Reviewed, UniProtKB/Swiss-Prot Q5LH03 (PUR7_BACFN)

Names and origin

Protein names

Recommended name:
    Phosphoribosylaminoimidazole-succinocarboxamide synthase
    EC=6.3.2.6
Alternative name(s):
    SAICAR synthetase

Gene names

Name:	purC
Ordered Locus Names:	BF0840

Organism

Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]

Taxonomic identifier

272559 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate. HAMAP MF_00137
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2. HAMAP MF_00137
Sequence similarities	Belongs to the SAICAR synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazolesuccinocarboxamide synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Phosphoribosylaminoimidazole-succinocarboxamide synthase HAMAP MF_00137		PRO_1000018667

Sequences

	Sequence		Length	Mass (Da)
	Q5LH03-1 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: 971712767111A585	FASTA	314	35,569
	10 20 30 40 50 60 MKALTKTDFN FPGQKSVYHG KVRDVYNING EQLVMVATDR ISAFDVVLPE GIPYKGQMLN 70 80 90 100 110 120 QIAAKFLDAT TDICPNWKLA TPDPMVTVGV LCEGFPVEMI VRGYLCGSAW RAYKNGVREI 130 140 150 160 170 180 CGVKLPEGMK ENQKFPEPIV TPTTKAEMGL HDEDISKEEI LAQGLATPEE YAILEKYTLA 190 200 210 220 230 240 LFKRGTEIAA ERGLILVDTK YEFGKHNGTI YLMDEIHTPD SSRYFYAEGY QERFEKGEAQ 250 260 270 280 290 300 KQLSKEFVRE WLMENGFQGK EGQKVPEMTP AIVESISERY IELFENITGE KFVKEDTSNI 310 AERIEKNVMA FLAK

References

[1]

"Extensive DNA inversions in the B. fragilis genome control variable gene expression." Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V., Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A., Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A. , Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J., Barrell B.G., Parkhill J. Science 307:1463-1465(2005) [PubMed: 15746427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR626927 Genomic DNA. Translation: CAH06583.1.
RefSeq	YP_210535.1.
3D structure databases
HSSP	HSSP built from PDB template 1OBD based on UniProtKB P27616.
SMR	Q5LH03. Positions 3-293.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5LH03.
Genome annotation databases
GeneID	3288527.
GenomeReviews	Gene locus BF0840 in contig CR626927_GR.
KEGG	bfs:BF0840.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0152.
HOGENOM	HBG555040.
OMA	LTLMDEV.
Enzyme and pathway databases
BioCyc	BFRA272559:BF0840-MONOMER.
Family and domain databases
HAMAP	MF_00137. SAICAR_synth. [Tree]
InterPro	IPR013816. ATP_grasp_subdomain_2. IPR001636. SAICAR_synt. IPR018236. SAICAR_synthetase_CS. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
PANTHER	PTHR11609. SAICAR_synt. 1 hit.
Pfam	PF01259. SAICAR_synt. 1 hit. [Graphical view]
PROSITE	PS01057. SAICAR_SYNTHETASE_1. False negative. PS01058. SAICAR_SYNTHETASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR7_BACFN

Accession

Primary (citable) accession number: Q5LH03

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 21, 2005
Last modified:	January 19, 2010
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)