Q5L8A7 (EFG_BACFN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor G
Short name=EF-G

Gene names

Name:	fusA
Ordered Locus Names:	BF4005

Organism

Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) [Complete proteome] [HAMAP]

Taxonomic identifier

272559 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	705 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome By similarity. HAMAP MF_00054_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00054_B.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 705	705	Elongation factor G HAMAP MF_00054_B		PRO_0000225193
Regions
	Nucleotide binding	16 – 23	8	GTP By similarity
	Nucleotide binding	84 – 88	5	GTP By similarity
	Nucleotide binding	138 – 141	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q5L8A7 [UniParc]. Last modified June 21, 2005. Version 1. Checksum: F61416FD170E1961	FASTA	705	77,508
	10 20 30 40 50 60 MAKNDLHLTR NIGIMAHIDA GKTTTSERIL FYTGLTHKIG EVHDGAATMD WMEQEQERGI 70 80 90 100 110 120 TITSAATTTR WKYAGDTYKI NLIDTPGHVD FTAEVERSLR ILDGAVAAYC AVGGVEPQSE 130 140 150 160 170 180 TVWRQADKYN VPRIAYVNKM DRSGADFFEV VRQMKAVLGA NPCPVVIPIG AEENFKGLVD 190 200 210 220 230 240 LIKMKAIYWH DETMGADYTI EEIPANLVDE ANEWRDKMLE KVAEFDDALM EKYFDDPSTI 250 260 270 280 290 300 TEEEVLRALR NATVQMAVVP MLCGSSFKNK GVQTLLDYVC AFLPSPLDAE NVVGTNPDTG 310 320 330 340 350 360 AEEDRKPSED DKTSALAFKI ATDPYVGRLT FFRVYSGKIE AGSYIYNSRS GKKERVSRLF 370 380 390 400 410 420 QMHSNKQNPV EVIGAGDIGA GVGFKDIHTG DTLCDETAPI VLESMDFPEP VIGIAVEPKT 430 440 450 460 470 480 QKDMDKLSNG LAKLAEEDPT FTVKTDEQTG QTVISGMGEL HLDIIIDRLK REFKVECNQG 490 500 510 520 530 540 KPQVNYKEAI TKTVNLREVY KKQSGGRGKF ADIIVNIGPV DEDFTQGGLQ FVDEVKGGNI 550 560 570 580 590 600 PKEFIPSVQK GFQTAMKNGV LAGYPLDSLK VTLVDGSFHP VDSDQLSFEI CAIQAYKNAC 610 620 630 640 650 660 AKAGPVLMEP IMKLEVVTPE ENMGDVIGDL NKRRGQVEGM ESSRSGARIV KAMVPLAEMF 670 680 690 700 GYVTALRTIT SGRATSSMVY SHHAQVSSSI AKAVLEEVKG RADLL

References

[1]

"Extensive DNA inversions in the B. fragilis genome control variable gene expression." Cerdeno-Tarraga A.-M., Patrick S., Crossman L.C., Blakely G., Abratt V., Lennard N., Poxton I., Duerden B., Harris B., Quail M.A., Barron A., Clark L., Corton C., Doggett J., Holden M.T.G., Larke N., Line A., Lord A. , Norbertczak H., Ormond D., Price C., Rabbinowitsch E., Woodward J., Barrell B.G., Parkhill J. Science 307:1463-1465(2005) [PubMed: 15746427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25285 / NCTC 9343.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR626927 Genomic DNA. Translation: CAH09681.1.
RefSeq	YP_213584.1. NC_003228.3.
3D structure databases
ProteinModelPortal	Q5L8A7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q5L8A7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3286685.
GenomeReviews	Gene locus BF4005 in contig CR626927_GR.
KEGG	bfs:BF4005.
PATRIC	21044814. VBIBacFra29119_4098.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0480.
HOGENOM	HBG737692.
OMA	CNEWREK.
ProtClustDB	PRK12739.
Enzyme and pathway databases
BioCyc	BFRA272559:BF4005-MONOMER.
Family and domain databases
HAMAP	MF_00054_B. EF-G_EF-2_B. [Tree]
InterPro	IPR009022. Elongation_fac_G/III/V. IPR000795. ProtSyn_GTP-bd. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR005225. Small_GTP-bd_dom. IPR004540. Transl_elong_EFG/EF2. IPR000640. Transl_elong_EFG/EF2_C. IPR005517. Transl_elong_EFG/EF2_IV. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.30.70.240. Transl_elong_EFG/EF2_C. 1 hit.
KO	K02355.
Pfam	PF00679. EFG_C. 1 hit. PF03764. EFG_IV. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SMART	SM00838. EFG_C. 1 hit. SM00889. EFG_IV. 1 hit. [Graphical view]
SUPFAM	SSF54980. EFG_III_V. 2 hits. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00484. EF-G. 1 hit. TIGR00231. Small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFG_BACFN

Accession

Primary (citable) accession number: Q5L8A7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	June 21, 2005
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program