Q5LCE6 (COBT_BACFN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase
Short name=NN:DBI PRT
EC=2.4.2.21

Alternative name(s):
N(1)-alpha-phosphoribosyltransferase

Gene names

Name:	cobT
Ordered Locus Names:	BF2519

Organism

Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]

Taxonomic identifier

272559 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the synthesis of alpha-ribazole-5'-phosphate from nicotinate mononucleotide (NAMN) and 5,6-dimethylbenzimidazole (DMB). HAMAP MF_00230
Catalytic activity	Beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole = nicotinate + alpha-ribazole 5'-phosphate. HAMAP MF_00230
Pathway	Nucleoside biosynthesis; alpha-ribazole biosynthesis; alpha-ribazole from 5,6-dimethylbenzimidazole: step 1/2. HAMAP MF_00230
Sequence similarities	Belongs to the CobT family.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cobalamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW nucleoside biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase HAMAP MF_00230		PRO_1000021575
Sites
	Active site	312	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5LCE6 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: EAD26FCFB6B4EE23	FASTA	345	37,874
	10 20 30 40 50 60 MKTFQITRPD ETIREALTDK INNLTKPKGS LGTLEELALQ IGLIQQTLTP ELRHPQNIIF 70 80 90 100 110 120 AADHGIVDEG VSLSPKEITW QQISNFLHGG AGVNFLCRQH GFELKIVDAG VDYDLPYEKG 130 140 150 160 170 180 IINMKVRKSS RNYLYEAAMT EEEMNLCIER GAEVVRQCHA EGCNVLSLGE MGIGNTSSSS 190 200 210 220 230 240 MWMTCFTHIP LELCVGAGSG LDNAGVRHKY NVLQQALDHY QGDGSAHDLI RYFGGLEMVM 250 260 270 280 290 300 AIGAMLQAAE LKMIILVDGF IMTNCILAAS QLYPEVLHYA IFGHQGDEAG HKLVLDAMGA 310 320 330 340 KPLLNLGLRL GEGTGAICSY PIIDSAVRMI NEMDNFAHAA ITKYF

References

[1]

"Extensive DNA inversions in the B. fragilis genome control variable gene expression." Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V., Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A., Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A. , Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J., Barrell B.G., Parkhill J. Science 307:1463-1465(2005) [PubMed: 15746427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25285 / NCTC 9343.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR626927 Genomic DNA. Translation: CAH08219.1.
RefSeq	YP_212143.1. NC_003228.3.
3D structure databases
HSSP	HSSP built from PDB template 1WX1 based on UniProtKB Q7SIC7.
ProteinModelPortal	Q5LCE6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5LCE6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3287468.
GenomeReviews	Gene locus BF2519 in contig CR626927_GR.
KEGG	bfs:BF2519.
PATRIC	21041586. VBIBacFra29119_2526.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2038.
HOGENOM	HBG634399.
OMA	FICSVAA.
ProtClustDB	CLSK826801.
Enzyme and pathway databases
BioCyc	BFRA272559:BF2519-MONOMER.
Family and domain databases
HAMAP	MF_00230. CobT. [Tree]
InterPro	IPR003200. Nict_dMeBzImd_PRibTrfase-like. IPR023195. Nict_dMeBzImd_PRibTrfase_N. IPR017846. Nict_dMeBzImd_PRibTrfase_pro. [Graphical view]
Gene3D	G3DSA:1.10.1610.10. Nict_dMeBzImd_PRibTrfase_N. 1 hit.
KO	K00768.
Pfam	PF02277. DBI_PRT. 1 hit. [Graphical view]
SUPFAM	SSF52733. NN:DBI_PRT. 1 hit.
TIGRFAMs	TIGR03160. CobT_DBIPRT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

COBT_BACFN

Accession

Primary (citable) accession number: Q5LCE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 21, 2005
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program