Reviewed, UniProtKB/Swiss-Prot Q5LCU8 (SYI_BACFN)

Names and origin

Protein names

Recommended name:
    Isoleucyl-tRNA synthetase
    EC=6.1.1.5
Alternative name(s):
    Isoleucine--tRNA ligase
      Short name=IleRS

Gene names

Name:	ileS
Ordered Locus Names:	BF2367

Organism

Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]

Taxonomic identifier

272559 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	1141 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.
Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP MF_02003
Cofactor	Zinc By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1141	1141	Isoleucyl-tRNA synthetase HAMAP MF_02003		PRO_0000098521
Regions
	Motif	50 – 60	11	"HIGH" region HAMAP MF_02003
	Motif	689 – 693	5	"KMSKS" region HAMAP MF_02003
Sites
	Binding site	692	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5LCU8-1 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: C01F15AE981C9BB5	FASTA	1,141	130,111
	10 20 30 40 50 60 MSKKFAEYSQ FDLSKVNKDV LKKWDENQVF AKSMTEREGC PSFVFFEGPP SANGMPGIHH 70 80 90 100 110 120 VMARSIKDIF CRYKTMKGYQ VKRKAGWDTH GLPVELGVEK SLGITKEDIG KTISVAEYNA 130 140 150 160 170 180 HCRQDVMKFT KEWEDLTHKM GYWVDMKHPY ITYDNRYIET LWWLLKQLYK KGLLYKGYTI 190 200 210 220 230 240 QPYSPAAGTG LSSHELNQPG CYRDVKDTTV VAQFKMKNPK PEMAQWGTPY FLAWTTTPWT 250 260 270 280 290 300 LPSNTALCVG PKIDYVAVQS YNAYTGQPIT VVLAKALLNA HFNPKAAELK LEDYKAGDKL 310 320 330 340 350 360 VPFKVIAEYK GPDLVGMEYE QLIPWVNPGE GAFRVILGDY VTTEDGTGIV HIAPTFGADD 370 380 390 400 410 420 AQVAKAAGIP PLQLVNKKGE LRPMVDLTGK FYTLDELDED FIKQRVNVDL YKEYAGRFVK 430 440 450 460 470 480 NAYDPNLSDQ DESLDVSICM MMKVNNQAFK IEKHVHNYPH CWRTDKPVLY YPLDSWFIRS 490 500 510 520 530 540 TACKERMIEL NKTINWKPES TGTGRFGKWL ENLNDWNLSR SRYWGTPLPI WRTEDNSDEK 550 560 570 580 590 600 CIESVEELYN EIEKSVAAGY MQSNPYKDKG FVPGEYNEEN YNKIDLHRPY VDDIILVSKD 610 620 630 640 650 660 GKPMKREADL IDVWFDSGAM PYAQIHYPFE NKELLDSHQV YPADFIAEGV DQTRGWFFTL 670 680 690 700 710 720 HAIATMVFDS VSYKAVISNG LVLDKNGNKM SKRLGNAVDP FSTIEQYGSD PLRWYMITNS 730 740 750 760 770 780 SPWDNLKFDV DGIEEVRRKF FGTLYNTYSF FALYANVDGF EYKEADLPMN ERPEIDRWIL 790 800 810 820 830 840 SVLNTLVKEV DTCYNEYEPT KAGRLISDFV NDNLSNWYVR LNRKRFWGGG FTQDKLSAYQ 850 860 870 880 890 900 TLYTCLETVA KLMAPIAPFY ADRLYSDLIG VTGRDNVVSV HLAKFPEYNE KMVDKELEAQ 910 920 930 940 950 960 MQMAQDVTSM VLALRRKVNI KVRQPLQCIM IPVVDEVQKA HIEAVKALIM SEVNVKEIKF 970 980 990 1000 1010 1020 VDGAAGVLVK KVKCDFKKLG PKFGKQMKAV AAAVAEMSQE AIAELEKNGK YTFDLGGAEA 1030 1040 1050 1060 1070 1080 VIESADVEIF SEDIPGWLVA NEGKLTVALE VTVTDELRRE GIARELVNRI QNIRKSSGFE 1090 1100 1110 1120 1130 1140 ITDKIKLTLS KNPQTDDAVN EYNSYICNQV LGTSLTLADE VKDGTELNFD DFSLFVNVVK E

References

[1]

"Extensive DNA inversions in the B. fragilis genome control variable gene expression." Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V., Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A., Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A. , Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J., Barrell B.G., Parkhill J. Science 307:1463-1465(2005) [PubMed: 15746427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR626927 Genomic DNA. Translation: CAH08065.1.
RefSeq	YP_211991.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5LCU8.
Genome annotation databases
GeneID	3289145.
GenomeReviews	Gene locus BF2367 in contig CR626927_GR.
KEGG	bfs:BF2367.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5LCU8.
OMA	SNWYVRR.
Enzyme and pathway databases
BioCyc	BFRA272559:BF2367-MON.
Family and domain databases
HAMAP	MF_02003. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-synt_Ia. IPR015905. Ile-tRNA-synt_Ia_N. IPR018353. Isoleucyl-tRNA_synthetase. IPR014729. Rossmann-like_a/b/a_fold. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11946:SF9. Ile-tRNA-synt_Ia. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI_BACFN

Accession

Primary (citable) accession number: Q5LCU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	June 21, 2005
Last modified:	November 3, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)