SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q5LAS7

- RUVC_BACFN

UniProt

Q5LAS7 - RUVC_BACFN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Crossover junction endodeoxyribonuclease RuvC
Gene
ruvC, BF3101
Organism
Bacteroides fragilis (strain ATCC 25285 / NCTC 9343)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi14 – 141Magnesium By similarity
Metal bindingi74 – 741Magnesium By similarity
Metal bindingi149 – 1491Magnesium By similarity
Metal bindingi152 – 1521Magnesium By similarity

GO - Molecular functioni

  1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. nucleic acid binding Source: InterPro

GO - Biological processi

  1. DNA recombination Source: UniProtKB-HAMAP
  2. DNA repair Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBFRA272559:GKF0-3058-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvC (EC:3.1.22.4)
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene namesi
Name:ruvC
Ordered Locus Names:BF3101
OrganismiBacteroides fragilis (strain ATCC 25285 / NCTC 9343)
Taxonomic identifieri272559 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
ProteomesiUP000006731: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 188188Crossover junction endodeoxyribonuclease RuvCUniRule annotation
PRO_0000225120Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272559.BF3101.

Structurei

3D structure databases

ProteinModelPortaliQ5LAS7.

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.

Phylogenomic databases

eggNOGiCOG0817.
KOiK01159.
OMAiHRERENN.
OrthoDBiEOG6RG044.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiPS01321. RUVC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5LAS7-1 [UniParc]FASTAAdd to Basket

« Hide

MIQPVKEKII LGIDPGTTIM GYGVLRVCGT RPEMIAMGII DLRKFGNHYL    50
KLRHIHERVL SIIESYLPDE LAIEAPFFGK NVQSMLKLGR AQGVAMAAAL 100
SRDIPITEYA PLKIKMAITG NGQASKEQVA DMLQRMLHFA KEDMPVFMDA 150
TDGLAAAYCH FLQMGRPVME KGYSGWKDFI AKNPERVK 188
Length:188
Mass (Da):20,995
Last modified:June 21, 2005 - v1
Checksum:i38C131771C78B639
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR626927 Genomic DNA. Translation: CAH08796.1.
RefSeqiYP_212714.1. NC_003228.3.

Genome annotation databases

EnsemblBacteriaiCAH08796; CAH08796; BF9343_3015.
GeneIDi3288290.
KEGGibfs:BF3101.
PATRICi21042834. VBIBacFra29119_3136.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR626927 Genomic DNA. Translation: CAH08796.1 .
RefSeqi YP_212714.1. NC_003228.3.

3D structure databases

ProteinModelPortali Q5LAS7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272559.BF3101.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAH08796 ; CAH08796 ; BF9343_3015 .
GeneIDi 3288290.
KEGGi bfs:BF3101.
PATRICi 21042834. VBIBacFra29119_3136.

Phylogenomic databases

eggNOGi COG0817.
KOi K01159.
OMAi HRERENN.
OrthoDBi EOG6RG044.

Enzyme and pathway databases

BioCyci BFRA272559:GKF0-3058-MONOMER.

Family and domain databases

Gene3Di 3.30.420.10. 1 hit.
HAMAPi MF_00034. RuvC.
InterProi IPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view ]
Pfami PF02075. RuvC. 1 hit.
[Graphical view ]
PRINTSi PR00696. RSOLVASERUVC.
SUPFAMi SSF53098. SSF53098. 1 hit.
TIGRFAMsi TIGR00228. ruvC. 1 hit.
PROSITEi PS01321. RUVC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25285 / NCTC 9343.

Entry informationi

Entry nameiRUVC_BACFN
AccessioniPrimary (citable) accession number: Q5LAS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: June 21, 2005
Last modified: May 14, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi