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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathway:iN(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei51 – 511S-adenosyl-L-methionineUniRule annotation
Binding sitei76 – 761S-adenosyl-L-methionineUniRule annotation
Binding sitei103 – 1031S-adenosyl-L-methionineUniRule annotation
Active sitei125 – 1251By similarity
Binding sitei125 – 1251S-adenosyl-L-methionineUniRule annotation
Binding sitei129 – 1291SubstrateUniRule annotation
Binding sitei159 – 1591SubstrateUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBFRA272559:GKF0-3726-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:BF3770
OrganismiBacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343)
Taxonomic identifieri272559 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
ProteomesiUP000006731 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 252252tRNA (guanine-N(7)-)-methyltransferasePRO_0000288123Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi272559.BF3770.

Structurei

3D structure databases

ProteinModelPortaliQ5L8Y2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni199 – 2024Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
KOiK03439.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q5L8Y2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKNKLEKFA DMASYPHVFE YPYSAVDNVP FDMKGKWHKE FFKNDNPIVL
60 70 80 90 100
ELGCGRGEYT VGLGRMFPDK NFIAVDIKGA RMWTGATESF QTGMKNVAFL
110 120 130 140 150
RTNIEIIDRF FAEGEVSEIW LTFSDPQMKK ATKRLTSTYF MERYRKFLVS
160 170 180 190 200
NGIIHLKTDS NFMFTYTKYM IEENGLPVEF ITEDLYHSDL VDDILGIKTY
210 220 230 240 250
YEQQWLDRGL SIKYIKFLLP QEGELREPDI EIELDSYRSY NRSKRSGLQT

SK
Length:252
Mass (Da):29,543
Last modified:May 29, 2007 - v2
Checksum:i3D7423B65A967ABB
GO

Sequence cautioni

The sequence CAH09450.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR626927 Genomic DNA. Translation: CAH09450.1. Different initiation.
RefSeqiWP_041926264.1. NC_003228.3.

Genome annotation databases

EnsemblBacteriaiCAH09450; CAH09450; BF9343_3669.
KEGGibfs:BF9343_3669.
PATRICi21044298. VBIBacFra29119_3854.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR626927 Genomic DNA. Translation: CAH09450.1. Different initiation.
RefSeqiWP_041926264.1. NC_003228.3.

3D structure databases

ProteinModelPortaliQ5L8Y2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272559.BF3770.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAH09450; CAH09450; BF9343_3669.
KEGGibfs:BF9343_3669.
PATRICi21044298. VBIBacFra29119_3854.

Phylogenomic databases

eggNOGiCOG0220.
KOiK03439.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciBFRA272559:GKF0-3726-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343.

Entry informationi

Entry nameiTRMB_BACFN
AccessioniPrimary (citable) accession number: Q5L8Y2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: July 22, 2015
This is version 60 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.