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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Bacteroides fragilis (strain ATCC 25285 / NCTC 9343)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei692 – 6921ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBFRA272559:GKF0-2306-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:BF2367
OrganismiBacteroides fragilis (strain ATCC 25285 / NCTC 9343)
Taxonomic identifieri272559 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
ProteomesiUP000006731 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11411141Isoleucine--tRNA ligasePRO_0000098521Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272559.BF2367.

Structurei

3D structure databases

ProteinModelPortaliQ5LCU8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi50 – 6011"HIGH" regionAdd
BLAST
Motifi689 – 6935"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiIPLPIWR.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5LCU8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKKFAEYSQ FDLSKVNKDV LKKWDENQVF AKSMTEREGC PSFVFFEGPP
60 70 80 90 100
SANGMPGIHH VMARSIKDIF CRYKTMKGYQ VKRKAGWDTH GLPVELGVEK
110 120 130 140 150
SLGITKEDIG KTISVAEYNA HCRQDVMKFT KEWEDLTHKM GYWVDMKHPY
160 170 180 190 200
ITYDNRYIET LWWLLKQLYK KGLLYKGYTI QPYSPAAGTG LSSHELNQPG
210 220 230 240 250
CYRDVKDTTV VAQFKMKNPK PEMAQWGTPY FLAWTTTPWT LPSNTALCVG
260 270 280 290 300
PKIDYVAVQS YNAYTGQPIT VVLAKALLNA HFNPKAAELK LEDYKAGDKL
310 320 330 340 350
VPFKVIAEYK GPDLVGMEYE QLIPWVNPGE GAFRVILGDY VTTEDGTGIV
360 370 380 390 400
HIAPTFGADD AQVAKAAGIP PLQLVNKKGE LRPMVDLTGK FYTLDELDED
410 420 430 440 450
FIKQRVNVDL YKEYAGRFVK NAYDPNLSDQ DESLDVSICM MMKVNNQAFK
460 470 480 490 500
IEKHVHNYPH CWRTDKPVLY YPLDSWFIRS TACKERMIEL NKTINWKPES
510 520 530 540 550
TGTGRFGKWL ENLNDWNLSR SRYWGTPLPI WRTEDNSDEK CIESVEELYN
560 570 580 590 600
EIEKSVAAGY MQSNPYKDKG FVPGEYNEEN YNKIDLHRPY VDDIILVSKD
610 620 630 640 650
GKPMKREADL IDVWFDSGAM PYAQIHYPFE NKELLDSHQV YPADFIAEGV
660 670 680 690 700
DQTRGWFFTL HAIATMVFDS VSYKAVISNG LVLDKNGNKM SKRLGNAVDP
710 720 730 740 750
FSTIEQYGSD PLRWYMITNS SPWDNLKFDV DGIEEVRRKF FGTLYNTYSF
760 770 780 790 800
FALYANVDGF EYKEADLPMN ERPEIDRWIL SVLNTLVKEV DTCYNEYEPT
810 820 830 840 850
KAGRLISDFV NDNLSNWYVR LNRKRFWGGG FTQDKLSAYQ TLYTCLETVA
860 870 880 890 900
KLMAPIAPFY ADRLYSDLIG VTGRDNVVSV HLAKFPEYNE KMVDKELEAQ
910 920 930 940 950
MQMAQDVTSM VLALRRKVNI KVRQPLQCIM IPVVDEVQKA HIEAVKALIM
960 970 980 990 1000
SEVNVKEIKF VDGAAGVLVK KVKCDFKKLG PKFGKQMKAV AAAVAEMSQE
1010 1020 1030 1040 1050
AIAELEKNGK YTFDLGGAEA VIESADVEIF SEDIPGWLVA NEGKLTVALE
1060 1070 1080 1090 1100
VTVTDELRRE GIARELVNRI QNIRKSSGFE ITDKIKLTLS KNPQTDDAVN
1110 1120 1130 1140
EYNSYICNQV LGTSLTLADE VKDGTELNFD DFSLFVNVVK E
Length:1,141
Mass (Da):130,111
Last modified:June 20, 2005 - v1
Checksum:iC01F15AE981C9BB5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR626927 Genomic DNA. Translation: CAH08065.1.
RefSeqiYP_211991.1. NC_003228.3.

Genome annotation databases

EnsemblBacteriaiCAH08065; CAH08065; BF9343_2284.
KEGGibfs:BF9343_2284.
PATRICi21041256. VBIBacFra29119_2369.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR626927 Genomic DNA. Translation: CAH08065.1.
RefSeqiYP_211991.1. NC_003228.3.

3D structure databases

ProteinModelPortaliQ5LCU8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272559.BF2367.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAH08065; CAH08065; BF9343_2284.
KEGGibfs:BF9343_2284.
PATRICi21041256. VBIBacFra29119_2369.

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiIPLPIWR.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciBFRA272559:GKF0-2306-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25285 / NCTC 9343.

Entry informationi

Entry nameiSYI_BACFN
AccessioniPrimary (citable) accession number: Q5LCU8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 19, 2005
Last sequence update: June 20, 2005
Last modified: March 31, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.