Q5LEQ6 (SYT_BACFN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Threonine--tRNA ligase
EC=6.1.1.3

Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	BF1694

Organism

Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]

Taxonomic identifier

272559 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	646 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00184
Subunit structure	Homodimer By similarity. HAMAP MF_00184
Subcellular location	Cytoplasm HAMAP MF_00184.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 646	646	Threonine--tRNA ligase HAMAP MF_00184		PRO_0000100935
Regions
	Region	242 – 541	300	Catalytic HAMAP MF_00184
Sites
	Metal binding	337	1	Zinc; catalytic By similarity
	Metal binding	388	1	Zinc; catalytic By similarity
	Metal binding	518	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5LEQ6 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: 22C3B3A70B4B73EC	FASTA	646	74,406
	10 20 30 40 50 60 MIKITFPDGS VREYNEGVNG LQIAESISSR LAQDVLACGV NGEIYDLGRP INEDASVVLY 70 80 90 100 110 120 KWEDEQGKHA FWHTSAHLLA EALQELYPGI QFGIGPAIEN GFYYDVDPGE AVIKEADLPA 130 140 150 160 170 180 IEAKMAELVA KKEAVVRRDI AKGDALKMFG DRGETYKCEL ISELEDGHIT TYTQGDFTDL 190 200 210 220 230 240 CRGPHLMTTA PIKAIKLTSV AGAYWRGHED RKMLTRIYGI TFPKKKMLDE YLALMEEAKK 250 260 270 280 290 300 RDHRKIGKEM QLFMFSDTVG KGLPMWLPKG TALRLRLQDF LRRIQTRYDY QEVITPPIGN 310 320 330 340 350 360 KLLYVTSGHY AKYGKDAFQP IHTPEEGEEY FLKPMNCPHH CEIYKNFPRS YKDLPLRIAE 370 380 390 400 410 420 FGTVCRYEQS GELHGLTRVR SFTQDDAHIF CRPDQVKGEF LRVMDIISIV FRSMDFDNFE 430 440 450 460 470 480 AQISLRDKVN REKYIGSDEN WEKAEQAIIE ACEEKGLKAK IEYGEAAFYG PKLDFMVKDA 490 500 510 520 530 540 IGRRWQLGTI QVDYNLPERF ELEYMGSDNQ KHRPVMIHRA PFGSMERFVA VLIEHTAGKF 550 560 570 580 590 600 PLWLTPEQVV ILPISEKFNE YAEKVKTYLK MKEIRAIVDD RNEKIGRKIR DNEMKRIPYM 610 620 630 640 LIVGEKEAEN GEVSVRRQGE GDKGTMKFEE FGEILNEEVQ NMINKW

References

[1]

"Extensive DNA inversions in the B. fragilis genome control variable gene expression." Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V., Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A., Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A. , Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J., Barrell B.G., Parkhill J. Science 307:1463-1465(2005) [PubMed: 15746427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25285 / NCTC 9343.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR626927 Genomic DNA. Translation: CAH07394.1.
RefSeq	YP_211332.1. NC_003228.3.
3D structure databases
ProteinModelPortal	Q5LEQ6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5LEQ6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3287499.
GenomeReviews	Gene locus BF1694 in contig CR626927_GR.
KEGG	bfs:BF1694.
PATRIC	21039811. VBIBacFra29119_1653.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0441.
HOGENOM	HBG352811.
OMA	GRKWQLG.
ProtClustDB	PRK00413.
Enzyme and pathway databases
BioCyc	BFRA272559:BF1694-MONOMER.
Family and domain databases
HAMAP	MF_00184. Thr_tRNA_synth. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR012675. Beta-grasp_ferredoxin-type. IPR004095. TGS. IPR012676. TGS-like. IPR002320. Thr-tRNA-synth_IIa. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
KO	K01868.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF81271. TGS-like. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00418. ThrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_BACFN

Accession

Primary (citable) accession number: Q5LEQ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	June 21, 2005
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program