Reviewed, UniProtKB/Swiss-Prot Q5LAZ8 (HISX_BACFN)

Names and origin

Protein names

Recommended name:
    Histidinol dehydrogenase
      Short name=HDH
    EC=1.1.1.23

Gene names

Name:	hisD
Ordered Locus Names:	BF3029

Organism

Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]

Taxonomic identifier

272559 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine By similarity. HAMAP MF_01024
Catalytic activity	L-histidinol + H2O + 2 NAD+ = L-histidine + 2 NADH.
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_01024
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 9/9. HAMAP MF_01024
Sequence similarities	Belongs to the histidinol dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro histidinol dehydrogenase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	Histidinol dehydrogenase HAMAP MF_01024		PRO_0000135731
Sites
	Active site	322	1	Proton acceptor By similarity
	Active site	323	1	Proton acceptor By similarity
	Metal binding	255	1	Zinc By similarity
	Metal binding	258	1	Zinc By similarity
	Metal binding	356	1	Zinc By similarity
	Metal binding	415	1	Zinc By similarity
	Binding site	124	1	NAD By similarity
	Binding site	186	1	NAD By similarity
	Binding site	209	1	NAD By similarity
	Binding site	233	1	Substrate By similarity
	Binding site	255	1	Substrate By similarity
	Binding site	258	1	Substrate By similarity
	Binding site	323	1	Substrate By similarity
	Binding site	356	1	Substrate By similarity
	Binding site	410	1	Substrate By similarity
	Binding site	415	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5LAZ8-1 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: 731DC2A51FA3930E	FASTA	428	46,535
	10 20 30 40 50 60 MKLIKYPDRS QWNEILKRPV LETENLFDTV RNIINRVRAG GDRVVMEYEA VFDKAELTSL 70 80 90 100 110 120 AVTSAEIEEA EKEVPIELKA AIYLAKRNIE TFHSAQRFEG KKVDTMEGVT CWQKAVAIEK 130 140 150 160 170 180 VGLYIPGGTA PLFSTVLMLA IPAKIAGCKE IVLCTPPDKN GKVHPAILFA ARLAGVSKIF 190 200 210 220 230 240 KAGGVQAIAA MAYGTESIPK VYKIFGPGNQ YVTAAKQLVS LRDVAIDMPA GPSEVEVLAD 250 260 270 280 290 300 ESANPVFVAA DLLSQAEHGV DSQAMLVTTS EKLQTEVVYE VERQLGYLTR RDIAEKSLAS 310 320 330 340 350 360 SKLILVKDME EALELTNAYA PEHLIIETKD YMEVAGQIVN AGSVFLGAFS PESAGDYASG 370 380 390 400 410 420 TNHTLPTNGY AKAYSGVSLD SFIRKITFQE ILPSGMSAIG PAIEVMAANE HLDAHKNAVT VRLEEIRK

References

[1]

"Extensive DNA inversions in the B. fragilis genome control variable gene expression." Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V., Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A., Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A. , Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J., Barrell B.G., Parkhill J. Science 307:1463-1465(2005) [PubMed: 15746427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR626927 Genomic DNA. Translation: CAH08724.1.
RefSeq	YP_212643.1.
3D structure databases
SMR	Q5LAZ8. Positions 8-428.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5LAZ8.
Genome annotation databases
GeneID	3289178.
GenomeReviews	Gene locus BF3029 in contig CR626927_GR.
KEGG	bfs:BF3029.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0141.
HOGENOM	HBG329596.
OMA	LDAHKNA.
Enzyme and pathway databases
BioCyc	BFRA272559:BF3029-MONOMER.
Family and domain databases
HAMAP	MF_01024. HisD. [Tree]
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR001692. Histidinol_DH_CS. IPR012131. Hstdl_DH_prok-type. [Graphical view]
PANTHER	PTHR21256:SF2. Hstdl_DH_prok. 1 hit.
Pfam	PF00815. Histidinol_dh. 1 hit. [Graphical view]
PRINTS	PR00083. HOLDHDRGNASE.
TIGRFAMs	TIGR00069. hisD. 1 hit.
PROSITE	PS00611. HISOL_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HISX_BACFN

Accession

Primary (citable) accession number: Q5LAZ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	June 21, 2005
Last modified:	February 9, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)