Q5LFT6 (GCST_BACFN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aminomethyltransferase
EC=2.1.2.10

Alternative name(s):
Glycine cleavage system T protein

Gene names

Name:	gcvT
Ordered Locus Names:	BF1290

Organism

Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]

Taxonomic identifier

272559 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine By similarity. HAMAP MF_00259
Catalytic activity	[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3. HAMAP MF_00259
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Sequence similarities	Belongs to the GcvT family.

Ontologies

Keywords
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	aminomethyltransferase activity Inferred from electronic annotation. Source: EC transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 361	361	Aminomethyltransferase HAMAP MF_00259		PRO_1000047645

Sequences

	Sequence		Length	Mass (Da)
	Q5LFT6 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: E5E9D8FE6A152D8C	FASTA	361	39,870
	10 20 30 40 50 60 MKTTPFTEKH IALGAKMHEF AGYNMPIEYS GIIDEHLTVC NGVGVFDVSH MGEFWVKGPH 70 80 90 100 110 120 ALDFLQKVTS NNVAALVPGK IQYTCFPNED GGIVDDLLVY QYEPEKYLLV VNASNIEKDW 130 140 150 160 170 180 NWCISHNTEG AELENSSDNM AQLAVQGPKA IQALQKLTDI NLADIPYYTF KVGEFAGEKN 190 200 210 220 230 240 VIISNTGYTG AGGFELYFYP DAAMKIWDAV FEAGAEFGIK PIGLGARDTL RLEMGFCLYG 250 260 270 280 290 300 NDLDDTTSPI EAGLGWITKF VDGKNFTNRS MLEKQKAEGT VRKLVGFEMI DRGIPRHGYE 310 320 330 340 350 360 LTTAEGDKIG VVTSGTMSPI RKIGIGMGYV KPEYSKIGTE ICIDMRGRKL KAVVVKPPFR K

References

[1]

"Extensive DNA inversions in the B. fragilis genome control variable gene expression." Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V., Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A., Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A. , Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J., Barrell B.G., Parkhill J. Science 307:1463-1465(2005) [PubMed: 15746427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25285 / NCTC 9343.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR626927 Genomic DNA. Translation: CAH07007.1.
RefSeq	YP_210952.1. NC_003228.3.
3D structure databases
HSSP	HSSP built from PDB template 1WOS based on UniProtKB Q9WY54.
ProteinModelPortal	Q5LFT6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5LFT6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3286680.
GenomeReviews	Gene locus BF1290 in contig CR626927_GR.
KEGG	bfs:BF1290.
PATRIC	21039026. VBIBacFra29119_1265.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0404.
HOGENOM	HBG299834.
OMA	KALYGGM.
ProtClustDB	PRK00389.
Enzyme and pathway databases
BioCyc	BFRA272559:BF1290-MONOMER.
Family and domain databases
HAMAP	MF_00259. GcvT. [Tree]
InterPro	IPR013977. GCV_T_C. IPR006222. GCV_T_N. IPR006223. GcvT. IPR022903. NH2_Me_Trfase. [Graphical view]
KO	K00605.
PANTHER	PTHR13847:SF5. PTHR13847:SF5. 1 hit.
Pfam	PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
PIRSF	PIRSF006487. GcvT. 1 hit.
TIGRFAMs	TIGR00528. GcvT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCST_BACFN

Accession

Primary (citable) accession number: Q5LFT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 21, 2005
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program