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Q5LCU8

- SYI_BACFN

UniProt

Q5LCU8 - SYI_BACFN

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Protein
Isoleucine--tRNA ligase
Gene
ileS, BF2367
Organism
Bacteroides fragilis (strain ATCC 25285 / NCTC 9343)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei692 – 6921ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBFRA272559:GKF0-2306-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:BF2367
OrganismiBacteroides fragilis (strain ATCC 25285 / NCTC 9343)
Taxonomic identifieri272559 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
ProteomesiUP000006731: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11411141Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098521Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi272559.BF2367.

Structurei

3D structure databases

ProteinModelPortaliQ5LCU8.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi50 – 6011"HIGH" regionUniRule annotation
Add
BLAST
Motifi689 – 6935"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q5LCU8-1 [UniParc]FASTAAdd to Basket

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MSKKFAEYSQ FDLSKVNKDV LKKWDENQVF AKSMTEREGC PSFVFFEGPP     50
SANGMPGIHH VMARSIKDIF CRYKTMKGYQ VKRKAGWDTH GLPVELGVEK 100
SLGITKEDIG KTISVAEYNA HCRQDVMKFT KEWEDLTHKM GYWVDMKHPY 150
ITYDNRYIET LWWLLKQLYK KGLLYKGYTI QPYSPAAGTG LSSHELNQPG 200
CYRDVKDTTV VAQFKMKNPK PEMAQWGTPY FLAWTTTPWT LPSNTALCVG 250
PKIDYVAVQS YNAYTGQPIT VVLAKALLNA HFNPKAAELK LEDYKAGDKL 300
VPFKVIAEYK GPDLVGMEYE QLIPWVNPGE GAFRVILGDY VTTEDGTGIV 350
HIAPTFGADD AQVAKAAGIP PLQLVNKKGE LRPMVDLTGK FYTLDELDED 400
FIKQRVNVDL YKEYAGRFVK NAYDPNLSDQ DESLDVSICM MMKVNNQAFK 450
IEKHVHNYPH CWRTDKPVLY YPLDSWFIRS TACKERMIEL NKTINWKPES 500
TGTGRFGKWL ENLNDWNLSR SRYWGTPLPI WRTEDNSDEK CIESVEELYN 550
EIEKSVAAGY MQSNPYKDKG FVPGEYNEEN YNKIDLHRPY VDDIILVSKD 600
GKPMKREADL IDVWFDSGAM PYAQIHYPFE NKELLDSHQV YPADFIAEGV 650
DQTRGWFFTL HAIATMVFDS VSYKAVISNG LVLDKNGNKM SKRLGNAVDP 700
FSTIEQYGSD PLRWYMITNS SPWDNLKFDV DGIEEVRRKF FGTLYNTYSF 750
FALYANVDGF EYKEADLPMN ERPEIDRWIL SVLNTLVKEV DTCYNEYEPT 800
KAGRLISDFV NDNLSNWYVR LNRKRFWGGG FTQDKLSAYQ TLYTCLETVA 850
KLMAPIAPFY ADRLYSDLIG VTGRDNVVSV HLAKFPEYNE KMVDKELEAQ 900
MQMAQDVTSM VLALRRKVNI KVRQPLQCIM IPVVDEVQKA HIEAVKALIM 950
SEVNVKEIKF VDGAAGVLVK KVKCDFKKLG PKFGKQMKAV AAAVAEMSQE 1000
AIAELEKNGK YTFDLGGAEA VIESADVEIF SEDIPGWLVA NEGKLTVALE 1050
VTVTDELRRE GIARELVNRI QNIRKSSGFE ITDKIKLTLS KNPQTDDAVN 1100
EYNSYICNQV LGTSLTLADE VKDGTELNFD DFSLFVNVVK E 1141
Length:1,141
Mass (Da):130,111
Last modified:June 21, 2005 - v1
Checksum:iC01F15AE981C9BB5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR626927 Genomic DNA. Translation: CAH08065.1.
RefSeqiYP_211991.1. NC_003228.3.

Genome annotation databases

EnsemblBacteriaiCAH08065; CAH08065; BF9343_2284.
GeneIDi3289145.
KEGGibfs:BF2367.
PATRICi21041256. VBIBacFra29119_2369.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR626927 Genomic DNA. Translation: CAH08065.1 .
RefSeqi YP_211991.1. NC_003228.3.

3D structure databases

ProteinModelPortali Q5LCU8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272559.BF2367.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAH08065 ; CAH08065 ; BF9343_2284 .
GeneIDi 3289145.
KEGGi bfs:BF2367.
PATRICi 21041256. VBIBacFra29119_2369.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BFRA272559:GKF0-2306-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25285 / NCTC 9343.

Entry informationi

Entry nameiSYI_BACFN
AccessioniPrimary (citable) accession number: Q5LCU8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: June 21, 2005
Last modified: May 14, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi