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Q5LCU8

- SYI_BACFN

UniProt

Q5LCU8 - SYI_BACFN

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Bacteroides fragilis (strain ATCC 25285 / NCTC 9343)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (21 Jun 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei692 – 6921ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciBFRA272559:GKF0-2306-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:BF2367
    OrganismiBacteroides fragilis (strain ATCC 25285 / NCTC 9343)
    Taxonomic identifieri272559 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
    ProteomesiUP000006731: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11411141Isoleucine--tRNA ligasePRO_0000098521Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi272559.BF2367.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5LCU8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi50 – 6011"HIGH" regionAdd
    BLAST
    Motifi689 – 6935"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q5LCU8-1 [UniParc]FASTAAdd to Basket

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    MSKKFAEYSQ FDLSKVNKDV LKKWDENQVF AKSMTEREGC PSFVFFEGPP     50
    SANGMPGIHH VMARSIKDIF CRYKTMKGYQ VKRKAGWDTH GLPVELGVEK 100
    SLGITKEDIG KTISVAEYNA HCRQDVMKFT KEWEDLTHKM GYWVDMKHPY 150
    ITYDNRYIET LWWLLKQLYK KGLLYKGYTI QPYSPAAGTG LSSHELNQPG 200
    CYRDVKDTTV VAQFKMKNPK PEMAQWGTPY FLAWTTTPWT LPSNTALCVG 250
    PKIDYVAVQS YNAYTGQPIT VVLAKALLNA HFNPKAAELK LEDYKAGDKL 300
    VPFKVIAEYK GPDLVGMEYE QLIPWVNPGE GAFRVILGDY VTTEDGTGIV 350
    HIAPTFGADD AQVAKAAGIP PLQLVNKKGE LRPMVDLTGK FYTLDELDED 400
    FIKQRVNVDL YKEYAGRFVK NAYDPNLSDQ DESLDVSICM MMKVNNQAFK 450
    IEKHVHNYPH CWRTDKPVLY YPLDSWFIRS TACKERMIEL NKTINWKPES 500
    TGTGRFGKWL ENLNDWNLSR SRYWGTPLPI WRTEDNSDEK CIESVEELYN 550
    EIEKSVAAGY MQSNPYKDKG FVPGEYNEEN YNKIDLHRPY VDDIILVSKD 600
    GKPMKREADL IDVWFDSGAM PYAQIHYPFE NKELLDSHQV YPADFIAEGV 650
    DQTRGWFFTL HAIATMVFDS VSYKAVISNG LVLDKNGNKM SKRLGNAVDP 700
    FSTIEQYGSD PLRWYMITNS SPWDNLKFDV DGIEEVRRKF FGTLYNTYSF 750
    FALYANVDGF EYKEADLPMN ERPEIDRWIL SVLNTLVKEV DTCYNEYEPT 800
    KAGRLISDFV NDNLSNWYVR LNRKRFWGGG FTQDKLSAYQ TLYTCLETVA 850
    KLMAPIAPFY ADRLYSDLIG VTGRDNVVSV HLAKFPEYNE KMVDKELEAQ 900
    MQMAQDVTSM VLALRRKVNI KVRQPLQCIM IPVVDEVQKA HIEAVKALIM 950
    SEVNVKEIKF VDGAAGVLVK KVKCDFKKLG PKFGKQMKAV AAAVAEMSQE 1000
    AIAELEKNGK YTFDLGGAEA VIESADVEIF SEDIPGWLVA NEGKLTVALE 1050
    VTVTDELRRE GIARELVNRI QNIRKSSGFE ITDKIKLTLS KNPQTDDAVN 1100
    EYNSYICNQV LGTSLTLADE VKDGTELNFD DFSLFVNVVK E 1141
    Length:1,141
    Mass (Da):130,111
    Last modified:June 21, 2005 - v1
    Checksum:iC01F15AE981C9BB5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR626927 Genomic DNA. Translation: CAH08065.1.
    RefSeqiYP_211991.1. NC_003228.3.

    Genome annotation databases

    EnsemblBacteriaiCAH08065; CAH08065; BF9343_2284.
    GeneIDi3289145.
    KEGGibfs:BF2367.
    PATRICi21041256. VBIBacFra29119_2369.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR626927 Genomic DNA. Translation: CAH08065.1 .
    RefSeqi YP_211991.1. NC_003228.3.

    3D structure databases

    ProteinModelPortali Q5LCU8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272559.BF2367.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAH08065 ; CAH08065 ; BF9343_2284 .
    GeneIDi 3289145.
    KEGGi bfs:BF2367.
    PATRICi 21041256. VBIBacFra29119_2369.

    Phylogenomic databases

    eggNOGi COG0060.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci BFRA272559:GKF0-2306-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 25285 / NCTC 9343.

    Entry informationi

    Entry nameiSYI_BACFN
    AccessioniPrimary (citable) accession number: Q5LCU8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: June 21, 2005
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3