Q5LI12 (PYRE_BACFN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Orotate phosphoribosyltransferase
Short name=OPRT
Short name=OPRTase
EC=2.4.2.10

Gene names

Name:	pyrE
Ordered Locus Names:	BF0455

Organism

Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]

Taxonomic identifier

272559 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208
Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208
Cofactor	Magnesium By similarity. HAMAP MF_01208
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208
Subunit structure	Homodimer By similarity. HAMAP MF_01208
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Magnesium
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 212	212	Orotate phosphoribosyltransferase HAMAP MF_01208		PRO_1000066202
Regions
	Region	123 – 131	9	5-phosphoribose 1-diphosphate binding By similarity
Sites
	Binding site	97	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	101	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	103	1	5-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
	Binding site	127	1	Orotate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5LI12 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: 8EA9C26C7381C8CD	FASTA	212	23,504
	10 20 30 40 50 60 MKNLERLFAE KLLKIKAIKL QPANPFTWAS GWKSPFYCDN RKTLSYPSLR SFVKFEITRL 70 80 90 100 110 120 VLERFGQVDA IAGVATGAIP QGALVADALN LPFVYVRSTP KDHGLENLIE GELRPGMKVV 130 140 150 160 170 180 VVEDLISTGG SSLKAVEAIR RDGCEVIGMV AAYTYGFPVA EQAFKDAKVP LVTLTNYEAV 190 200 210 LDVALRTGYI EEEDIATLNE WRKDPAHWET GK

References

[1]

"Extensive DNA inversions in the B. fragilis genome control variable gene expression." Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V., Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A., Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A. , Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J., Barrell B.G., Parkhill J. Science 307:1463-1465(2005) [PubMed: 15746427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25285 / NCTC 9343.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR626927 Genomic DNA. Translation: CAH06216.1.
RefSeq	YP_210176.1. NC_003228.3.
3D structure databases
HSSP	HSSP built from PDB template 1ZN8 based on UniProtKB P07741.
ProteinModelPortal	Q5LI12.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5LI12.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3289317.
GenomeReviews	Gene locus BF0455 in contig CR626927_GR.
KEGG	bfs:BF0455.
PATRIC	21037372. VBIBacFra29119_0450.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0461.
HOGENOM	HBG404341.
OMA	LPMTYVR.
ProtClustDB	PRK00455.
Enzyme and pathway databases
BioCyc	BFRA272559:BF0455-MONOMER.
Family and domain databases
HAMAP	MF_01208. PyrE. [Tree]
InterPro	IPR004467. Or_phspho_trans_clade-1. IPR023031. Orotate_PribosylTferase. IPR000836. PRibTrfase. [Graphical view]
KO	K00762.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR00336. PyrE. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRE_BACFN

Accession

Primary (citable) accession number: Q5LI12

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	June 21, 2005
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program