARHEQA 130 140 VWKEVEVLDE TERGAGGFGH TGRG

References

[1]

"Extensive DNA inversions in the B. fragilis genome control variable gene expression." Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V., Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A., Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A. , Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J., Barrell B.G., Parkhill J. Science 307:1463-1465(2005) [PubMed: 15746427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25285 / NCTC 9343.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR626927 Genomic DNA. Translation: CAH06047.1.
RefSeq	YP_210009.1. NC_003228.3.
3D structure databases
ProteinModelPortal	Q5LIH9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5LIH9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3285475.
GenomeReviews	Gene locus BF0272 in contig CR626927_GR.
KEGG	bfs:BF0272.
PATRIC	21037000. VBIBacFra29119_0265.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0756.
HOGENOM	HBG436079.
OMA	AQMVFAP.
ProtClustDB	PRK00601.
Enzyme and pathway databases
BioCyc	BFRA272559:BF0272-MONOMER.
Family and domain databases
HAMAP	MF_00116. dUTPase_bact. [Tree]
InterPro	IPR008180. dUTP_pyroPase. IPR008181. dUTP_pyroPase_sf. [Graphical view]
KO	K01520.
PANTHER	PTHR11241. PTHR11241. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. Dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_BACFN

Accession

Primary (citable) accession number: Q5LIH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	June 21, 2005
Last modified:	January 25, 2012
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program