Reviewed, UniProtKB/Swiss-Prot Q5LES4 (CYSD_BACFN)

Names and origin

Protein names

Recommended name:
    Sulfate adenylyltransferase subunit 2
    EC=2.7.7.4
Alternative name(s):
    Sulfate adenylate transferase
      Short name=SAT
    ATP-sulfurylase small subunit

Gene names

Name:	cysD
Ordered Locus Names:	BF1676

Organism

Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]

Taxonomic identifier

272559 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + sulfate = diphosphate + adenylyl sulfate. HAMAP MF_00064
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 1/3. HAMAP MF_00064
Subunit structure	Heterodimer composed of cysD, the smaller subunit, and cysN By similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysD subfamily.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sulfate assimilation Inferred from electronic annotation. Source: HAMAP sulfate reduction Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW sulfate adenylyltransferase (ATP) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	Sulfate adenylyltransferase subunit 2 HAMAP MF_00064		PRO_0000340179

Sequences

	Sequence		Length	Mass (Da)
	Q5LES4-1 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: AAD7CD766089EC19	FASTA	303	35,840
	10 20 30 40 50 60 MKEEYKLSHL KELEAESIHI IREVAAEFEN PVMLYSIGKD SSVMVRLAEK AFYPGKVPFP 70 80 90 100 110 120 LMHIDSKWKF KEMIQFRDEY AKKYGWNLIV ESNMEAFHAG VGPFTHGSKV HTDLMKTQAL 130 140 150 160 170 180 LRALDKYKFD AAFGGARRDE EKSRAKERIF SFRDKFHQWD PKNQRPELWD IYNARVHKGE 190 200 210 220 230 240 SIRVFPLSNW TELDIWQYIR LENIPIVPLY YAKERPVVQM DGNLIMADDE RLPEKYRDQI 250 260 270 280 290 300 EMKMVRFRTL GCWPLTGAVE SEADTIEKIV EEMMTTTKSE RTTRVIDFDQ EGSMEQKKRE GYF

References

[1]

"Extensive DNA inversions in the B. fragilis genome control variable gene expression." Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V., Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A., Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A. , Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J., Barrell B.G., Parkhill J. Science 307:1463-1465(2005) [PubMed: 15746427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR626927 Genomic DNA. Translation: CAH07376.1.
RefSeq	YP_211314.1.
3D structure databases
SMR	Q5LES4. Positions 6-212.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5LES4.
Genome annotation databases
GeneID	3287496.
GenomeReviews	Gene locus BF1676 in contig CR626927_GR.
KEGG	bfs:BF1676.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5LES4.
OMA	SLRVFPL.
Enzyme and pathway databases
BioCyc	BFRA272559:BF1676-MON.
Family and domain databases
HAMAP	MF_00064. [Tree]
InterPro	IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. IPR011784. SO4_adenylTrfase_ssu. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
PIRSF	PIRSF002936. CysDAde_trans. 1 hit.
TIGRFAMs	TIGR02039. CysD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSD_BACFN

Accession

Primary (citable) accession number: Q5LES4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	June 21, 2005
Last modified:	November 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)