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Q5LAS7 (RUVC_BACFN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RuvC

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene names
Name:ruvC
Ordered Locus Names:BF3101
OrganismBacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]
Taxonomic identifier272559 [NCBI]
Taxonomic lineageBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides

Protein attributes

Sequence length188 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00034

Sequence similarities

Belongs to the RuvC family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 188188Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034
PRO_0000225120

Sites

Metal binding141Magnesium By similarity
Metal binding741Magnesium By similarity
Metal binding1491Magnesium By similarity
Metal binding1521Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5LAS7 [UniParc].

Last modified June 21, 2005. Version 1.
Checksum: 38C131771C78B639

FASTA18820,995
        10         20         30         40         50         60 
MIQPVKEKII LGIDPGTTIM GYGVLRVCGT RPEMIAMGII DLRKFGNHYL KLRHIHERVL 

        70         80         90        100        110        120 
SIIESYLPDE LAIEAPFFGK NVQSMLKLGR AQGVAMAAAL SRDIPITEYA PLKIKMAITG 

       130        140        150        160        170        180 
NGQASKEQVA DMLQRMLHFA KEDMPVFMDA TDGLAAAYCH FLQMGRPVME KGYSGWKDFI 


AKNPERVK 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR626927 Genomic DNA. Translation: CAH08796.1.
RefSeqYP_212714.1. NC_003228.3.

3D structure databases

ProteinModelPortalQ5LAS7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272559.BF3101.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAH08796; CAH08796; BF9343_3015.
GeneID3288290.
KEGGbfs:BF3101.
PATRIC21042834. VBIBacFra29119_3136.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0817.
KOK01159.
OMAILHMIKL.
OrthoDBEOG6RG044.
ProtClustDBPRK00039.

Enzyme and pathway databases

BioCycBFRA272559:GKF0-3058-MONOMER.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
HAMAPMF_00034. RuvC.
InterProIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSPR00696. RSOLVASERUVC.
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00228. ruvC. 1 hit.
PROSITEPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRUVC_BACFN
AccessionPrimary (citable) accession number: Q5LAS7
Entry history
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: June 21, 2005
Last modified: April 16, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families