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Reviewed, UniProtKB/Swiss-Prot Q5LI95 (NRFA_BACFN)

Last modified November 3, 2009. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cytochrome c-552
    EC=1.7.2.2
Alternative name(s):
    Ammonia-forming cytochrome c nitrite reductase
      Short name=Cytochrome c nitrite reductase
Gene names
Name: nrfA
Ordered Locus Names: BF0361
OrganismBacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]
Taxonomic identifier272559 [NCBI]
Taxonomic lineageBacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

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Protein attributes

Sequence length493 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Plays a role in nitrite reduction By similarity.

Catalytic activity

NH3 + 2 H2O + 6 ferricytochrome c = nitrite + 6 ferrocytochrome c + 7 H+. HAMAP MF_01182

Cofactor

Binds 1 calcium ion per monomer By similarity.

Binds 5 heme groups covalently per monomer By similarity.

Pathway

Nitrogen metabolism; nitrate reduction (assimilation). HAMAP MF_01182

Subcellular location

Periplasm By similarity.

Sequence similarities

Belongs to the cytochrome c-552 family.

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Ontologies

Keywords
   Biological processElectron transport
Transport
   Cellular componentPeriplasm
   DomainSignal
   LigandCalcium
Heme
Iron
Metal-binding
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processelectron transport chain

Inferred from electronic annotation. Source: UniProtKB-KW

nitrogen compound metabolic process

Inferred from electronic annotation. Source: HAMAP

transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentperiplasmic space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

heme binding

Inferred from electronic annotation. Source: InterPro

nitrite reductase (cytochrome, ammonia-forming) activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 493468Cytochrome c-552 HAMAP MF_01182
PRO_0000268959

Sites

Metal binding1161Iron (heme 3 axial ligand) By similarity
Metal binding1481Iron (heme 1 axial ligand) By similarity
Metal binding1861Iron (heme 2 axial ligand) By similarity
Metal binding2281Iron (heme 3 axial ligand) By similarity
Metal binding2301Calcium By similarity
Metal binding2311Calcium; via carbonyl oxygen By similarity
Metal binding2761Calcium; via carbonyl oxygen By similarity
Metal binding2781Calcium By similarity
Metal binding2901Iron (heme 5 axial ligand) By similarity
Metal binding3011Iron (heme 4 axial ligand) By similarity
Metal binding3151Iron (heme 2 axial ligand) By similarity
Metal binding3321Iron (heme 5 axial ligand) By similarity
Metal binding4071Iron (heme 4 axial ligand) By similarity
Binding site1441Heme 1 (covalent) By similarity
Binding site1471Heme 1 (covalent) By similarity
Binding site1821Heme 2 (covalent) By similarity
Binding site1851Heme 2 (covalent) By similarity
Binding site2241Heme 3 (covalent) By similarity
Binding site2271Heme 3 (covalent) By similarity
Binding site2311Substrate By similarity
Binding site2791Substrate By similarity
Binding site2971Heme 4 (covalent) By similarity
Binding site3001Heme 4 (covalent) By similarity
Binding site3281Heme 5 (covalent) By similarity
Binding site3311Heme 5 (covalent) By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q5LI95-1 [UniParc].

Last modified June 21, 2005. Version 1.
Checksum: C99534C2982DC16C

FASTA49356,068
        10         20         30         40         50         60 
MEKKLKSWQG WLLFCGAMAV VFVLGLVVSS LMERRAETVS VFNNKRVEIT GIEARNEVFG 

        70         80         90        100        110        120 
ENYPRQYETW KETAKTDFKS EFNGNEAVDV LEQRPEMVVL WAGYAFSKDY STPRGHMHAI 

       130        140        150        160        170        180 
EDITHSLRTG APMDDKSGPQ PSTCWTCKSP DVPRMMEAIG VDSFYNNKWG AFGSEIVNPI 

       190        200        210        220        230        240 
GCADCHEPTN MKLHISRPAL REAFARQGKD IDKATPQEMR SLVCAQCHVE YYFKGDGKYL 

       250        260        270        280        290        300 
TFPWDKGFSV EDMEAYYDEA DFADYTHALS KARILKAQHP DYEISQMGIH AQRGVSCADC 

       310        320        330        340        350        360 
HMPYKSEGGM KFSDHHIQSP LAMIDRTCQV CHRESEETLR NNVYDRQRKA NEIRGRLEQE 

       370        380        390        400        410        420 
LAKAHIEAKF AWDKGATDVQ MAEALKLIRQ AQWRWDFGVA SHGGAFHAPQ EIQRILGHGL 

       430        440        450        460        470        480 
DKALQARLAI SKVLAQHGYT ADVPMPDIST KEKAQEYIGL DMEKERKAKD KFLKTIVPEW 

       490 
LEKARANGRL AKL

« Hide

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References

[1]"Extensive DNA inversions in the B. fragilis genome control variable gene expression."
Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V., Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A., Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A. expand/collapse author list , Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J., Barrell B.G., Parkhill J.
Science 307:1463-1465(2005) [PubMed: 15746427] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

CR626927 Genomic DNA. Translation: CAH06131.1.
RefSeqYP_210093.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ5LI95.

Genome annotation databases

GeneID3289172.
GenomeReviewsGene locus BF0361 in contig CR626927_GR.
KEGGbfs:BF0361.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ5LI95.
OMARDINSAC.

Enzyme and pathway databases

BioCycBFRA272559:BF0361-MON.

Family and domain databases

HAMAPMF_01182.
[Tree]
InterProIPR003321. Cyt_c552.
IPR011031. Multihaem_cyt.
[Graphical view]
PfamPF02335. Cytochrom_C552. 1 hit.
[Graphical view]
PIRSFPIRSF000243. Cyt_c552. 1 hit.
PROSITEPS51008. MULTIHEME_CYTC. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameNRFA_BACFN
AccessionPrimary (citable) accession number: Q5LI95
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: June 21, 2005
Last modified: November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents