Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q5L8Y2 (TRMB_BACFN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:BF3770
OrganismBacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]
Taxonomic identifier272559 [NCBI]
Taxonomic lineageBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides

Protein attributes

Sequence length252 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Sequence caution

The sequence CAH09450.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 252252tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000288123

Regions

Region199 – 2024Substrate binding By similarity

Sites

Active site1251 By similarity
Binding site511S-adenosyl-L-methionine By similarity
Binding site761S-adenosyl-L-methionine By similarity
Binding site1031S-adenosyl-L-methionine By similarity
Binding site1251S-adenosyl-L-methionine By similarity
Binding site1291Substrate By similarity
Binding site1591Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q5L8Y2 [UniParc].

Last modified May 29, 2007. Version 2.
Checksum: 3D7423B65A967ABB

FASTA25229,543
        10         20         30         40         50         60 
MGKNKLEKFA DMASYPHVFE YPYSAVDNVP FDMKGKWHKE FFKNDNPIVL ELGCGRGEYT 

        70         80         90        100        110        120 
VGLGRMFPDK NFIAVDIKGA RMWTGATESF QTGMKNVAFL RTNIEIIDRF FAEGEVSEIW 

       130        140        150        160        170        180 
LTFSDPQMKK ATKRLTSTYF MERYRKFLVS NGIIHLKTDS NFMFTYTKYM IEENGLPVEF 

       190        200        210        220        230        240 
ITEDLYHSDL VDDILGIKTY YEQQWLDRGL SIKYIKFLLP QEGELREPDI EIELDSYRSY 

       250 
NRSKRSGLQT SK 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR626927 Genomic DNA. Translation: CAH09450.1. Different initiation.
RefSeqYP_213359.1. NC_003228.3.

3D structure databases

ProteinModelPortalQ5L8Y2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING272559.BF3770.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAH09450; CAH09450; BF9343_3669.
GeneID3287859.
KEGGbfs:BF3770.
PATRIC21044298. VBIBacFra29119_3854.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
KOK03439.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycBFRA272559:GKF0-3726-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_BACFN
AccessionPrimary (citable) accession number: Q5L8Y2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: April 16, 2014
This is version 53 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways