Reviewed, UniProtKB/Swiss-Prot Q5LC56 (ISPE_BACFN)

Names and origin

Protein names

Recommended name:
    4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
      Short name=CMK
    EC=2.7.1.148
Alternative name(s):
    4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	BF2610

Organism

Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]

Taxonomic identifier

272559 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity.
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphorylation Inferred from electronic annotation. Source: InterPro terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000235067
Regions
	Nucleotide binding	94 – 104	11	ATP Potential
Sites
	Active site	8	1	By similarity
	Active site	136	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5LC56-1 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: 7882D68349999D60	FASTA	274	30,787
	10 20 30 40 50 60 MITFPNAKIN LGLNITEKRP DGYHNLETVF YPIPLEDALE ITILNDSKQK FVLHQSGLEI 70 80 90 100 110 120 SGEPETNLVV KAYLLLEQEF QLPPVDIYLY KHIPSGAGLG GGSADAAFML KLLNEKFNLH 130 140 150 160 170 180 LADEKLEEYA AILGADCAFF IKNKPTFAEG IGNIFSPVDL SLKGYQLVLV KPDVFVSTRD 190 200 210 220 230 240 AFSQIKPHYP DHSLKEIIRR PVSEWKNCMF NDFEKSVFPQ YPVIEEIKKE LYSKGAIYAA 250 260 270 MSGSGSSVFG LFSPEEKITK MDFEAAFCFQ TELK

References

[1]

"Extensive DNA inversions in the B. fragilis genome control variable gene expression." Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V., Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A., Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A. , Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J., Barrell B.G., Parkhill J. Science 307:1463-1465(2005) [PubMed: 15746427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR626927 Genomic DNA. Translation: CAH08310.1.
RefSeq	YP_212233.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5LC56.
Genome annotation databases
GeneID	3287335.
GenomeReviews	Gene locus BF2610 in contig CR626927_GR.
KEGG	bfs:BF2610.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5LC56.
OMA	VFYPIPL.
Enzyme and pathway databases
BioCyc	BFRA272559:BF2610-MON.
Family and domain databases
HAMAP	MF_00061. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
TIGRFAMs	TIGR00154. ispE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_BACFN

Accession

Primary (citable) accession number: Q5LC56

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	June 21, 2005
Last modified:	November 3, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)