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Q5L8Y2

- TRMB_BACFN

UniProt

Q5L8Y2 - TRMB_BACFN

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Bacteroides fragilis (strain ATCC 25285 / NCTC 9343)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 2 (29 May 2007)
      Previous versions | rss
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    • Comment

    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei51 – 511S-adenosyl-L-methionineUniRule annotation
    Binding sitei76 – 761S-adenosyl-L-methionineUniRule annotation
    Binding sitei103 – 1031S-adenosyl-L-methionineUniRule annotation
    Active sitei125 – 1251By similarity
    Binding sitei125 – 1251S-adenosyl-L-methionineUniRule annotation
    Binding sitei129 – 1291SubstrateUniRule annotation
    Binding sitei159 – 1591SubstrateUniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciBFRA272559:GKF0-3726-MONOMER.
    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    Name:trmBUniRule annotation
    Ordered Locus Names:BF3770
    OrganismiBacteroides fragilis (strain ATCC 25285 / NCTC 9343)
    Taxonomic identifieri272559 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
    ProteomesiUP000006731: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 252252tRNA (guanine-N(7)-)-methyltransferasePRO_0000288123Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi272559.BF3770.

    Structurei

    3D structure databases

    ProteinModelPortaliQ5L8Y2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni199 – 2024Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0220.
    KOiK03439.
    OrthoDBiEOG6K6VBC.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q5L8Y2-1 [UniParc]FASTAAdd to Basket

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    MGKNKLEKFA DMASYPHVFE YPYSAVDNVP FDMKGKWHKE FFKNDNPIVL    50
    ELGCGRGEYT VGLGRMFPDK NFIAVDIKGA RMWTGATESF QTGMKNVAFL 100
    RTNIEIIDRF FAEGEVSEIW LTFSDPQMKK ATKRLTSTYF MERYRKFLVS 150
    NGIIHLKTDS NFMFTYTKYM IEENGLPVEF ITEDLYHSDL VDDILGIKTY 200
    YEQQWLDRGL SIKYIKFLLP QEGELREPDI EIELDSYRSY NRSKRSGLQT 250
    SK 252
    Length:252
    Mass (Da):29,543
    Last modified:May 29, 2007 - v2
    Checksum:i3D7423B65A967ABB
    GO

    Sequence cautioni

    The sequence CAH09450.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR626927 Genomic DNA. Translation: CAH09450.1. Different initiation.
    RefSeqiYP_213359.1. NC_003228.3.

    Genome annotation databases

    EnsemblBacteriaiCAH09450; CAH09450; BF9343_3669.
    GeneIDi3287859.
    KEGGibfs:BF3770.
    PATRICi21044298. VBIBacFra29119_3854.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR626927 Genomic DNA. Translation: CAH09450.1 . Different initiation.
    RefSeqi YP_213359.1. NC_003228.3.

    3D structure databases

    ProteinModelPortali Q5L8Y2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272559.BF3770.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAH09450 ; CAH09450 ; BF9343_3669 .
    GeneIDi 3287859.
    KEGGi bfs:BF3770.
    PATRICi 21044298. VBIBacFra29119_3854.

    Phylogenomic databases

    eggNOGi COG0220.
    KOi K03439.
    OrthoDBi EOG6K6VBC.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .
    BioCyci BFRA272559:GKF0-3726-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 25285 / NCTC 9343.

    Entry informationi

    Entry nameiTRMB_BACFN
    AccessioniPrimary (citable) accession number: Q5L8Y2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 29, 2007
    Last sequence update: May 29, 2007
    Last modified: October 1, 2014
    This is version 56 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3