Q5LCK2 (SYD_BACFN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	BF2463

Organism

Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]

Taxonomic identifier

272559 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	585 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP MF_00044_B
Subcellular location	Cytoplasm HAMAP MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 585	585	Aspartate--tRNA ligase HAMAP MF_00044_B		PRO_0000235508

Sequences

	Sequence		Length	Mass (Da)
	Q5LCK2 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: EB1A857BEED301C2	FASTA	585	66,229
	10 20 30 40 50 60 MFRTHTCGEL RISDVNKQVK LSGWVQRSRK MGGMTFVDLR DRYGITQLVF NEEIDAELCE 70 80 90 100 110 120 RANKLGREFV IQIVGTVNER FSKNSHIPTG DIEIIVSELN ILNSAITPPF TIEDNTDGGD 130 140 150 160 170 180 DIRMKYRYLD LRRSAVRSNL ELRHKMTIEV RSYLDKLGFL EVETPVLIGS TPEGARDFVV 190 200 210 220 230 240 PSRMNPGQFY ALPQSPQTLK QLLMVSGFDR YFQIAKCFRD EDLRADRQPE FTQIDCEMSF 250 260 270 280 290 300 VEQEDVITTF EGMAKHLFKV IRNIELTEPF PRMPWSEAMR LYGSDKPDIR FGMQFVELMD 310 320 330 340 350 360 ILKGHGFSVF DNATYIGGIC AEGAAGYTRK QLDALTEFVK KPQIGAKGMV YARIEADGTV 370 380 390 400 410 420 KSSVDKFYTQ EVLQQLKEAF GAKPGDLILI LSGDDAMKTR KQLCELRLEM GNQLGLRDKN 430 440 450 460 470 480 TFACLWVVDF PLFEWSEEEG RLMAMHHPFT SPKPEDIHLL DTNPAAVRAN AYDMVINGVE 490 500 510 520 530 540 VGGGSIRIHD SQLQNKMFEL LGFTPERAQE QFGFLMNAFK FGAPPHGGLA YGLDRWVSLF 550 560 570 580 AGLDSIRDCI AFPKNNSGRD VMLDAPAALD PSQLEELNLI VDIKE

References

[1]

"Extensive DNA inversions in the B. fragilis genome control variable gene expression." Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V., Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A., Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A. , Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J., Barrell B.G., Parkhill J. Science 307:1463-1465(2005) [PubMed: 15746427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25285 / NCTC 9343.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR626927 Genomic DNA. Translation: CAH08163.1.
RefSeq	YP_212087.1. NC_003228.3.
3D structure databases
ProteinModelPortal	Q5LCK2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5LCK2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3289135.
GenomeReviews	Gene locus BF2463 in contig CR626927_GR.
KEGG	bfs:BF2463.
PATRIC	21041462. VBIBacFra29119_2468.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0173.
HOGENOM	HBG396032.
OMA	AFPKTQQ.
ProtClustDB	PRK00476.
Enzyme and pathway databases
BioCyc	BFRA272559:BF2463-MONOMER.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:3.30.1360.30. GAD_dom. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01876.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. AspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_BACFN

Accession

Primary (citable) accession number: Q5LCK2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	June 21, 2005
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program