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Protein

Biotin biosynthesis bifunctional protein BioAB

Gene

bioB

Organism
Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes two activities which are involved in the biotine biosynthesis: the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism, and the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA).By similarity

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.
S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate.

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.By similarity
  • pyridoxal 5'-phosphate

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin biosynthesis bifunctional protein BioAB (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Pathwayi: biotin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route).
Proteins known to be involved in this subpathway in this organism are:
  1. Biotin biosynthesis bifunctional protein BioAB (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route), the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi62Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi66Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi69Iron-sulfur 1 (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi106Iron-sulfur 2 (2Fe-2S)By similarity1
Metal bindingi138Iron-sulfur 2 (2Fe-2S)By similarity1
Metal bindingi198Iron-sulfur 2 (2Fe-2S)By similarity1
Metal bindingi268Iron-sulfur 2 (2Fe-2S)By similarity1
Sitei333Participates in the substrate recognition with KAPA and in a stacking interaction with the adenine ring of SAMBy similarity1
Binding sitei3687-keto-8-aminopelargonic acidBy similarity1
Binding sitei4617-keto-8-aminopelargonic acidBy similarity1
Binding sitei562Pyridoxal phosphateBy similarity1
Binding sitei5917-keto-8-aminopelargonic acidBy similarity1
Binding sitei6247-keto-8-aminopelargonic acid; via carbonyl oxygenBy similarity1
Binding sitei7087-keto-8-aminopelargonic acidBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, Pyridoxal phosphate, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBFRA272559:GKF0-1535-MONOMER.
UniPathwayiUPA00078; UER00160.
UPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin biosynthesis bifunctional protein BioAB
Including the following 2 domains:
Biotin synthase BioB (EC:2.8.1.6)
Adenosylmethionine-8-amino-7-oxononanoate aminotransferase BioA (EC:2.6.1.62)
Alternative name(s):
7,8-diamino-pelargonic acid aminotransferase
Short name:
DAPA AT
Short name:
DAPA aminotransferase
7,8-diaminononanoate synthase
Short name:
DANS
Diaminopelargonic acid synthase
Gene namesi
Name:bioB
Ordered Locus Names:BF1617
OrganismiBacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343)
Taxonomic identifieri272559 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
Proteomesi
  • UP000006731 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003812271 – 748Biotin biosynthesis bifunctional protein BioABAdd BLAST748

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei591N6-(pyridoxal phosphate)lysineBy similarity1

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi272559.BF1617.

Structurei

3D structure databases

ProteinModelPortaliQ5LEY1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni428 – 429Pyridoxal phosphate bindingBy similarity2
Regioni625 – 626Pyridoxal phosphate bindingBy similarity2

Sequence similaritiesi

In the N-terminal section; belongs to the radical SAM superfamily. Biotin synthase family.Curated
In the C-terminal section; belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. BioA subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105C8Y. Bacteria.
COG0161. LUCA.
COG0502. LUCA.
HOGENOMiHOG000291940.
KOiK00833.
OMAiKWCAQSS.

Family and domain databases

CDDicd00610. OAT_like. 1 hit.
Gene3Di3.20.20.70. 1 hit.
3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
HAMAPiMF_00834. BioA. 1 hit.
MF_01694. BioB. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR005814. Aminotrans_3.
IPR010722. BATS_dom.
IPR005815. BioA.
IPR002684. Biotin_synth/BioAB.
IPR006638. Elp3/MiaB/NifB.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 2 hits.
PfamiPF00202. Aminotran_3. 1 hit.
PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00508. bioA. 1 hit.
TIGR00433. bioB. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5LEY1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIEEIKNQV LQGTAISREQ AEWLALYPRK EELYDAAHDI TTACASQEFD
60 70 80 90 100
MCSIINARSG RCPENCKWCA QSSHYKTKAD VYDLVSADEC LRQAKYNEAQ
110 120 130 140 150
GVNRFSLVTS GRKPSPKNMK ELCVAARRMR RHSSIRLCAS LGLLDEEELQ
160 170 180 190 200
ALYDAGVTRY HCNLETAPSH FDSLCTTHTQ EQKLKTLHAA RRVGMDLCCG
210 220 230 240 250
GIIGMGETVE QRIEFAFTLR DLNIQSIPIN LLQPIPGTPL EHQSPLSEEE
260 270 280 290 300
ILTTVALFRF INPAAYLRFA GGRSQLTPEA VRKSLYIGIN SAIVGDLLTT
310 320 330 340 350
LGSKVSDDKE MILSEGYHFA DSQFDREHLW HPYTSTSNPL PVYKVKRADG
360 370 380 390 400
ATITLESGQT LIEGMSSWWC AVHGYNHPIL NQAVQDQLSR MSHVMFGGLT
410 420 430 440 450
HDPAIELGKL LLPLVPPSMQ KIFYADSGSV AVEVALKMAV QYWYAAGKPE
460 470 480 490 500
KNNFVTIRNG YHGDTWNAMS VCDPVTGMHS IFGSALPIRH FLPAPSSRFG
510 520 530 540 550
DEWNPEDIRP LEYLLEKHAD ELAAFILEPI VQGAGGMRFY HPEYLKEAAR
560 570 580 590 600
LCHRYGVLLI FDEIATGFGR TGKLFAWEHA GVEPDIMCIG KALTGGYMTL
610 620 630 640 650
SAVLTTNEVA DCISNHAPGA FMHGPTFMGN PLACAVACAS VRLLLTSGWQ
660 670 680 690 700
ENVKRIEAQL NRELAPAREL PQVADVRVLG AIGVIEMKEP VNMAYLQRRF
710 720 730 740
VEEGIWLRPF GKLIYVMPPF IITPEQLTKL TEGMIRIISN GLPGSQTK
Length:748
Mass (Da):83,164
Last modified:June 21, 2005 - v1
Checksum:i611586B40A861A51
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR626927 Genomic DNA. Translation: CAH07318.1.
RefSeqiWP_010992602.1. NC_003228.3.

Genome annotation databases

EnsemblBacteriaiCAH07318; CAH07318; BF9343_1537.
KEGGibfs:BF9343_1537.
PATRICi21039656. VBIBacFra29119_1576.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR626927 Genomic DNA. Translation: CAH07318.1.
RefSeqiWP_010992602.1. NC_003228.3.

3D structure databases

ProteinModelPortaliQ5LEY1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272559.BF1617.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAH07318; CAH07318; BF9343_1537.
KEGGibfs:BF9343_1537.
PATRICi21039656. VBIBacFra29119_1576.

Phylogenomic databases

eggNOGiENOG4105C8Y. Bacteria.
COG0161. LUCA.
COG0502. LUCA.
HOGENOMiHOG000291940.
KOiK00833.
OMAiKWCAQSS.

Enzyme and pathway databases

UniPathwayiUPA00078; UER00160.
UPA00078; UER00162.
BioCyciBFRA272559:GKF0-1535-MONOMER.

Family and domain databases

CDDicd00610. OAT_like. 1 hit.
Gene3Di3.20.20.70. 1 hit.
3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
HAMAPiMF_00834. BioA. 1 hit.
MF_01694. BioB. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR005814. Aminotrans_3.
IPR010722. BATS_dom.
IPR005815. BioA.
IPR002684. Biotin_synth/BioAB.
IPR006638. Elp3/MiaB/NifB.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 2 hits.
PfamiPF00202. Aminotran_3. 1 hit.
PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR00508. bioA. 1 hit.
TIGR00433. bioB. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBIOAB_BACFN
AccessioniPrimary (citable) accession number: Q5LEY1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 21, 2005
Last modified: November 2, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.