Reviewed, UniProtKB/Swiss-Prot Q5L9I8 (ILVD_BACFN)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase
      Short name=DAD
    EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	BF3551

Organism

Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]

Taxonomic identifier

272559 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	600 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 600	600	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000225372
Sites
	Metal binding	123	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	192	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q5L9I8-1 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: 1884972FD7F415DA	FASTA	600	64,351
	10 20 30 40 50 60 MKKQLRSSFS TQGRRMAGAR ALWAANGMKK EQLGKPIIAI VNSFTQFVPG HVHLHEIGQL 70 80 90 100 110 120 VKKEIEKLGC FAAEFNTIAI DDGIAMGHDG MLYSLPSRDI IADSVEYMVN AHKADAMVCI 130 140 150 160 170 180 SNCDKITPGM LMAAMRLNIP TVFVSGGPME AGELDGQHLD LIDAMIKSAD ESVSDEEVSK 190 200 210 220 230 240 IENRACPTCG CCSGMFTANS MNCLNEAIGL ALPGNGTIVA THANRTQLFK DAAKLIVENT 250 260 270 280 290 300 YKYYRDGDES VLPRSIATRE AFLNAMTLDI AMGGSTNTVL HLLAIAHEAE ANFKMDDIDM 310 320 330 340 350 360 LSRKSPCLCK VAPNTQKYHI QDVNRAGGIM GIMGQLAKAG LIDTSVVRID GMTLGEAIDK 370 380 390 400 410 420 YDITSPNVCE EAIKKYKSAA AGKFNLVLGS QDVYYKELDT DRAEGCIRDI EHAYSKDGGL 430 440 450 460 470 480 AVLKGNIAQD GCVVKTAGVD ESIWKFTGPA KVFDSQEAAC NGILGGKVVS GDVVVITHEG 490 500 510 520 530 540 PKGGPGMQEM LYPTSYIKSR HLGKECALIT DGRFSGGTSG LSIGHISPEA AAGGNIGKIK 550 560 570 580 590 600 DGDIIEINIP ERTINVRLTD EELAARPMTP VTRERYVPKS LKAYASMVSS ADKGAVRLID

References

[1]

"Extensive DNA inversions in the B. fragilis genome control variable gene expression." Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V., Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A., Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A. , Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J., Barrell B.G., Parkhill J. Science 307:1463-1465(2005) [PubMed: 15746427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR626927 Genomic DNA. Translation: CAH09239.1.
RefSeq	YP_213153.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5L9I8.
Genome annotation databases
GeneID	3289185.
GenomeReviews	Gene locus BF3551 in contig CR626927_GR.
KEGG	bfs:BF3551.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5L9I8.
OMA	SRKVPCL.
Enzyme and pathway databases
BioCyc	BFRA272559:BF3551-MON.
Family and domain databases
HAMAP	MF_00012. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
ProDom	PD002691. ILVD_EDD_family. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_BACFN

Accession

Primary (citable) accession number: Q5L9I8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	June 21, 2005
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)