Q5LH14 (LPXD_BACFN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	BF0829

Organism

Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]

Taxonomic identifier

272559 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_0000264347
Sites
	Active site	240	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5LH14 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: 8973DA80A3E4F4C6	FASTA	346	37,108
	10 20 30 40 50 60 MEFSAKQIAA FIQGEIIGDE NATVHTFAKI EEGIPGAISF LSNPKYTPYI YETKASIVLV 70 80 90 100 110 120 NKDFTPEQEV KATLIKVDNA YESLAKLLNL YEMSKPKRTG IDERAYVAET AKIGKDVYIA 130 140 150 160 170 180 PFACIGDHAE VGDNTVIHPH ATVGGGAKIG SNCILYANST VYHDCRVGNN CILHAGCVIG 190 200 210 220 230 240 ADGFGFAPTP QGYEKIPQIG IVILEDNVEV GANTCIDRAT MGATVIHSGV KLDNLVQIAH 250 260 270 280 290 300 NDEIGSHTVM AAQVGIAGST KVGEWCMFGG QVGIAGHLKI GNQVNLGAQS GVPGNIKSGS 310 320 330 340 QLIGTPPMEL KQFFKASIVQ KSLPEMQIEL RNLRKEIEEL KQQLNK

References

[1]

"Extensive DNA inversions in the B. fragilis genome control variable gene expression." Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V., Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A., Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A. , Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J., Barrell B.G., Parkhill J. Science 307:1463-1465(2005) [PubMed: 15746427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25285 / NCTC 9343.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR626927 Genomic DNA. Translation: CAH06572.1.
RefSeq	YP_210524.1. NC_003228.3.
3D structure databases
ProteinModelPortal	Q5LH14.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5LH14.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3289227.
GenomeReviews	Gene locus BF0829 in contig CR626927_GR.
KEGG	bfs:BF0829.
PATRIC	21038128. VBIBacFra29119_0820.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1044.
HOGENOM	HBG469615.
OMA	SYPAKIM.
ProtClustDB	PRK00892.
Enzyme and pathway databases
BioCyc	BFRA272559:BF0829-MONOMER.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 4 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_BACFN

Accession

Primary (citable) accession number: Q5LH14

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	June 21, 2005
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program