Reviewed, UniProtKB/Swiss-Prot Q5L9L6 (TYSY_BACFN)

Names and origin

Protein names

Recommended name:
    Thymidylate synthase
      Short name=TS
      Short name=TSase
    EC=2.1.1.45

Gene names

Name:	thyA
Ordered Locus Names:	BF3523

Organism

Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]

Taxonomic identifier

272559 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type thyA subfamily.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Thymidylate synthase HAMAP MF_00008		PRO_1000000577
Sites
	Active site	146	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5L9L6-1 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: 1D8A4F82BB1754BD	FASTA	264	30,365
	10 20 30 40 50 60 MKQYLDLLDR VLTEGIEKGD RTGTGTISVF GHQMRFNLDE GFPCLTTKKL HLKSIIYELL 70 80 90 100 110 120 WFLKGDTNVK YLQDHGVRIW NEWADETGDL GHIYGYQWRS WPTYDGGFID QISEAVDAIK 130 140 150 160 170 180 HNPDSRRIIV SAWNVGDLDH MNLPPCHAFF QFYVANGRLS LQLYQRSADI FLGVPFNIAS 190 200 210 220 230 240 YALLLQMMAQ VTGLQAGDFI HTLGDAHIYL NHLEQVKLQL SREPRPLPQM KINPDVKNIF 250 260 DFQFEDFELV NYDPHPHIAG KVAV

References

[1]

"Extensive DNA inversions in the B. fragilis genome control variable gene expression." Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V., Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A., Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A. , Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J., Barrell B.G., Parkhill J. Science 307:1463-1465(2005) [PubMed: 15746427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR626927 Genomic DNA. Translation: CAH09211.1.
RefSeq	YP_213125.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5L9L6.
Genome annotation databases
GeneID	3287200.
GenomeReviews	Gene locus BF3523 in contig CR626927_GR.
KEGG	bfs:BF3523.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5L9L6.
OMA	LGPVYGH.
Enzyme and pathway databases
BioCyc	BFRA272559:BF3523-MON.
Family and domain databases
HAMAP	MF_00008. [Tree]
InterPro	IPR000398. Thymidylat_synth_C. [Graphical view]
Gene3D	G3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHER	PTHR11549:SF2. Thymidylat_synth_C. 1 hit.
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
ProDom	PD001180. Thymidylat_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_BACFN

Accession

Primary (citable) accession number: Q5L9L6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 21, 2005
Last modified:	November 3, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)