Reviewed, UniProtKB/Swiss-Prot Q5L8E4 (HUTI_BACFN)

Names and origin

Protein names

Recommended name:
    Imidazolonepropionase
    EC=3.5.2.7
Alternative name(s):
    Imidazolone-5-propionate hydrolase

Gene names

Name:	hutI
Ordered Locus Names:	BF3968

Organism

Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]

Taxonomic identifier

272559 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+. HAMAP MF_00372
Cofactor	Binds 1 zinc or iron ion per subunit By similarity.
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3. HAMAP MF_00372
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the hutI family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazolonepropionase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Imidazolonepropionase HAMAP MF_00372		PRO_0000306436
Sites
	Metal binding	80	1	Zinc or iron By similarity
	Metal binding	82	1	Zinc or iron By similarity
	Metal binding	252	1	Zinc or iron By similarity
	Metal binding	326	1	Zinc or iron By similarity
	Binding site	89	1	Substrate By similarity
	Binding site	102	1	Substrate By similarity
	Binding site	152	1	Substrate By similarity
	Binding site	187	1	Substrate By similarity
	Binding site	255	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5L8E4-1 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: B80F0466F51DEBE6	FASTA	417	45,450
	10 20 30 40 50 60 MSENLIIFNA KVVTPLGFSA RKGKEMAELR ILEKATVEVV DGIITYVGPN RGEVRDGYYH 70 80 90 100 110 120 HFWHYNARGK CLLPGFVDSH THFVFGGERA EEFSWRLKGE SYMSIMQRGG GIASTVQATR 130 140 150 160 170 180 ELSFIHLRSK AEGLLKKMTA MGITTVEGKS GYGLNRETEL LQLKVMRSLN KDEGVRVDIV 190 200 210 220 230 240 PTFLGAHALP DEYKERPDDY IDFLIRELLP VIQRDSLAEF CDVFCEEGVF SIEQSRRLLT 250 260 270 280 290 300 AAGDYGLLPK LHADEIVPLG GAELAAELGA VSADHLLHAS DAGIEAMARK GVVATLLPLT 310 320 330 340 350 360 AFALKEPYAR GRDMIDAGCA VALATDLNPG SCFSGSIPLT FALACIHMQL TVEEAITALT 370 380 390 400 410 LNGAAALNRA DSIGSIEVGK KGDFVVLDSD NYHILPYYVG MNCVNTTIKG GMLYPSV

References

[1]

"Extensive DNA inversions in the B. fragilis genome control variable gene expression." Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V., Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A., Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A. , Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J., Barrell B.G., Parkhill J. Science 307:1463-1465(2005) [PubMed: 15746427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR626927 Genomic DNA. Translation: CAH09644.1.
RefSeq	YP_213547.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q5L8E4.
Genome annotation databases
GeneID	3289168.
GenomeReviews	Gene locus BF3968 in contig CR626927_GR.
KEGG	bfs:BF3968.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q5L8E4.
OMA	MNMACTL.
Enzyme and pathway databases
BioCyc	BFRA272559:BF3968-MON.
Family and domain databases
HAMAP	MF_00372. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR005920. HutI. [Graphical view]
Pfam	PF01979. Amidohydro_1. 2 hits. [Graphical view]
TIGRFAMs	TIGR01224. hutI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUTI_BACFN

Accession

Primary (citable) accession number: Q5L8E4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 2, 2007
Last sequence update:	June 21, 2005
Last modified:	November 3, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)