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Q5LGU6

- LIPA_BACFN

UniProt

Q5LGU6 - LIPA_BACFN

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Protein
Lipoyl synthase
Gene
lipA, BF0898
Organism
Bacteroides fragilis (strain ATCC 25285 / NCTC 9343)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi39 – 391Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi44 – 441Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi50 – 501Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi65 – 651Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi69 – 691Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi72 – 721Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBFRA272559:GKF0-873-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:BF0898
OrganismiBacteroides fragilis (strain ATCC 25285 / NCTC 9343)
Taxonomic identifieri272559 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
ProteomesiUP000006731: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 288288Lipoyl synthaseUniRule annotation
PRO_0000325234Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272559.BF0898.

Structurei

3D structure databases

ProteinModelPortaliQ5LGU6.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiPNIAECF.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5LGU6-1 [UniParc]FASTAAdd to Basket

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MGNDKRVRKP EWLKISIGAN ERYTETKRIV ESHCLHTICS SGRCPNMGEC    50
WGKGTATFMI AGDICTRSCK FCNTQTGRPL PLDPDEPTHV AESIALMKLS 100
HAVITSVDRD DLPDLGAAHW AQTIREIKRL NPETTTEVLI PDFQGRKELV 150
DQVIKACPEI ISHNMETVKR ISPQVRSAAN YHTSLEVIRQ IAESGITAKS 200
GIMVGLGETP AEVEELMDDL ISVGCKILTI GQYLQPTHKH FPVAAYITPE 250
QFAVYKETGL KKGFEQVESA PLVRSSYHAE KHIRFNNK 288
Length:288
Mass (Da):32,108
Last modified:June 21, 2005 - v1
Checksum:i48A0ED0A235CB9C3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR626927 Genomic DNA. Translation: CAH06641.1.
RefSeqiYP_210592.1. NC_003228.3.

Genome annotation databases

EnsemblBacteriaiCAH06641; CAH06641; BF9343_0860.
GeneIDi3288526.
KEGGibfs:BF0898.
PATRICi21038266. VBIBacFra29119_0887.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR626927 Genomic DNA. Translation: CAH06641.1 .
RefSeqi YP_210592.1. NC_003228.3.

3D structure databases

ProteinModelPortali Q5LGU6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272559.BF0898.

Protocols and materials databases

DNASUi 3288526.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAH06641 ; CAH06641 ; BF9343_0860 .
GeneIDi 3288526.
KEGGi bfs:BF0898.
PATRICi 21038266. VBIBacFra29119_0887.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi PNIAECF.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci BFRA272559:GKF0-873-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25285 / NCTC 9343.

Entry informationi

Entry nameiLIPA_BACFN
AccessioniPrimary (citable) accession number: Q5LGU6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: June 21, 2005
Last modified: May 14, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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