Q5LHG9 (AROA_BACFN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	BF0666

Organism

Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]

Taxonomic identifier

272559 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 410	410	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_1000099665

Sequences

	Sequence		Length	Mass (Da)
	Q5LHG9 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: A8A6CE6B2BEDB864	FASTA	410	45,632
	10 20 30 40 50 60 MRYLLSAPSQ IKATIQLPAS KSISNRALII HALSKGDDVP SNLSDCDDTQ VMIKALTEGN 70 80 90 100 110 120 EVIDILAAGT AMRFLTAYLS STPGIHTITG TERMQQRPIQ ILVNALRELG AHIEYVRNEG 130 140 150 160 170 180 FPPLRIEGRE LTGSEITLKG NVSSQYISAL LMIGPVLKNG LQLRLTGEIV SRPYINLTLQ 190 200 210 220 230 240 LMKDFGASAS WTSDQNIQVD PQPYHCLPFT VESDWSAASY WYQIAALSPQ ADIELTGLFR 250 260 270 280 290 300 HSYQGDSRGA EVFARLGVAT EYTETGIRLK KNGTCVERLD EDFVDIPDLA QTFVVTCALL 310 320 330 340 350 360 NVPFRFTGLQ SLKIKETDRI EALKTEMKKL GYILHDKNDS ILSWDGERVE QQTCPVIKTY 370 380 390 400 410 EDHRMAMAFA PAAIHYPTIQ IDEPQVVSKS YPGYWDDLRK AGFGIKVGEE

References

[1]

"Extensive DNA inversions in the B. fragilis genome control variable gene expression." Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V., Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A., Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A. , Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J., Barrell B.G., Parkhill J. Science 307:1463-1465(2005) [PubMed: 15746427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25285 / NCTC 9343.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR626927 Genomic DNA. Translation: CAH06411.1.
RefSeq	YP_210369.1. NC_003228.3.
3D structure databases
HSSP	HSSP built from PDB template 1P89 based on UniProtKB P0A6D3.
ProteinModelPortal	Q5LHG9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5LHG9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3289127.
GenomeReviews	Gene locus BF0666 in contig CR626927_GR.
KEGG	bfs:BF0666.
PATRIC	21037790. VBIBacFra29119_0653.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0128.
HOGENOM	HBG646626.
OMA	FADHRIA.
ProtClustDB	CLSK2757416.
Enzyme and pathway databases
BioCyc	BFRA272559:BF0666-MONOMER.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 3 hits.
KO	K00800.
Pfam	PF00275. EPSP_synthase. 2 hits. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. False negative. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_BACFN

Accession

Primary (citable) accession number: Q5LHG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 21, 2005
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program