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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi14 – 141MagnesiumUniRule annotation
Metal bindingi74 – 741MagnesiumUniRule annotation
Metal bindingi149 – 1491MagnesiumUniRule annotation
Metal bindingi152 – 1521MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBFRA272559:GKF0-3058-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
Alternative name(s):
Holliday junction nuclease RuvCUniRule annotation
Holliday junction resolvase RuvCUniRule annotation
Gene namesi
Name:ruvCUniRule annotation
Ordered Locus Names:BF3101
OrganismiBacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343)
Taxonomic identifieri272559 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
ProteomesiUP000006731 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 188188Crossover junction endodeoxyribonuclease RuvCPRO_0000225120Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272559.BF3101.

Structurei

3D structure databases

ProteinModelPortaliQ5LAS7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0817.
KOiK01159.
OMAiRYDIMEA.
OrthoDBiEOG6RG044.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiPS01321. RUVC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5LAS7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIQPVKEKII LGIDPGTTIM GYGVLRVCGT RPEMIAMGII DLRKFGNHYL
60 70 80 90 100
KLRHIHERVL SIIESYLPDE LAIEAPFFGK NVQSMLKLGR AQGVAMAAAL
110 120 130 140 150
SRDIPITEYA PLKIKMAITG NGQASKEQVA DMLQRMLHFA KEDMPVFMDA
160 170 180
TDGLAAAYCH FLQMGRPVME KGYSGWKDFI AKNPERVK
Length:188
Mass (Da):20,995
Last modified:June 21, 2005 - v1
Checksum:i38C131771C78B639
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR626927 Genomic DNA. Translation: CAH08796.1.
RefSeqiWP_005789290.1. NC_003228.3.
YP_212714.1. NC_003228.3.

Genome annotation databases

EnsemblBacteriaiCAH08796; CAH08796; BF9343_3015.
KEGGibfs:BF9343_3015.
PATRICi21042834. VBIBacFra29119_3136.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR626927 Genomic DNA. Translation: CAH08796.1.
RefSeqiWP_005789290.1. NC_003228.3.
YP_212714.1. NC_003228.3.

3D structure databases

ProteinModelPortaliQ5LAS7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272559.BF3101.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAH08796; CAH08796; BF9343_3015.
KEGGibfs:BF9343_3015.
PATRICi21042834. VBIBacFra29119_3136.

Phylogenomic databases

eggNOGiCOG0817.
KOiK01159.
OMAiRYDIMEA.
OrthoDBiEOG6RG044.

Enzyme and pathway databases

BioCyciBFRA272559:GKF0-3058-MONOMER.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343.

Entry informationi

Entry nameiRUVC_BACFN
AccessioniPrimary (citable) accession number: Q5LAS7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: June 21, 2005
Last modified: May 27, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.