Q5LGZ8 (GLAB_BACFN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1,3-galactosidase B
EC=3.2.1.n1
EC=3.2.1.n2

Alternative name(s):
BfGal110B
Exo-alpha-galactosidase B
EC=3.2.1.22

Gene names

Name:	glaB
Synonyms:	fragA
Ordered Locus Names:	BF0845

Organism

Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]

Taxonomic identifier

272559 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	595 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Alpha-galactosidase. Removes both branched alpha-1,3-linked galactose residues of blood group B antigens and linear alpha-1,3-linked galactose structures. Ref.1
Catalytic activity	Hydrolysis of terminal, non-reducing branched (1->3)-alpha-D-galactosidic residues, producing free D-galactose. Ref.1 Ref.3 Hydrolysis of terminal, non-reducing linear (1->3)-alpha-D-galactosidic residues, producing free D-galactose. Ref.1 Ref.3 Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. Ref.1 Ref.3
Sequence similarities	Belongs to the glycosyl hydrolase 110 family. B subfamily. Contains 3 PbH1 repeats.
Biophysicochemical properties	Kinetic parameters: KM=0.7 mM for Galalpha-pNP Ref.1 pH dependence: Optimum pH is 5-7.5.

Ontologies

Keywords
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	raffinose alpha-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 595	573	Alpha-1,3-galactosidase B		PRO_0000348476
Regions
	Repeat	432 – 454	23	PbH1 1
	Repeat	455 – 477	23	PbH1 2
	Repeat	488 – 541	54	PbH1 3

Sequences

	Sequence		Length	Mass (Da)
	Q5LGZ8 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: F5EE6940D35877A5	FASTA	595	67,011
	10 20 30 40 50 60 MKTILLFALS LLLSLSVSDV CAQERVYDIS QFGLKANSKK NASPVVRKAI AKIKAECRDG 70 80 90 100 110 120 EKVILRFPAG RYNFHEAGST VREYYISNHD QDNPKKVGIA LEDMKNLTID GQGSEFVFYG 130 140 150 160 170 180 RMIPVSLLRS ENCVLKNFSI DFEQPHIAQV QVVENDPEKG ITFEPAPWVD YRISKDSVFE 190 200 210 220 230 240 GLGEGWVMRY SWGIAFDGKT KHVVYNTSDI GCPTKGAFEV APRRICSPKW KDARLVPGTV 250 260 270 280 290 300 VAMRGWGRPT PGIFMSHDVN TSLLDVKVHY AEGMGLLAQL CEDITLDGFG VCLKGDNDPR 310 320 330 340 350 360 YFTTQADATH FSGCKGKIVS KNGLYEGMMD DAINVHGTYL KVIKRVDDHT LIGRYMHDQS 370 380 390 400 410 420 WGFEWGRPGD DVQFVRSETM ELIGKQNQIT AIRPYDKGEI RGAREFSITF KEAIDPAINE 430 440 450 460 470 480 KSGFGIENLT WTPEVLFAGN TIRNNRARGT LFSTPKKTVV EDNLFDHTSG TAILLCGDCN 490 500 510 520 530 540 GWFETGACRD VTIRRNRFIN ALTNMFQFTN AVISIYPEIP NLKDQQKYFH GGKDGGIVIE 550 560 570 580 590 DNEFDTFDAP ILYAKSVDGL IFRNNVIKTN TEFKPFHWNK DRFLLERVTN VKISE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bacterial glycosidases for the production of universal red blood cells." Liu Q.P., Sulzenbacher G., Yuan H., Bennett E.P., Pietz G., Saunders K., Spence J., Nudelman E., Levery S.B., White T., Neveu J.M., Lane W.S., Bourne Y., Olsson M.L., Henrissat B., Clausen H. Nat. Biotechnol. 25:454-464(2007) [PubMed: 17401360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
[2]	"Extensive DNA inversions in the B. fragilis genome control variable gene expression." Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V., Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A., Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A. , Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J., Barrell B.G., Parkhill J. Science 307:1463-1465(2005) [PubMed: 15746427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25285 / NCTC 9343.
[3]	"Identification of a GH110 subfamily of alpha1,3-galactosidases: novel enzymes for removal of the alpha3Gal xenotransplantation antigen." Liu Q.P., Yuan H., Bennett E.P., Levery S.B., Nudelman E., Spence J., Pietz G., Saunders K., White T., Olsson M.L., Henrissat B., Sulzenbacher G., Clausen H. J. Biol. Chem. 283:8545-8554(2008) [PubMed: 18227066] [Abstract] Cited for: ENZYME ACTIVITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM109955 Genomic DNA. Translation: CAJ33351.1. CR626927 Genomic DNA. Translation: CAH06588.1.
RefSeq	YP_210540.1. NC_003228.3.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5LGZ8.
Protein family/group databases
CAZy	GH110. Glycoside Hydrolase Family 110.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3288660.
GenomeReviews	Gene locus BF0845 in contig CR626927_GR.
KEGG	bfs:BF0845.
PATRIC	21038158. VBIBacFra29119_0835.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	NOG77539.
HOGENOM	HBG345842.
OMA	LTWTPEV.
ProtClustDB	CLSK823424.
Enzyme and pathway databases
BioCyc	BFRA272559:BF0845-MONOMER.
Family and domain databases
InterPro	IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 3 hits.
SMART	SM00710. PbH1. 3 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLAB_BACFN

Accession

Primary (citable) accession number: Q5LGZ8
Secondary accession number(s): A4Q8G6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	June 21, 2005
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program