Q5LCU6 (Q5LCU6_BACFN) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Lipoprotein signal peptidase HAMAP MF_00161
EC=3.4.23.36 HAMAP MF_00161

Alternative name(s):
Prolipoprotein signal peptidase HAMAP MF_00161
Signal peptidase II HAMAP MF_00161

Gene names

Name:	lspA HAMAP MF_00161
Ordered Locus Names:	BF2369
ORF Names:	BF9343_2286 EMBL CAH08067.1

Organism

Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP] EMBL CAH08067.1

Taxonomic identifier

272559 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins By similarity. HAMAP MF_00161 SAAS SAAS001872
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP MF_00161 SAAS SAAS001872
Pathway	Protein modification; lipoprotein biosynthesis (signal peptide cleavage). HAMAP MF_00161 SAAS SAAS001872
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_00161.
Sequence similarities	Belongs to the peptidase A8 family. HAMAP MF_00161

Ontologies

Keywords
Cellular component	Cell inner membrane HAMAP MF_00161 Cell membrane Membrane
Domain	Transmembrane Transmembrane helix HAMAP MF_00161 SAAS SAAS001872
Molecular function	Aspartyl protease HAMAP MF_00161 SAAS SAAS001872 Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Transmembrane	9 – 29	21	Helical; By similarity HAMAP MF_00161
	Transmembrane	61 – 81	21	Helical; By similarity HAMAP MF_00161
	Transmembrane	99 – 119	21	Helical; By similarity HAMAP MF_00161
	Transmembrane	170 – 190	21	Helical; By similarity HAMAP MF_00161
Sites
	Active site	111	1	By similarity HAMAP MF_00161
	Active site	181	1	By similarity HAMAP MF_00161

Sequences

	Sequence		Length	Mass (Da)
	Q5LCU6 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: 3F837FA4995AC324	FASTA	210	24,198
	10 20 30 40 50 60 MKKLLTKGQI AILVIFSVLI IDQVIKIWIK THMYWHESIR ITDWFYIYFT ENNGMAFGME 70 80 90 100 110 120 LFGKLFLTTF RIVAVGLIGW YLYKIVKRGL KTGYIICVSL ILTGALGNII DSVFYGVIFN 130 140 150 160 170 180 ESTHSQIASF MPDGGGYSTW FYGKVVDMFY FPIIDTNWPT WMPFVGGEHF IFFSPIFNFA 190 200 210 DAAISCGIIA LLLFYSKYLN DSYHHSVTKK

References

[1]

"Extensive DNA inversions in the B. fragilis genome control variable gene expression." Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V., Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A., Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A. , Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J., Barrell B.G., Parkhill J. Science 307:1463-1465(2005) [PubMed: 15746427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR626927 Genomic DNA. Translation: CAH08067.1.
RefSeq	YP_211993.1. NC_003228.3.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5LCU6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3285185.
GenomeReviews	Gene locus BF2369 in contig CR626927_GR.
KEGG	bfs:BF2369.
PATRIC	21041260. VBIBacFra29119_2371.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	NOG78647.
HOGENOM	HBG724422.
OMA	HGKVVDM.
ProtClustDB	PRK14788.
Family and domain databases
HAMAP	MF_00161. LspA. [Tree]
InterPro	IPR001872. Peptidase_A8. [Graphical view]
KO	K03101.
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
ProtoNet	Search...

Entry information

Entry name

Q5LCU6_BACFN

Accession

Primary (citable) accession number: Q5LCU6

Entry history

Integrated into UniProtKB/TrEMBL:	June 21, 2005
Last sequence update:	June 21, 2005
Last modified:	December 14, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)