Reviewed, UniProtKB/Swiss-Prot Q5LAD5 (PURA_BACFN)

Names and origin

Protein names

Recommended name:
    Adenylosuccinate synthetase
    EC=6.3.4.4
Alternative name(s):
    IMP--aspartate ligase
    AdSS
    AMPSase

Gene names

Name:	purA
Ordered Locus Names:	BF3243

Organism

Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]

Taxonomic identifier

272559 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis. HAMAP MF_00011
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP adenylosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	Adenylosuccinate synthetase HAMAP MF_00011		PRO_0000224256
Regions
	Nucleotide binding	12 – 18	7	GTP Potential
Sites
	Active site	140	1	By similarity
	Active site	147	1	By similarity
	Metal binding	13	1	Magnesium By similarity
	Metal binding	40	1	Magnesium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5LAD5-1 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: B9B7D4A8343569F6	FASTA	423	46,799
	10 20 30 40 50 60 MKVDVLLGLQ WGDEGKGKVV DVLTPKYDVV ARFQGGPNAG HTLEFEGQKY VLRSIPSGIF 70 80 90 100 110 120 QGDKVNIIGN GVVLDPALFK AEAEALEASG HNLKERLHIS KKAHLILPTH RILDAAYEAA 130 140 150 160 170 180 KGDAKVGTTG KGIGPTYTDK VSRNGVRVGD ILHNFEQKYA AAKARHEQIL KGLNYEYDLT 190 200 210 220 230 240 ELEKAWFEGI EYLKQFQLVD SEHEINGLLD NGKSILCEGA QGTMLDIDFG SYPFVTSSNT 250 260 270 280 290 300 VCAGACTGLG VAPNKIGDVY GIFKAYCTRV GSGPFPTELF DKTGDQICTL GHEFGSVTGR 310 320 330 340 350 360 KRRCGWVDLV ALKYSIMVNG VTKLIMMKSD VLDTFETIKA CVAYKMNGEE IDYFPYDITD 370 380 390 400 410 420 EVEPIYVELP GWQTDMTKMQ SEDEFPEEFN AYLSFLEEQL GVQIKIVSVG PDREQTIIRY TEE

References

[1]

"Extensive DNA inversions in the B. fragilis genome control variable gene expression." Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V., Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A., Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A. , Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J., Barrell B.G., Parkhill J. Science 307:1463-1465(2005) [PubMed: 15746427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR626927 Genomic DNA. Translation: CAH08938.1.
RefSeq	YP_212856.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5LAD5.
Genome annotation databases
GeneID	3289325.
GenomeReviews	Gene locus BF3243 in contig CR626927_GR.
KEGG	bfs:BF3243.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5LAD5.
OMA	IPVCVAY.
Enzyme and pathway databases
BioCyc	BFRA272559:BF3243-MON.
Family and domain databases
HAMAP	MF_00011. [Tree]
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
ProDom	PD001188. Asucc_synthtase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00184. purA. 1 hit.
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA_BACFN

Accession

Primary (citable) accession number: Q5LAD5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	June 21, 2005
Last modified:	November 3, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)