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Q5LI31 (DDL_BACFN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:BF0435
OrganismBacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]
Taxonomic identifier272559 [NCBI]
Taxonomic lineageBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides

Protein attributes

Sequence length324 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 324324D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000030424

Regions

Domain121 – 321201ATP-grasp
Nucleotide binding149 – 20456ATP By similarity

Sites

Metal binding2751Magnesium or manganese 1 By similarity
Metal binding2881Magnesium or manganese 1 By similarity
Metal binding2881Magnesium or manganese 2 By similarity
Metal binding2901Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5LI31 [UniParc].

Last modified June 21, 2005. Version 1.
Checksum: 514769F1E9649728

FASTA32436,076
        10         20         30         40         50         60 
MKRNIAIVAG GDTSEIVVSL RSAQGIYSFI DKEKYNLYIV EMEGRRWEVQ LQDGSKTPVD 

        70         80         90        100        110        120 
RNDFSFMNGA EKVVFDFAYI TIHGTPGEDG RLQGYFDMIR IPYSCCGVLA AAITYDKFVC 

       130        140        150        160        170        180 
NQYLKAFGVR ISESLLLRQG QAVSDEDVVE KIGLPCFIKP NLGGSSFGVT KVKTREQIQP 

       190        200        210        220        230        240 
AIAKAFSEAE EVMIEAFMGG TELTCGCYKT KEKSVVFPLT EVVTHNEFFD YDAKYNGQVD 

       250        260        270        280        290        300 
EITPARISEE LTRRVQTLTS AIYDILGCSG IIRVDYIITE GEKINLLEVN TTPGMTATSF 

       310        320 
IPQQVRAAGL DIKDVMTDII ENKF 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR626927 Genomic DNA. Translation: CAH06197.1.
RefSeqYP_210157.1. NC_003228.3.

3D structure databases

ProteinModelPortalQ5LI31.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272559.BF0435.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAH06197; CAH06197; BF9343_0418.
GeneID3285190.
KEGGbfs:BF0435.
PATRIC21037332. VBIBacFra29119_0430.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMADTQYRIP.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycBFRA272559:GKF0-421-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_BACFN
AccessionPrimary (citable) accession number: Q5LI31
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: June 21, 2005
Last modified: February 19, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways